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26
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1 library(ggplot2)
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2
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3 args <- commandArgs(trailingOnly = TRUE)
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4
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5 input = args[1]
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6 outfile = args[2]
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104
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7 gene = args[3]
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26
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8
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56
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9 print("---------------- read input ----------------")
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10
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49
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11 dat = read.table(input, sep="\t", fill=T, header=T, quote="")
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12
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56
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13 print("---------------- as numeric ----------------")
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49
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14
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15 mutations.at.position = as.numeric(dat[1,])
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16 aa.at.position = as.numeric(dat[2,])
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26
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17
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56
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18 print("---------------- freq data.frame ----------------")
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19
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49
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20 dat_freq = mutations.at.position / aa.at.position
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26
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21 dat_dt = data.frame(i=1:length(dat_freq), freq=dat_freq)
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22
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56
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23 print("---------------- plot ----------------")
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24
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26
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25 m = ggplot(dat_dt, aes(x=i, y=freq)) + theme(axis.text.x = element_text(angle = 90, hjust = 1))
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82
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26 m = m + geom_bar(stat="identity", colour = "black", fill = "darkgrey", alpha=0.8) + scale_x_continuous(breaks=1:length(dat_freq), labels=1:length(dat_freq))
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49
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27 m = m + annotate("segment", x = 0.5, y = -0.05, xend=26.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 13, y = -0.1, label="FR1")
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28 m = m + annotate("segment", x = 26.5, y = -0.07, xend=38.5, yend=-0.07, colour="darkblue", size=1) + annotate("text", x = 32.5, y = -0.15, label="CDR1")
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29 m = m + annotate("segment", x = 38.5, y = -0.05, xend=55.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 47, y = -0.1, label="FR2")
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30 m = m + annotate("segment", x = 55.5, y = -0.07, xend=65.5, yend=-0.07, colour="darkblue", size=1) + annotate("text", x = 60.5, y = -0.15, label="CDR2")
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31 m = m + annotate("segment", x = 65.5, y = -0.05, xend=104.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 85, y = -0.1, label="FR3")
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104
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32 m = m + expand_limits(y=c(-0.1,1)) + xlab("AA position") + ylab("Frequency") + ggtitle(paste(gene, "AA mutation frequency"))
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57
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33
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34 print("---------------- write/print ----------------")
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35
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104
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36 write.table(dat_dt, paste(dirname(outfile), "/aa_histogram_", gene, ".txt", sep=""), sep="\t",quote=F,row.names=F,col.names=T)
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26
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37 png(filename=outfile, width=1280, height=720)
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38 print(m)
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39 dev.off()
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