Mercurial > repos > davidvanzessen > igblast_human
diff igblastn.xml @ 63:7997fea1f16a draft default tip
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author | davidvanzessen |
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date | Thu, 06 Nov 2014 03:31:15 -0500 |
parents | a700a552f031 |
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--- a/igblastn.xml Wed Aug 20 10:04:09 2014 -0400 +++ b/igblastn.xml Thu Nov 06 03:31:15 2014 -0500 @@ -1,16 +1,82 @@ <tool id="igblastn" name="igBLASTn" version="0.1.0"> <description> </description> - <command interpreter="bash"> - igblastn.sh $input $output + <command interpreter="python"> + igblast_post.py --input $input --output $output </command> <inputs> <param name="input" type="data" format="fasta" label="Fasta file"/> </inputs> <outputs> - <data name="output" format="text" label="${input.name}-igBLASTn Report"/> + <data name="output" format="tabular" type="data" label="${input.name}-igBLASTn Report"/> <!--<data name="log" format="text" label="log"/>--> </outputs> + <requirements> + <requirement type="package" version="1.0.0">igBlastn</requirement> + </requirements> <help> - Step 1 of the Immune Repertoire tools, generates a report to be parsed with the "igparse" tool. +============ +iReport +============ + +This tool uses the online igBLAST website hosted by NCBI to blast a FASTA file, it retrieves the result and generates a convenient tabular format for further processing. + +**NOTE** + +.. class:: warningmark + +- Everything goes through the servers of NCBI, so if you have sensitive data that that isn't allowed to leave your local network, this isn't the tool the use. + +**USAGE** + +.. class:: infomark + +- This tool uses a free service provided by NCBI, and although there doesn't seem to be any restrictions on usage, avoid unnecessary usage to lighten the load on NCBI's servers. + + +**INPUT** + +This tool accepts FASTA files as input: + +:: + + >lcl|FLN1FA002RWEZA.1| + ggctggagtgggtttcatacattagtagtaatagtggtgccatatactacgcagactctgtgaagggccgattcaccatc + tccagaaacaatgccaaggactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgc + gagagcgatcccccggtattactatgatactagtggcccaaacgactactggggccagggaaccctggtcaccgtctcct + cag + >lcl|FLN1FA001BLION.1| + aggcttgagtggatgggatggatcaacgctggcaatggtaacacaaaatattcacagaagttccagggcagagtcaccat + taccagggacacatccgcgagcacagcctacatggagctgagcagcctgagatctgaagacacggctgtgtattactgtg + cgagagtgggcagcagctggtctgatgcttttgattatctggggccaagggacaatggtcaccgtctcctcag + +**OUTPUT** + +The following data is used for ARGalaxy + ++-----------------+----------------------------------------------+ +| Column name | Column contents | ++-----------------+----------------------------------------------+ +| ID | The Sequence ID provided by the sequencer. | ++-----------------+----------------------------------------------+ +| VDJ Frame | In-frame/Out-frame | ++-----------------+----------------------------------------------+ +| Top V Gene | The best matching V gene found. | ++-----------------+----------------------------------------------+ +| Top D Gene | The best matching D gene found. | ++-----------------+----------------------------------------------+ +| Top J Gene | The best matching J gene found. | ++-----------------+----------------------------------------------+ +| CDR3 Seq | The CDR3 region. | ++-----------------+----------------------------------------------+ +| CDR3 Length | The length of the CDR3 region. | ++-----------------+----------------------------------------------+ +| CDR3 Seq DNA | The CDR3 sequence region. | ++-----------------+----------------------------------------------+ +| CDR3 Length DNA | The length of the CDR3 sequence region. | ++-----------------+----------------------------------------------+ +| Functionality | If sequence is productive/unproductive | ++-----------------+----------------------------------------------+ + + </help> </tool>