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0
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1 #!/bin/bash
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2
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5
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3 #export IGDATA=/home/david/Galaxy/galaxy-dist/toolsheddependencies/igBlastn/1.0.0/davidvanzessen/igblast_human/1c64c977624e/ncbi-igblast-1.0.0/;
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2
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4
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5 clonalType=${@:(-3):1}
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0
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6 html=${@:(-2):1}
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7 imageDir=${@:(-1):1}
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8 dir="$(cd "$(dirname "$0")" && pwd)"
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2
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9 fileCount=`expr $# - 3`
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0
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10 array=("$@")
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11 echo "<html><h3>Progress</h3><table><tr><td>info</td></tr>" > $html
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12 echo "<tr><td>-----------------------------------</td></tr>" >> $html
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13 limit=`expr $fileCount / 2`
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14
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15 function blastAndParse {
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16 echo "<tr><td>Starting blast of $2</td></tr>" >> $html
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17 fileName=$(basename $1)
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18 $IGDATA/bin/igblastn -germline_db_V $IGDATA/database/human_gl_V -germline_db_J $IGDATA/database/human_gl_J -germline_db_D $IGDATA/database/human_gl_D -domain_system imgt -query $1 -auxiliary_data $IGDATA/optional_file/human_gl.aux -show_translation -outfmt 3 > $PWD/$fileName
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19 echo "<tr><td>Finished blast of $2</td></tr>" >> $html
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20
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21 echo "<tr><td>Starting parse of $2</td></tr>" >> $html
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22 parsedFileName="${fileName%.*}"
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23 parsedFileName="$parsedFileName.parsed"
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24 perl $dir/igparse.pl $PWD/$fileName 0 | grep -v "D:" | cut -f2- > $parsedFileName
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25 echo "<tr><td>Finished parse of $2</td></tr>" >> $html
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26 }
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27
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28 for ((i=0;i<$fileCount;i=$((i+2))))
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29 do
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30 next=$((i+1))
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31 blastAndParse ${array[$i]} ${array[$next]} &
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32 done
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33 wait
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34
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35
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36
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37 echo "<tr><td>-----------------------------------</td></tr>" >> $html
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38 echo "<tr><td>merging</td></tr>" >> $html
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39
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40 count=0
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41 for ((i=0;i<$fileCount;i=$((i+2))))
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42 do
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43 id=$((i+1))
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44 place=$((count+limit))
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45 fn=$(basename ${array[$i]})
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46 fn="${fn%.*}"
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47 mergeInputs[$count]="$PWD/$fn.parsed"
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48 mergeIDs[$place]=${array[$id]}
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49 count=$((count+1))
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50 done
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51
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52 python $dir/igblastmerge.py --input ${mergeInputs[*]} --id ${mergeIDs[*]} --output $PWD/merged.txt
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53
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54 echo "<tr><td>done</td></tr>" >> $html
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55 echo "<tr><td>-----------------------------------</td></tr>" >> $html
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56 echo "<tr><td>plotting</td></tr>" >> $html
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57
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58
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59 inputFile=$PWD/merged.txt
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60 outputFile=$html
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61 outputDir=$imageDir
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62 mkdir $outputDir
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2
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63 Rscript --verbose $dir/RScript.r $inputFile $outputDir $outputDir $clonalType 2>&1
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64 echo "<html>" > $outputFile
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65 echo "<img src='VPlot.png'/>" >> $outputFile
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66 echo "<img src='DPlot.png'/>" >> $outputFile
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67 echo "<img src='JPlot.png'/>" >> $outputFile
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68
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69 samples=`cat $outputDir/samples.txt`
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70 count=1
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71 echo "<table border='1'>" >> $outputFile
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72 for sample in $samples; do
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73 echo "<tr><td colspan='3' height='100'></td>" >> $outputFile
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74 echo "<tr><td colspan='3'><h1>$sample</h1></td></tr>" >> $outputFile
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75 echo "<tr><td><h2>V-D Heatmap:</h2></td><td><h2>V-J Heatmap:</h2></td><td><h2>D-J Heatmap:</h2></td></tr><tr>" >> $outputFile
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76 mv "$outputDir/HeatmapVD_$sample.png" "$outputDir/VD_$sample.png"
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77 echo "<td><img src='VD_$sample.png'/></td>" >> $outputFile
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78 mv "$outputDir/HeatmapVJ_$sample.png" "$outputDir/VJ_$sample.png"
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79 echo "<td><img src='VJ_$sample.png'/></td>" >> $outputFile
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80 mv "$outputDir/HeatmapDJ_$sample.png" "$outputDir/DJ_$sample.png"
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81 echo "<td><img src='DJ_$sample.png'/></td></tr>" >> $outputFile
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82 count=$((count+1))
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83 done
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84 echo "</table>" >> $outputFile
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2
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85 echo "<h1>$clonalType</h1>" >> $outputFile
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0
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86
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87 echo "</html>" >> $2
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