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author blankenberg
date Thu, 17 Sep 2015 11:43:57 -0400
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1 This repository contains the **Naive Variant Caller** tool.
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5 **What it does**
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7 This tool is a naive variant caller that processes aligned sequencing reads from the BAM format and produces a VCF file containing per position variant calls. This tool allows multiple BAM files to be provided as input and utilizes read group information to make calls for individual samples.
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9 User configurable options allow filtering reads that do not pass mapping or base quality thresholds and minimum per base read depth; user's can also specify the ploidy and whether to consider each strand separately.
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11 In addition to calling alternate alleles based upon simple ratios of nucleotides at a position, per base nucleotide counts are also provided. A custom tag, NC, is used within the Genotype fields. The NC field is a comma-separated listing of nucleotide counts in the form of <nucleotide>=<count>, where a plus or minus character is prepended to indicate strand, if the strandedness option was specified.
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14 ------
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16 **Inputs**
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18 Accepts one or more BAM input files and a reference genome from the built-in list or from a FASTA file in your history.
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21 **Outputs**
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23 The output is in VCF format.
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25 Example VCF output line, without reporting by strand:
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26 ``chrM 16029 . T G,A,C . . AC=15,9,5;AF=0.00155311658729,0.000931869952371,0.000517705529095 GT:AC:AF:NC 0/0:15,9,5:0.00155311658729,0.000931869952371,0.000517705529095:A=9,C=5,T=9629,G=15,``
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28 Example VCF output line, when reporting by strand:
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29 ``chrM 16029 . T G,A,C . . AC=15,9,5;AF=0.00155311658729,0.000931869952371,0.000517705529095 GT:AC:AF:NC 0/0:15,9,5:0.00155311658729,0.000931869952371,0.000517705529095:+T=3972,-A=9,-C=5,-T=5657,-G=15,``
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31 **Options**
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33 Reference Genome:
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35 Ensure that you have selected the correct reference genome, either from the list of built-in genomes or by selecting the corresponding FASTA file from your history.
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37 Restrict to regions:
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39 You can specify any number of regions on which you would like to receive results. You can specify just a chromosome name, or a chromosome name and start postion, or a chromosome name and start and end position for the set of desired regions.
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41 Minimum number of reads needed to consider a REF/ALT:
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43 This value declares the minimum number of reads containing a particular base at each position in order to list and use said allele in genotyping calls. Default is 0.
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45 Minimum base quality:
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47 The minimum base quality score needed for the position in a read to be used for nucleotide counts and genotyping. Default is no filter.
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49 Minimum mapping quality:
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51 The minimum mapping quality score needed to consider a read for nucleotide counts and genotyping. Default is no filter.
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53 Ploidy:
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55 The number of genotype calls to make at each reported position.
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57 Only write out positions with possible alternate alleles:
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59 When set, only positions which have at least one non-reference nucleotide which passes declare filters will be present in the output.
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61 Report counts by strand:
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63 When set, nucleotide counts (NC) will be reported in reference to the aligned read's source strand. Reported as: <strand><BASE>=<COUNT>.
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65 Choose the dtype to use for storing coverage information:
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67 This controls the maximum depth value for each nucleotide/position/strand (when specified). Smaller values require the least amount of memory, but have smaller maximal limits.
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69 +--------+----------------------------+
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70 | name | maximum coverage value |
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71 +========+============================+
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72 | uint8 | 255 |
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73 +--------+----------------------------+
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74 | uint16 | 65,535 |
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75 +--------+----------------------------+
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76 | uint32 | 4,294,967,295 |
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77 +--------+----------------------------+
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78 | uint64 | 18,446,744,073,709,551,615 |
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79 +--------+----------------------------+
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82 ------
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84 **Citation**
0095f76a5d05 planemo upload for repository https://github.com/blankenberg/tools-blankenberg/tree/master/tools/naive_variant_caller commit cf5c0f6fb2651adf72e317a1cf77805fca30d84e
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85
0095f76a5d05 planemo upload for repository https://github.com/blankenberg/tools-blankenberg/tree/master/tools/naive_variant_caller commit cf5c0f6fb2651adf72e317a1cf77805fca30d84e
blankenberg
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86 If you use this tool, please cite Blankenberg D, et al. *In preparation.*