Mercurial > repos > bgruening > sambamba_markdup
comparison Sambamba_markdup.xml @ 0:72025d7986cc draft default tip
planemo upload for repository https://github.com/biod/sambamba commit 99bb4ed496a9cce79ab0a7e613230cf63a44d9f9
| author | bgruening |
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| date | Mon, 10 Feb 2025 19:12:03 +0000 |
| parents | |
| children |
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| -1:000000000000 | 0:72025d7986cc |
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| 1 <tool id="sambamba_markdup" name="Sambamba markdup" version="@TOOL_VERSION@+galaxy@SUFFIX_VERSION@" profile="23.2" license="MIT"> | |
| 2 <description>Finds and marks duplicate reads in BAM files</description> | |
| 3 <macros> | |
| 4 <import>macros.xml</import> | |
| 5 </macros> | |
| 6 <expand macro="requirements"/> | |
| 7 <command detect_errors="exit_code"><![CDATA[ | |
| 8 sambamba markdup | |
| 9 $remove_duplicates | |
| 10 -l $compression_level | |
| 11 '${input_bam}' | |
| 12 '${output}' | |
| 13 --nthreads \${GALAXY_SLOTS:-4} | |
| 14 --tmpdir '\${TMPDIR:-.}' | |
| 15 #if $log_out | |
| 16 2>&1 | tee '${log}' | |
| 17 #end if | |
| 18 ]]></command> | |
| 19 <inputs> | |
| 20 <!-- For deduplication the input bam needs to be coordinate sorted, hence the input file is expected to be coordinate sorted --> | |
| 21 <param name="input_bam" type="data" format="bam" label="Input BAM file"/> | |
| 22 <param argument="--compression-level" type="integer" value="5" min="0" max="9" label="Level of compression for merged BAM file, number from 0 to 9"/> | |
| 23 <param argument="--remove-duplicates" type="boolean" truevalue="-r" falsevalue="" label="Remove duplicates instead of just marking them"/> | |
| 24 <param name="log_out" type="boolean" label="Output log file?" truevalue="yes" falsevalue="no"/> | |
| 25 </inputs> | |
| 26 <outputs> | |
| 27 <data name="output" format="bam" label="${tool.name} on ${on_string}: Output BAM"/> | |
| 28 <data name="log" format="txt" label="${tool.name} on ${on_string}: Output Log"> | |
| 29 <filter>log_out</filter> | |
| 30 </data> | |
| 31 </outputs> | |
| 32 <tests> | |
| 33 <!-- sambamba markdup without dropped reads --> | |
| 34 <test expect_num_outputs="2"> | |
| 35 <param name="input_bam" value="2.bam" ftype="bam"/> | |
| 36 <param name="compression_level" value="5"/> | |
| 37 <param name="remove_duplicates" value="false"/> | |
| 38 <param name="log_out" value="yes"/> | |
| 39 <output name="output" file="2.markdup.bam" ftype="bam" lines_diff="4"/> | |
| 40 <output name="log" file="2.markdup.txt" ftype="txt"/> | |
| 41 </test> | |
| 42 <!-- sambamba markdup with dropped reads --> | |
| 43 <test expect_num_outputs="1"> | |
| 44 <param name="input_bam" value="2.bam" ftype="bam"/> | |
| 45 <param name="compression_level" value="5"/> | |
| 46 <param name="remove_duplicates" value="true"/> | |
| 47 <param name="log_out" value="no"/> | |
| 48 <output name="output" file="2.markdup_removed.bam" ftype="bam" lines_diff="4"/> | |
| 49 </test> | |
| 50 </tests> | |
| 51 <help> | |
| 52 <![CDATA[ | |
| 53 | |
| 54 Sambamba_markdup marks (by default) or removes duplicate reads. To determine whether a read is a duplicate or not, the same criteria as in Picard are used. | |
| 55 | |
| 56 ]]> | |
| 57 </help> | |
| 58 <expand macro="citations"/> | |
| 59 </tool> |
