Mercurial > repos > bgruening > deeptools_plot_coverage
comparison plotCoverage.xml @ 9:4679da05d86c draft
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 13910e1a5ebcfc740c1bc5e38fc676592ef44f11
| author | bgruening |
|---|---|
| date | Mon, 15 Feb 2016 10:06:17 -0500 |
| parents | 1a6309cecf71 |
| children | 752d2d85e6af |
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| 8:02331b8ed2e8 | 9:4679da05d86c |
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| 1 <tool id="deeptools_plot_coverage" name="plotCoverage" version="@WRAPPER_VERSION@.0"> | 1 <tool id="deeptools_plot_coverage" name="plotCoverage" version="@WRAPPER_VERSION@.0"> |
| 2 <description>plots a histogram of coverage on genomic regions </description> | 2 <description>assesses the sequencing depth of BAM files </description> |
| 3 <macros> | 3 <macros> |
| 4 <token name="@BINARY@">plotCoverage</token> | 4 <token name="@BINARY@">plotCoverage</token> |
| 5 <import>deepTools_macros.xml</import> | 5 <import>deepTools_macros.xml</import> |
| 6 </macros> | 6 </macros> |
| 7 <expand macro="requirements"/> | 7 <expand macro="requirements"/> |
| 80 <!--param name="outFileFormat" value="png" /--> | 80 <!--param name="outFileFormat" value="png" /--> |
| 81 <param name="showAdvancedOpt" value="yes" /> | 81 <param name="showAdvancedOpt" value="yes" /> |
| 82 <param name="plotTitle" value="Test Title from Galaxy" /> | 82 <param name="plotTitle" value="Test Title from Galaxy" /> |
| 83 <param name="outRawCounts" value="True" /> | 83 <param name="outRawCounts" value="True" /> |
| 84 <output name="outFileRawCounts" file="plotCoverage_result1.tabular" ftype="tabular" /> | 84 <output name="outFileRawCounts" file="plotCoverage_result1.tabular" ftype="tabular" /> |
| 85 <output name="outFileName" file="plotCoverage_result1.png" ftype="png" compare="sim_size" delta="100" /> | 85 <output name="outFileName" file="plotCoverage_result1.png" ftype="png" compare="sim_size" delta="500" /> |
| 86 </test> | 86 </test> |
| 87 </tests> | 87 </tests> |
| 88 <help> | 88 <help> |
| 89 <![CDATA[ | 89 <![CDATA[ |
| 90 **What it does** | 90 What it does |
| 91 ------------- | |
| 91 | 92 |
| 92 This tools samples 1 million positions in the genome to build | 93 This tool is useful to **assess the sequencing depth** of a given sample. |
| 93 a coverage histogram. Multiple bam files are accepted but all should | 94 It samples 1 million bp, counts the number of overlapping reads and reports |
| 94 correspond to the same genome assembly. | 95 a coverage histogram that tells you how many bases are covered how many times. |
| 96 | |
| 97 **Note:** Multiple BAM files are accepted but all should correspond to the same genome assembly. | |
| 98 | |
| 99 Output | |
| 100 --------- | |
| 101 | |
| 102 The default output is a **panel of two plots** (see below for an example): One is a density plot visualizing the frequencies of read coverages, the other one lets you estimate what fraction of the genome has a depth of sequencing of, for example, 2 overlapping reads or more. | |
| 103 | |
| 104 The optional output is a table where each row represents the number of reads overlapping with a sampled bp. | |
| 105 | |
| 106 .. image:: $PATH_TO_IMAGES/plotCoverage_output.png | |
| 107 :width: 600 | |
| 108 :height: 345 | |
| 109 | |
| 110 Example plot | |
| 111 ----------------- | |
| 112 | |
| 113 .. image:: $PATH_TO_IMAGES/plotCoverage_annotated.png | |
| 114 :width: 600 | |
| 115 :height: 291 | |
| 95 | 116 |
| 96 | 117 |
| 97 @REFERENCES@ | 118 @REFERENCES@ |
| 98 ]]> | 119 ]]> |
| 99 </help> | 120 </help> |
