# HG changeset patch # User bebatut # Date 1454410237 18000 # Node ID c1bd0c5600183a3cbd337a92fc1bc02dd3c54435 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime commit bcbe76277f3e60303faf826f8ce7f018bc663a9a-dirty diff -r 000000000000 -r c1bd0c560018 README.rst --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,7 @@ +QIIME 1.9.1 Galaxy Wrapper +-------------------------- + +Note: Many of these tools output html files that will not display properly +unless sanitization is turned off in Galaxy. This can be done globally via the +`santize_all_html` option in `galaxy.ini` or on a per tool basis using the +`santize_whitelist_file` in `galaxy.ini`. diff -r 000000000000 -r c1bd0c560018 align_seqs.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/align_seqs.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,128 @@ + + using a variety of alignment methods + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + alignment['alignment_method'] == 'pynast' + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + 10.1093/bioinformatics/btp636 + 10.1093/nar/gkh340 + 10.1093/bioinformatics/btp157 + + diff -r 000000000000 -r c1bd0c560018 alpha_diversity.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/alpha_diversity.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,112 @@ + + on each sample in an otu table + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 alpha_rarefaction.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/alpha_rarefaction.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,117 @@ + + + + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 assign_taxonomy.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/assign_taxonomy.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,304 @@ + + + Assign taxonomy to each sequence + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + is the name of the new directory after unzipping the reference OTUs tgz + file. + +-r /rep_set/97_otus.fasta +-t /taxonomy/97_otu_taxonomy.txt + +The consensus taxonomy assignment implemented here is the most detailed lineage +description shared by 90% or more of the sequences within the OTU (this level of +agreement can be adjusted by the user). The full lineage information for each +sequence is one of the output files of the analysis. In addition, a conflict file +records cases in which a phylum-level taxonomy assignment disagreement exists +within an OTU (such instances are rare and can reflect sequence misclassification +within the greengenes database). + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 beta_diversity.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,120 @@ + + + (pairwise sample dissimilarity) + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 beta_diversity_through_plots.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity_through_plots.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,132 @@ + + + and generate PCoA plots + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 beta_diversity_through_plots_html_generation.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity_through_plots_html_generation.py Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,56 @@ +#!/usr/bin/env python +# -*- coding: utf-8 -*- + +import sys +import os +import argparse +import re + + +def generate_index_html(dir_list, args): + with open('index.html','w') as index_html_file: + index_html_file.write('\n') + index_html_file.write('\tPCoA beta diversity results\n') + index_html_file.write('\t\n') + index_html_file.write('\t\twww.qiime.org\n') + index_html_file.write('\t\t

\n') + index_html_file.write('\t\t\tBeta diversity metrics\n') + index_html_file.write('\t\t\t

\n') + index_html_file.write('\t\t

\n') + index_html_file.write('\t\n') + index_html_file.write('\n') + +def build_html(args): + os.mkdir(args.html_dir) + + dir_list = [ name for name in os.listdir(args.data_directory) if + os.path.isdir(os.path.join(args.data_directory, name)) ] + + generate_index_html(dir_list, args) + + os.system('cp -r index.html ' + args.html_dir) + + for directory in dir_list: + os.system('cp -r ' + args.data_directory + '/' + directory + ' ' + + args.html_dir) + + os.system('mv ' + args.html_dir + '/index.html ' + args.html_file) + +if __name__ == '__main__': + parser = argparse.ArgumentParser() + parser.add_argument('--data_directory', required=True) + parser.add_argument('--html_file', required=True) + parser.add_argument('--html_dir', required=True) + args = parser.parse_args() + + build_html(args) diff -r 000000000000 -r c1bd0c560018 beta_diversity_through_plots_wrapper.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/beta_diversity_through_plots_wrapper.py Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,124 @@ +#!/usr/bin/env python + +# create a html output for beta_diverity_through_plots.py +# to replace beta_diversity_through_plot_html.py in qiime 1.8 +# Leroi Laura laura.leroi@ifremer.fr + +import optparse +import os +import sys +import subprocess + +# Create html template +def html_maker(metrics): + + tag="" + plots = metrics.split(',') + for i in range(0, len(plots)): + tag = tag+"""\t\t\n""" % (plots[i], plots[i]) + template_html= """ + + + + + + + + + +
+
+ + + + """ % (tag) + return template_html + + +def html_matrix_maker(metrics): + + tag="" + plots = metrics.split(',') + for i in range(0, len(plots)): + tag = tag+"""\t\t%s_dm
\n""" % (plots[i], plots[i]) + + template_html= """ + + + +
+%s + + """ % (tag) + return template_html + + +def main(): + + #On importe l environnemnent envqiime + pipe = subprocess.Popen(". /home12/caparmor/bioinfo/GALAXY_DEV/ourenv/envqiime; python -c 'import os; print \"newenv = %r\" % os.environ'", stdout=subprocess.PIPE, shell=True) + exec(pipe.communicate()[0]) + os.environ.update(newenv) + + #New parser + parser = optparse.OptionParser() + #beta diversity options + parser.add_option('--metrics',dest='metrics') + parser.add_option('--biom',dest='biom') + parser.add_option('--mapping',dest='mapping') + parser.add_option('--tree',dest='tree') + parser.add_option('--depth',dest='depth') + parser.add_option('--masterhtml',dest='masterhtml') + parser.add_option('--outputfolder',dest='outputfolder') + parser.add_option('--matrixhtml',dest='matrixhtml') + + (options,args) = parser.parse_args() + + #New hmtl template + master = open('./master.html', 'w') + master.write(html_maker(options.metrics)) + master.close() + #On lance le fichier avec les options precisees pour l'affichage html + os.system("mv master.html %s" % options.masterhtml) + + #parameter file, which specifies changes to the default behavior + parameters = open('./parameters.txt','w') + parameters.write("beta_diversity:metrics %s" % options.metrics) + parameters.close() + + if options.depth: + if options.tree : + os.system("beta_diversity_through_plots.py -i %s -m %s -t %s -e %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.tree, options.depth, options.outputfolder)) + else : + os.system("beta_diversity_through_plots.py -i %s -m %s -e %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.depth, options.outputfolder)) + else: + if options.tree : + os.system("beta_diversity_through_plots.py -i %s -m %s -t %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.tree, options.outputfolder)) + else : + os.system("beta_diversity_through_plots.py -i %s -m %s -o %s -p ./parameters.txt >& file_log.txt" % (options.biom, options.mapping, options.outputfolder)) + + os.system("mkdir %s/txt" % (options.outputfolder)) + os.system("mv %s/*_dm.txt %s/txt" %(options.outputfolder,options.outputfolder)) + os.system("compress_path.py -i %s/txt -o %s" % (options.outputfolder,options.matrixhtml)) + +if __name__=="__main__": + main() + diff -r 000000000000 -r c1bd0c560018 compare_categories.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/compare_categories.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,177 @@ + + + Analyzes statistical significance of sample groupings using + distance matrices + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 core_diversity_analyses.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/core_diversity_analyses.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,143 @@ + + + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 count_seqs.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/count_seqs.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,61 @@ + + + + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. +]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 filter_alignment.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_alignment.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,102 @@ + + Filter sequence alignment by removing highly variable + regions + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 filter_fasta.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_fasta.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,102 @@ + + This script can be applied to remove sequences from a fasta or + fastq file based on input criteria. + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 filter_otus_from_otu_table.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_otus_from_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,80 @@ + + + Filter OTUs from an OTU table based on their observation counts + or identifier. + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 filter_samples_from_otu_table.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_samples_from_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,85 @@ + + + Filters samples from an OTU table on the basis of the number of + observations in that sample, or on the basis of sample metadata. Mapping + file can also be filtered to the resulting set of sample ids. + + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 filter_taxa_from_otu_table.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/filter_taxa_from_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,65 @@ + + Filter taxa from an OTU table + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 jackknifed_beta_diversity.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/jackknifed_beta_diversity.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,121 @@ + + + and building jackknifed PCoA plots + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 jackknifed_beta_diversity_through_plots_html_generation.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/jackknifed_beta_diversity_through_plots_html_generation.py Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,92 @@ +#!/usr/bin/env python +# -*- coding: utf-8 -*- + +import sys +import os +import argparse +import re + + +def generate_index_html(dir_list, args): + with open('index.html','w') as index_html_file: + index_html_file.write('\n') + index_html_file.write('\tJackknifed beta diversity results\n') + index_html_file.write('\t\n') + index_html_file.write('\t\twww.qiime.org\n') + index_html_file.write('\t\t

\n') + index_html_file.write('\t\t\tBeta diversity metrics\n') + index_html_file.write('\t\t\t

\n') + index_html_file.write('\t\t

\n') + index_html_file.write('\t\n') + index_html_file.write('\n') + +def build_html(args): + os.mkdir(args.html_dir) + + dir_list = [ name for name in os.listdir(args.data_directory) if + os.path.isdir(os.path.join(args.data_directory, name)) ] + + generate_index_html(dir_list, args) + + os.system('cp -r index.html ' + args.html_dir) + + for directory in dir_list: + os.system('cp -r ' + args.data_directory + '/' + directory + ' ' + + args.html_dir) + + os.system('mv ' + args.html_dir + '/index.html ' + args.html_file) + +if __name__ == '__main__': + parser = argparse.ArgumentParser() + parser.add_argument('--data_directory', required=True) + parser.add_argument('--html_file', required=True) + parser.add_argument('--html_dir', required=True) + args = parser.parse_args() + + build_html(args) diff -r 000000000000 -r c1bd0c560018 macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,11 @@ + + + + + qiime + + + + 10.1038/nmeth.f.303 + + diff -r 000000000000 -r c1bd0c560018 make_otu_heatmap.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/make_otu_heatmap.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,167 @@ + + Plot heatmap of OTU table + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 make_otu_table.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/make_otu_table.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,65 @@ + + + Make OTU table + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 make_phylogeny.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/make_phylogeny.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,75 @@ + + + + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 multiple_rarefactions.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/multiple_rarefactions.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,75 @@ + + on an otu table + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 pick_open_reference_otus.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pick_open_reference_otus.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,295 @@ + + + + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + suppress_taxonomy_assignment is False + + + suppress_align_and_tree is False + + + + suppress_align_and_tree is False + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. +]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 pick_otus.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pick_otus.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,412 @@ + + + OTU picking + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + ]]> + + + + + 10.1093/bioinformatics/btv231 + 10.1093/bioinformatics/btq461 + 10.1093/bioinformatics/bts611 + 10.7287/peerj.preprints.386v1/supp-1 + + diff -r 000000000000 -r c1bd0c560018 pick_rep_set.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pick_rep_set.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,64 @@ + + Pick representative set of sequences + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + _rep_set.fasta by default, or can be specified via the "-o" parameter. +]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 plot_taxa_summary.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plot_taxa_summary.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,194 @@ + + based on taxonomy assignment + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 'bar' in chart_type + + + 'pie' in chart_type + + + 'area' in chart_type + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 split_libraries.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/split_libraries.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,285 @@ + + according to barcodes specified in mapping file + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + record_qual_scores is True + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 split_libraries_fastq.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/split_libraries_fastq.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,239 @@ + + to performs demultiplexing of Fastq sequence data + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + store_qual_scores is True + + + store_demultiplexed_fastq is True + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_. +]]> + + + + + + + diff -r 000000000000 -r c1bd0c560018 summarize_taxa.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_taxa.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,186 @@ + + and store results in a new table or appended to an existing + mapping file + + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + "1" in level and suppress_classic_table_output is False + + + + "2" in level and suppress_classic_table_output is False + + + + "3" in level and suppress_classic_table_output is False + + + + "4" in level and suppress_classic_table_output is False + + + + "5" in level and suppress_classic_table_output is False + + + + "6" in level and suppress_classic_table_output is False + + + + "7" in level and suppress_classic_table_output is False + + + + + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + diff -r 000000000000 -r c1bd0c560018 summarize_taxa_through_plots.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_taxa_through_plots.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,140 @@ + + summaries and plots + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + "2" in level and suppress_classic_table_output is False + + + + "3" in level and suppress_classic_table_output is False + + + + "4" in level and suppress_classic_table_output is False + + + + "5" in level and suppress_classic_table_output is False + + + + "6" in level and suppress_classic_table_output is False + + + + 'bar' is chart_type + + + + 'area' is chart_type + + + + + + + + + `_. +]]> + + + + + + + diff -r 000000000000 -r c1bd0c560018 test-data/barcodes.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/barcodes.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1000 @@ +@HWI-EAS440_0386:1:23:17547:1423#0/1 +ATGCAGCTCAGT ++ +hhhhhhhhhhhg +@HWI-EAS440_0386:1:23:14818:1533#0/1 +CCCCTCAGCGGC ++ +ccc_c^_a[[JN +@HWI-EAS440_0386:1:23:14401:1629#0/1 +GACGAGTCAGTC ++ +ffdfcfffffcf +@HWI-EAS440_0386:1:23:15259:1649#0/1 +AGCAGTCGCGAT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:13748:2482#0/1 +AGCACACCTACA ++ +ffffaffddffc +@HWI-EAS440_0386:1:23:6532:3028#0/1 +GAGAGAATGATC ++ +ghhhhhhhhhhh +@HWI-EAS440_0386:1:23:8677:3027#0/1 +CACAGTGGACGT ++ +eggggggggggg +@HWI-EAS440_0386:1:23:5678:3052#0/1 +ATAGCTCCATAC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:11889:3171#0/1 +ACGTTAGCACAC ++ +hhhhhhfhhhhh +@HWI-EAS440_0386:1:23:2112:3374#0/1 +GAGAGAATGATC ++ +eddaabddddcf +@HWI-EAS440_0386:1:23:5900:3393#0/1 +AGGTGTGATCGC ++ +fffefhhhhggh +@HWI-EAS440_0386:1:23:15468:3455#0/1 +GAGAATACGTGA ++ +ehghghfhhggg +@HWI-EAS440_0386:1:23:4405:3490#0/1 +ACGCGCAGATAC ++ +fffffaffffaf +@HWI-EAS440_0386:1:23:4674:3593#0/1 +ACATTCAGCGCA ++ +fggggggggggg +@HWI-EAS440_0386:1:23:15912:3842#0/1 +ATCAGGCGTGTG ++ +ccggggcgggff +@HWI-EAS440_0386:1:23:15732:4249#0/1 +CTCATGTACAGT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:17444:4527#0/1 +AACGCACGCTAG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:23:10725:4873#0/1 +AACGCACGCTAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:11691:4945#0/1 +ATCTTAGACTGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:4723:5217#0/1 +CTCGCACATATA ++ +\acaYW^^aYYV +@HWI-EAS440_0386:1:23:13608:5705#0/1 +GATCGTCCAGAT ++ +chhhdhhhgheh +@HWI-EAS440_0386:1:23:5195:5979#0/1 +CAGCAGCACATA ++ +ggggggggggg_ +@HWI-EAS440_0386:1:23:1372:6057#0/1 +AGCTCTCAGAGG ++ +\aab`_ccc^ff +@HWI-EAS440_0386:1:23:2305:6385#0/1 +CGTATCTGCGAA ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:23:17333:6563#0/1 +CCAGTGTATGCA ++ +ggffgggggegg +@HWI-EAS440_0386:1:23:1091:6583#0/1 +CGATGCACCAGA ++ +c^ca_\\_]``a +@HWI-EAS440_0386:1:23:16169:6630#0/1 +CAACTATCAGCT ++ +hhhhhfhhhhhh +@HWI-EAS440_0386:1:23:14554:7030#0/1 +AGTTAGTGCGTC ++ +hhhhhghhhhhh +@HWI-EAS440_0386:1:23:14518:7351#0/1 +CTCGCACATATA ++ +gfgggggggggg +@HWI-EAS440_0386:1:23:17440:7360#0/1 +GCTGCTGCAATA ++ +ggggfggggggf +@HWI-EAS440_0386:1:23:11290:7687#0/1 +ATCGTACAACTC ++ +hhhfhhhhhhhh +@HWI-EAS440_0386:1:23:1465:8235#0/1 +CACATCTAACAC ++ +cc^ccccccccc +@HWI-EAS440_0386:1:23:3176:8324#0/1 +ACTGATCCTAGT ++ +add^dcda^ddd +@HWI-EAS440_0386:1:23:12535:8807#0/1 +GAAGCTACTGTC ++ +hhfhhhhhhhhh +@HWI-EAS440_0386:1:23:14562:8883#0/1 +CGAGTTGTAGCG ++ +fhhhhhhhhhhh +@HWI-EAS440_0386:1:23:5451:9226#0/1 +AGATACACGCGC ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:23:19204:9448#0/1 +GATCGCAGGTGT ++ +cccccc_ccTWU +@HWI-EAS440_0386:1:23:13161:10184#0/1 +CTACATCTAAGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:19192:10519#0/1 +CATCGTATCAAC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:17370:10963#0/1 +GCTCAGTGCAGA ++ +gffggggfgggg +@HWI-EAS440_0386:1:23:8386:11532#0/1 +CAGCTAGAACGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:10215:11663#0/1 +AGATCGGCTCGA ++ +ffffffffffcW +@HWI-EAS440_0386:1:23:15454:11726#0/1 +ACGTACTCAGTG ++ +ggggggggggff +@HWI-EAS440_0386:1:23:6589:11781#0/1 +AGTACGCTCGAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:3256:12101#0/1 +ATGCCTGAGCAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:5860:12157#0/1 +ACATGTCACGTG ++ +hhhghhhhhhhh +@HWI-EAS440_0386:1:23:8148:12187#0/1 +AACTGTGCGTAC ++ +hhghhhhhhhhh +@HWI-EAS440_0386:1:23:14399:12333#0/1 +ACATGATCGTTC ++ +ZTPQFILLJJKK +@HWI-EAS440_0386:1:23:6966:12378#0/1 +CATACCAGTAGC ++ +\aa_aaa^aa\^ +@HWI-EAS440_0386:1:23:5056:12637#0/1 +CGCATGAGGATC ++ +gggggggggggg +@HWI-EAS440_0386:1:23:2628:12653#0/1 +ACGCTCATGGAT ++ +cccccccccccc +@HWI-EAS440_0386:1:23:3303:12826#0/1 +AGGTGTGATCGC ++ +hffhhhhhhhhh +@HWI-EAS440_0386:1:23:11685:12866#0/1 +CTCCACATGAGA ++ +hhhhghhhhchh +@HWI-EAS440_0386:1:23:3932:12883#0/1 +CTACTGATATCG ++ +aZ]]]\Sa^a]_ +@HWI-EAS440_0386:1:23:3535:13218#0/1 +ACGACGTCTTAG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:23:6909:13409#0/1 +AGTTCAGACGCT ++ +gggggfgggggg +@HWI-EAS440_0386:1:23:6191:13528#0/1 +GCTCAGTGCAGA ++ +ggggggeggggg +@HWI-EAS440_0386:1:23:17158:13561#0/1 +AATCGTGACTCG ++ +fffffafcccdd +@HWI-EAS440_0386:1:23:19363:13593#0/1 +CGGCGATGTACA ++ +hhhhhhhghahh +@HWI-EAS440_0386:1:23:10747:13717#0/1 +CCTCTCGTGATC ++ +ffcffdad\d\\ +@HWI-EAS440_0386:1:23:11158:13762#0/1 +AGCAGCACTTGT ++ +fc_ff_cdffff +@HWI-EAS440_0386:1:23:8303:13856#0/1 +CATAGCGAGTTC ++ +gggggfgaggff +@HWI-EAS440_0386:1:23:19671:14034#0/1 +CTGACACGACAG ++ +ggggggggdgcf +@HWI-EAS440_0386:1:23:3839:14047#0/1 +ATACACGTGGCG ++ +_\ffad[Waadd +@HWI-EAS440_0386:1:23:10766:14550#0/1 +CGCACATGTTAT ++ +hhhghghhhghh +@HWI-EAS440_0386:1:23:8747:14696#0/1 +GATCAGAAGATG ++ +fhhhghhhhhhg +@HWI-EAS440_0386:1:23:10687:14858#0/1 +CGACTTATGTGT ++ +ffffffffcfff +@HWI-EAS440_0386:1:23:11909:14865#0/1 +ATGGCAGCTCTA ++ +fffffffddfcW +@HWI-EAS440_0386:1:23:18778:15286#0/1 +ATTATCGTGCAC ++ +ffffdb`]_`]] +@HWI-EAS440_0386:1:23:1172:15688#0/1 +CGCGTAACTGTA ++ +ZFPFYSM\MK_a +@HWI-EAS440_0386:1:23:10432:15754#0/1 +AGATCTCTGCAT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:23:15265:16137#0/1 +CGATAGATCTTC ++ +hhhhhhhhghhh +@HWI-EAS440_0386:1:23:3853:16425#0/1 +GCTCAGTGCAGA ++ +gggggggggggg +@HWI-EAS440_0386:1:23:4837:16715#0/1 +CAGCGGTGACAT ++ +hhfhhhhhhhhh +@HWI-EAS440_0386:1:23:15530:16858#0/1 +GAGCTGGCTGAT ++ +ffffWfffffcf +@HWI-EAS440_0386:1:23:10039:17071#0/1 +GCATCGTCAACA ++ +hhhhhhghhhgh +@HWI-EAS440_0386:1:23:12821:17733#0/1 +ACCAGCGACTAG ++ +gggggggggggg +@HWI-EAS440_0386:1:23:6118:18575#0/1 +ATACGTCTTCGA ++ +cffdfdfffff_ +@HWI-EAS440_0386:1:23:5450:18847#0/1 +CTGAGCAGAGTC ++ +hhhhhghhhfhh +@HWI-EAS440_0386:1:23:2535:18871#0/1 +CAGGTGCTACTA ++ +hfgghhghhhhh +@HWI-EAS440_0386:1:23:4582:18950#0/1 +GCTAAGAGAGTA ++ +hhhhhhdhhhgh +@HWI-EAS440_0386:1:23:15559:19608#0/1 +ATACTATTGCGC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:23:9787:19631#0/1 +CGTGACAATGTC ++ +ggggaggggggg +@HWI-EAS440_0386:1:24:17851:2016#0/1 +CTACGCGTCTCT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:12302:2593#0/1 +CTATCAGTGTAC ++ +WPQFQQJSRRTV +@HWI-EAS440_0386:1:24:8488:2754#0/1 +CACATTGTGAGC ++ +\\W\\VZYYUaZ +@HWI-EAS440_0386:1:24:6114:3818#0/1 +ATAATCTCGTCG ++ +ehhhhhhhhhhg +@HWI-EAS440_0386:1:24:13084:4634#0/1 +CGCATGAGGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:9705:4722#0/1 +AGTAGTATCCTC ++ +hhghhhhhhhgh +@HWI-EAS440_0386:1:24:6985:4749#0/1 +CTTAGCACATCA ++ +gggggfgggggg +@HWI-EAS440_0386:1:24:2227:6089#0/1 +ATGCCTGAGCAG ++ +gggggggfdggg +@HWI-EAS440_0386:1:24:7654:6279#0/1 +ACCGCAGAGTCA ++ +gggggggggegg +@HWI-EAS440_0386:1:24:11532:6376#0/1 +GCTGCTGCAATA ++ +dggggggfggga +@HWI-EAS440_0386:1:24:6005:6667#0/1 +CTATCAGTGTAC ++ +fdhchhhgffhh +@HWI-EAS440_0386:1:24:9083:6728#0/1 +AGTTAGTGCGTC ++ +fggggggggggg +@HWI-EAS440_0386:1:24:9947:7672#0/1 +ATACGTCTTCGA ++ +fffffggggggg +@HWI-EAS440_0386:1:24:5510:8037#0/1 +GCATGTGCATGT ++ +fhghghhhhhfh +@HWI-EAS440_0386:1:24:5505:8369#0/1 +CTCAGTATGCAG ++ +hhhhhhfhhhhh +@HWI-EAS440_0386:1:24:4692:8430#0/1 +CTCGAGAGTACG ++ +hhhhghfhgdhh +@HWI-EAS440_0386:1:24:7038:10072#0/1 +CATAGACGTTCG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:18470:10081#0/1 +GCTAAGAGAGTA ++ +hhhhhhhhhhgh +@HWI-EAS440_0386:1:24:15353:10428#0/1 +GGACGCTCGACG ++ +gggggggggggg +@HWI-EAS440_0386:1:24:5578:10837#0/1 +AGACTGCGTACT ++ +hhhhhhghfhhh +@HWI-EAS440_0386:1:24:8169:11098#0/1 +CCAGTGTATGCA ++ +a^^\Y^]a]`ff +@HWI-EAS440_0386:1:24:15875:11235#0/1 +GCACGACAACAC ++ +hhhhdhhhghhh +@HWI-EAS440_0386:1:24:6113:13259#0/1 +ACTGTGACTTCA ++ +chhhehdhhhhc +@HWI-EAS440_0386:1:24:14722:13923#0/1 +GATCGTCCAGAT ++ +ggggggggfggg +@HWI-EAS440_0386:1:24:9379:14151#0/1 +ACGAGTGCTATC ++ +hhhghhhhhhgh +@HWI-EAS440_0386:1:24:3831:14326#0/1 +CTGTGACATTGT ++ +^]dcccffafff +@HWI-EAS440_0386:1:24:12505:14386#0/1 +GCTATCACGAGT ++ +ggggggggggfa +@HWI-EAS440_0386:1:24:2610:14604#0/1 +AGCGACTGTGCA ++ +gggggggfgggg +@HWI-EAS440_0386:1:24:3820:14809#0/1 +ATTATCGTGCAC ++ +hhhhhghhdhhh +@HWI-EAS440_0386:1:24:15451:14907#0/1 +GATCAGAAGATG ++ +fffddffff_fa +@HWI-EAS440_0386:1:24:18011:14951#0/1 +AGCACACCTACA ++ +gggggggggggg +@HWI-EAS440_0386:1:24:11603:15033#0/1 +CTACTGATATCG ++ +ggggggggggfc +@HWI-EAS440_0386:1:24:1920:15296#0/1 +ACGACGTCTTAG ++ +ggggdggggggg +@HWI-EAS440_0386:1:24:5025:15918#0/1 +CTGACACGACAG ++ +^c_ffggggggg +@HWI-EAS440_0386:1:24:3203:16220#0/1 +GCCAGAGTCGTA ++ +hhhhhghhhhhh +@HWI-EAS440_0386:1:24:4996:16249#0/1 +GACTGCATCTTA ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:14480:16254#0/1 +GACTTCAGTGTG ++ +hhhhhhghhhhh +@HWI-EAS440_0386:1:24:19188:16384#0/1 +ACATCACTTAGC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:17774:16420#0/1 +ACGGTGAGTGTC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:24:15793:17037#0/1 +CGATCGAGTGTT ++ +gggggfffcfff +@HWI-EAS440_0386:1:24:15499:17067#0/1 +CCATACATAGCT ++ +gggggggggggg +@HWI-EAS440_0386:1:24:18506:17629#0/1 +ATACACGTGGCG ++ +ffffdfffc]d` +@HWI-EAS440_0386:1:24:3796:17704#0/1 +CGAGTTGTAGCG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:6373:18472#0/1 +AGATCGGCTCGA ++ +ffffffffffa[ +@HWI-EAS440_0386:1:24:16235:18710#0/1 +CAGTACGATCTT ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:24:5656:19365#0/1 +CATCTGTAGCGA ++ +fggeggggcaff +@HWI-EAS440_0386:1:24:12256:19375#0/1 +CATATCGCAGTT ++ +hhfhhhghhhhh +@HWI-EAS440_0386:1:24:11025:19521#0/1 +ATACTCACTCAG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:24:9307:19872#0/1 +ACACTGTTCATG ++ +hhhhhhehchhg +@HWI-EAS440_0386:1:25:1361:1248#0/1 +CCTCTCGTGATC ++ +aZ\\_VTV\TTa +@HWI-EAS440_0386:1:25:5646:1376#0/1 +ACATCACTTAGC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:2107:1388#0/1 +AGCTGACTAGTC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:1851:1463#0/1 +ATGGCAGCTCTA ++ +a^ffaff[f_aY +@HWI-EAS440_0386:1:25:3982:1463#0/1 +ATACAGAGCTCC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:4646:1592#0/1 +AGTGCGATGCGT ++ +aggggg_[ggfa +@HWI-EAS440_0386:1:25:4380:1628#0/1 +CCTCTCGTGATC ++ +hhghhhghhggh +@HWI-EAS440_0386:1:25:1510:1668#0/1 +CTGAGATACGCG ++ +hhhhghhghhhf +@HWI-EAS440_0386:1:25:3929:1715#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhgh +@HWI-EAS440_0386:1:25:4840:1834#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:25:5558:1851#0/1 +AGCTGACTAGTC ++ +ff_ccc^^a[MR +@HWI-EAS440_0386:1:25:13850:1850#0/1 +ACACTGTTCATG ++ +hhhhhhhhhhfh +@HWI-EAS440_0386:1:25:2639:1894#0/1 +CCGACTGAGATG ++ +gggggggfgggg +@HWI-EAS440_0386:1:25:6139:2018#0/1 +AGCTGACTAGTC ++ +hhghhgggcahh +@HWI-EAS440_0386:1:25:3109:2059#0/1 +CTGAGATACGCG ++ +hhhhghhhhhhh +@HWI-EAS440_0386:1:25:6937:2060#0/1 +ATGGCAGCTCTA ++ +cYcYc\Z]LO\S +@HWI-EAS440_0386:1:25:7669:2080#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:25:7091:2092#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhfhh +@HWI-EAS440_0386:1:25:3922:2110#0/1 +ATGGCAGCTCTA ++ +ggcgggg]caf_ +@HWI-EAS440_0386:1:25:3806:2202#0/1 +AGTGCGATGCGT ++ +ffdffggggggg +@HWI-EAS440_0386:1:25:6540:2228#0/1 +ACTACGTGTGGT ++ +hhhhhhfhhhfh +@HWI-EAS440_0386:1:25:10019:2223#0/1 +CGACAGCTGACA ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:2668:2254#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:19646:2248#0/1 +ACAGACCACTCA ++ +hhghhhhhhhhh +@HWI-EAS440_0386:1:25:1802:2336#0/1 +AGTCACATCACT ++ +ghhhhhhhhhhh +@HWI-EAS440_0386:1:25:5054:2329#0/1 +ACTACGTGTGGT ++ +ghgghhchhhhh +@HWI-EAS440_0386:1:25:8482:2380#0/1 +CTGAGATACGCG ++ +_ffffafffdfg +@HWI-EAS440_0386:1:25:15658:2382#0/1 +GAGACAGCTTGC ++ +_ccaacacccac +@HWI-EAS440_0386:1:25:8261:2388#0/1 +CTGAGATACGCG ++ +hhhhhhhhhhhf +@HWI-EAS440_0386:1:25:9735:2401#0/1 +AGTGCGATGCGT ++ +hhhhhhhh_hhh +@HWI-EAS440_0386:1:25:8948:2437#0/1 +AGCTGACTAGTC ++ +gggggeggggcg +@HWI-EAS440_0386:1:25:12681:2438#0/1 +ATGGCAGCTCTA ++ +ggggggfcedfd +@HWI-EAS440_0386:1:25:12916:2459#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhfh +@HWI-EAS440_0386:1:25:7336:2484#0/1 +AGCTGACTAGTC ++ +bba]^addaacf +@HWI-EAS440_0386:1:25:1489:2530#0/1 +AAGAGATGTCGA ++ +eff]caffffd_ +@HWI-EAS440_0386:1:25:9578:2531#0/1 +ACTACGTGTGGT ++ +hhghhhghhgde +@HWI-EAS440_0386:1:25:9159:2552#0/1 +CCGACTGAGATG ++ +fffcfcadfaaf +@HWI-EAS440_0386:1:25:11396:2559#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhfh +@HWI-EAS440_0386:1:25:1760:2573#0/1 +CCTCTCGTGATC ++ +gggggggggadf +@HWI-EAS440_0386:1:25:14068:2621#0/1 +CCTCTCGTGATC ++ +hhhhhhhhfhhh +@HWI-EAS440_0386:1:25:8159:2639#0/1 +ACTACGTGTGGT ++ +hhgghghhfafe +@HWI-EAS440_0386:1:25:13134:2667#0/1 +ACACACTATGGC ++ +hhhhhhhhghhh +@HWI-EAS440_0386:1:25:11175:2690#0/1 +ATGGCAGCTCTA ++ +fffdffff[]]\ +@HWI-EAS440_0386:1:25:8576:2709#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:6943:2731#0/1 +GACGCTAGTTCA ++ +ggggggagggfc +@HWI-EAS440_0386:1:25:1850:2741#0/1 +AGCTGACTAGTC ++ +f^ffffcfdffd +@HWI-EAS440_0386:1:25:10428:2744#0/1 +AGTGCGATGCGT ++ +hhhhhhhhhhgh +@HWI-EAS440_0386:1:25:12398:2774#0/1 +ACACACTATGGC ++ +hhhhhhhghhgh +@HWI-EAS440_0386:1:25:14110:2805#0/1 +ACTACGTGTGGT ++ +ghhhhhhhhhhh +@HWI-EAS440_0386:1:25:14127:2819#0/1 +ATGGCAGCTCTA ++ +gggggggfaaf_ +@HWI-EAS440_0386:1:25:1968:2841#0/1 +ACACACTATGGC ++ +ffff_fffffgg +@HWI-EAS440_0386:1:25:10866:2839#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:11767:2838#0/1 +AGTGCGATGCGT ++ +hgghhhghhghg +@HWI-EAS440_0386:1:25:4402:2852#0/1 +AGTGCGATGCGT ++ +gghhhhghghhh +@HWI-EAS440_0386:1:25:1421:2878#0/1 +AGCTGACTAGTC ++ +fdfffgggcgff +@HWI-EAS440_0386:1:25:11319:2883#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:3648:2910#0/1 +AGTGCGATGCGT ++ +ghhhhhhhhhhg +@HWI-EAS440_0386:1:25:6586:2930#0/1 +CCTCTCGTGATC ++ +hhhfhhhgghhh +@HWI-EAS440_0386:1:25:8471:2931#0/1 +AGCTGACTAGTC ++ +hhhghhghhhhh +@HWI-EAS440_0386:1:25:14208:2940#0/1 +ACTACGTGTGGT ++ +hhhhhhhhhghg +@HWI-EAS440_0386:1:25:2027:2951#0/1 +AGCTGACTAGTC ++ +gcggggeggfdf +@HWI-EAS440_0386:1:25:13175:2941#0/1 +GCTGCTGCAATA ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:13489:2951#0/1 +AGCTGACTAGTC ++ +hhhhfhhhhhhh +@HWI-EAS440_0386:1:25:6132:2959#0/1 +ACTACGTGTGGT ++ +a`aY]ada^e\L +@HWI-EAS440_0386:1:25:2692:2971#0/1 +ACACACTATGGC ++ +hhghhhhhhfhh +@HWI-EAS440_0386:1:25:1831:2983#0/1 +ACACACTATGGC ++ +ggggcggacg^[ +@HWI-EAS440_0386:1:25:6335:2989#0/1 +CCTCTCGTGATC ++ +afdeff\ffaca +@HWI-EAS440_0386:1:25:10162:3028#0/1 +ATGGCAGCTCTA ++ +fffffdfRc]^[ +@HWI-EAS440_0386:1:25:4348:3042#0/1 +ATGGCAGCTCTA ++ +ffa_fffa_d\J +@HWI-EAS440_0386:1:25:5972:3048#0/1 +CTGAGATACGCG ++ +hhhhhfhhhghh +@HWI-EAS440_0386:1:25:9861:3050#0/1 +ATGGCAGCTCTA ++ +gggggggcfcea +@HWI-EAS440_0386:1:25:10090:3050#0/1 +CTGAGATACGCG ++ +ghhhghhhghhh +@HWI-EAS440_0386:1:25:3139:3077#0/1 +AGCTGACTAGTC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:9110:3081#0/1 +AGTGCGATGCGT ++ +hhhhhhghhhhh +@HWI-EAS440_0386:1:25:17734:3100#0/1 +ACTACGTGTGGT ++ +ggggggggggfc +@HWI-EAS440_0386:1:25:8423:3141#0/1 +ACACACTATGGC ++ +hhhhhhhhhghh +@HWI-EAS440_0386:1:25:14009:3149#0/1 +AGCTGACTAGTC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:3100:3158#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:4738:3152#0/1 +AGTGCGATGCGT ++ +ggggggggggfg +@HWI-EAS440_0386:1:25:4197:3165#0/1 +CGAGAGTTACGC ++ +hhhhehhhhhhh +@HWI-EAS440_0386:1:25:9072:3171#0/1 +CCGACTGAGATG ++ +gggg_ffdffgg +@HWI-EAS440_0386:1:25:15751:3166#0/1 +AGTGCGATGCGT ++ +fcccffdfffgg +@HWI-EAS440_0386:1:25:3473:3225#0/1 +CTGAGATACGCG ++ +gfgfggfggggf +@HWI-EAS440_0386:1:25:4755:3229#0/1 +CTGAGATACGCG ++ +hhhhghhhhghh +@HWI-EAS440_0386:1:25:3032:3237#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:1630:3249#0/1 +CTGAGATACGCG ++ +hhhhhghhhhhh +@HWI-EAS440_0386:1:25:4476:3251#0/1 +AGCTGACTAGTC ++ +hghhhhhhhhhh +@HWI-EAS440_0386:1:25:3312:3259#0/1 +ACTACGTGTGGT ++ +hhhhhhhhghhe +@HWI-EAS440_0386:1:25:3550:3291#0/1 +ACACACTATGGC ++ +hhhhhhhhghhg +@HWI-EAS440_0386:1:25:5478:3298#0/1 +CCGACTGAGATG ++ +hhhhhhhhhfhh +@HWI-EAS440_0386:1:25:15346:3296#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:3468:3322#0/1 +ACTACGTGTGGT ++ +fhdfhhhhgdfe +@HWI-EAS440_0386:1:25:4199:3321#0/1 +CCTCTCGTGATC ++ +hhhhhhghhhhh +@HWI-EAS440_0386:1:25:8568:3330#0/1 +ATGGCAGCTCTA ++ +gggggggf_fdd +@HWI-EAS440_0386:1:25:12050:3335#0/1 +ACACACTATGGC ++ +gggggggggggg +@HWI-EAS440_0386:1:25:19075:3342#0/1 +CAGTACGATCTT ++ +hfhfehgghhhh +@HWI-EAS440_0386:1:25:4994:3354#0/1 +ATGGCAGCTCTA ++ +gggggggffef[ +@HWI-EAS440_0386:1:25:12091:3381#0/1 +ATGGCAGCTCTA ++ +ffffffffcaa_ +@HWI-EAS440_0386:1:25:8594:3395#0/1 +AGTGCGATGCGT ++ +hhhhhhhhhhhf +@HWI-EAS440_0386:1:25:2052:3400#0/1 +CCTCTCGTGATC ++ +ggggggggfgfg +@HWI-EAS440_0386:1:25:11776:3405#0/1 +ACTACGTGTGGT ++ +hhhhfhhfhe[f +@HWI-EAS440_0386:1:25:4155:3413#0/1 +AGTGCGATGCGT ++ +fffffggggggg +@HWI-EAS440_0386:1:25:11052:3431#0/1 +ACTACGTGTGGT ++ +hhhhhhghhhhe +@HWI-EAS440_0386:1:25:4122:3435#0/1 +ATGGCAGCTCTA ++ +c_cYc`WKZP\Y +@HWI-EAS440_0386:1:25:9796:3444#0/1 +AGTGCGATGCGT ++ +hhhchhhhhhhh +@HWI-EAS440_0386:1:25:2235:3462#0/1 +AGCTGACTAGTC ++ +fcaffffcfcgg +@HWI-EAS440_0386:1:25:14529:3458#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:6708:3509#0/1 +ACACACTATGGC ++ +ffffdfffffcf +@HWI-EAS440_0386:1:25:13919:3507#0/1 +CCTCTCGTGATC ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:10705:3535#0/1 +ACACACTATGGC ++ +hhhfhhghgfgh +@HWI-EAS440_0386:1:25:7788:3570#0/1 +CTGAGATACGCG ++ +hhaghghhhdhh +@HWI-EAS440_0386:1:25:2273:3577#0/1 +ACTACGTGTGGT ++ +ffffffdfffe_ +@HWI-EAS440_0386:1:25:14850:3657#0/1 +CCGACTGAGATG ++ +aaYc^aaYZZYT +@HWI-EAS440_0386:1:25:11581:3662#0/1 +ACTACGTGTGGT ++ +hhhhhhhhhhhg +@HWI-EAS440_0386:1:25:16965:3660#0/1 +ACTACGTGTGGT ++ +hhhhhhhhhhhg +@HWI-EAS440_0386:1:25:8197:3691#0/1 +CTGAGATACGCG ++ +hhhhhhhhhhhh +@HWI-EAS440_0386:1:25:11617:3701#0/1 +AGCTGACTAGTC ++ +`\__\W\\\Y\^ +@HWI-EAS440_0386:1:25:15484:3705#0/1 +CCGACTGAGATG ++ +hhhhhhhhhhhh diff -r 000000000000 -r c1bd0c560018 test-data/forward_reads.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/forward_reads.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1000 @@ +@HWI-EAS440_0386:1:23:17547:1423#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGATTGGTGTG ++ +hhhdHddddddddfehhfhhhghggfhhhfhhgggfhhgfgdfcfhehfdgfhggfggfggffgddfgdffdgdaagaaddcbdccc]a^ad__a]_____ba_`a`__^__\]^OWZR\Z\\WYTZ_U^BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:14818:1533#0/1 +CCCCNCAGCGGCAAAAATTAAAATTTTTACCGCTTCGGCGTTATAGCCTCACACTCAATCTTTTATCACGAAGTCATGATTGAATCGCGAGTGGTCGGCAGATTGCGATAAACGGGCACATTAAATTTAAACTGATGATTCCACTGCAACAA ++ +US[QCQSZPHNYIa[^aaccaac[]accBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:14401:1629#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGGTTGGGGG ++ +fffbF`bbWZZ]Zggggfgcgggggddggdgggggdbgdddgbgeggg`ffdggeabba`aaad]]]d\]`]`[]]aWaYa\aa`a`_````_^]^^^]]]X]_``____``BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15259:1649#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGAGTTTGGGGG ++ +hhhdHcdd^bbbahhhhhhfhhhhhghhhhhhhhhghhhgehchfhhhgghghfhhhgehgaffaecfdggdh\bafadddddgddfdbc^]a_\^_a``\X`^_`]`aaabcaYbY_^^X]^ZY^Z^a`_^aBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:13748:2482#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGGATTCGTGGTG ++ +fffbF^bbS[T[Uggggggg__gffddfggeggggaagfeefbfaaff_cfacfeceddgggegdgdgaggddcddd`a_aad`ddadd`daaWY]]Yddda_]_]]a_Y___X\_]YaY]\]WYNV]\]_BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6532:3028#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGAGTCTTGGGG ++ +hhhfHdffacdcchhhhghfhhhhfehhhchhhehghhgghhgehghhffghhhgghghddgggfhfgdhhdea`Wf^dddddbgagcdedgcfddbcddaddcefb_addddd_daaaaaaa`ca`aX^Z\X_^^^^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8677:3027#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGCCGGCACATGATACTGTGAGACTAGCGTAACGGAGGGGGAACCGGAATTCTTGGTG ++ +ggg`Fbbbdeeeegggggggggggfgggggggggggggggggfgggggggggfgggggggf]gfccfcfbfbdged]ddda_dedaa]aZc__BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5678:3052#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTGGGG ++ +hhheHeeeaaabbhghhhhhhhhhghhhhghhhhhfhghhghhghhhhdhehhfghhaehhgghddhhhhhadffffdb]Z__Z^_`^daadda`c`Z\T[\\]\RJ]YS[\X_a]]P_XM\T\^\^^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11889:3171#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTATGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +hhheHddeeeeddhghhhhhhhhhhchhhhhhhhhfhfdddffhgghhghgehhegghhhhffhffhhg_edaecdcc[dabaadddaadaaeY\WQX^]]^^``RZZ___a__aa_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2112:3374#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGTGG ++ +fffdGcddZZ___ggggggggggggggggfggggggfgdggggeggggeggggggeggdggeggggggdggdgcaaf\^_b_b_gdedd_eedR^Z^^`dcRb_P__R^\[]^c`cbadaaab^da^aBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5900:3393#0/1 +TACGNATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTG ++ +hhheHeeea\aaaghhhhhhhhhhhhhhhhghhhhfhgggghhhhhhfcghhhhgfhdgahddfbddfcfca]daed]cfcdcb]bbb^]_^bUZRYW___^_abaV_^SYYW````]^_[O_^```Z\RS^BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15468:3455#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGGGGTGGGGGGGATTCGGGGGG ++ +hhhdHaddddedehhhhghhhhhhhchhhghhhghghhgfghhddfdhggfhfhehghhfhgghdhehghhfgaaaf_cacaadaadbb`bcaacabcdbecc`ddaaa_aabacaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4405:3490#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACGGCTTTTGAAACTGTCCTGCTTGATTTCAGGAGAGGTAAGCGGAATTCCTAGTG ++ +fffdGdcdbabbbggggggggggggggggggggggggggggggfggggggggggfggWffcfdgdggggggdfdeWadggededacgegdddgcd__da_c`XebdadedaedaJaaaW``_RZW^[W]Q^BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4674:3593#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGAACCGGAAATCTCGGTG ++ +gggUDW_Y[a^^aggggggggagfffffcffeeccffabbbbcfefffdeggdggeggggddda_cf_bY__`bbbaa_cccaaL^aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15912:3842#0/1 +AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTAGGTGGAATTCCCGGTG ++ +fff\Faaa`babbggggggggggggggggggggggggggggggggggfggggggg`ggcdfdegegggfafdfgggdgggggfa_eggaddbdaaf]`fecddgdddb[``a\aaa\`bddbdacb]caacBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15732:4249#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGTGGGGGG ++ +hhhdHdee`bbaahhhhhhghhhfddffhehhhhhehhhdhhhaeddhghfheh_gghghedehggdhghggfaacfaedf_fabddaaa_fab_```decdhcegdW[_b`bdaeaa^b]W`[`]``BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:17444:4527#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGATGTGAAAGGCTGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCCGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG ++ +hhheHdedfffffhghhhhhhhhhfffhhhghhhhfhfggghhghhghgggfghhhfggfdffgachh]ca_aaabdd_cdead]_aa_]]_baaa_a_a_]_\YJWZX\U\Z^^^U_a``^a\aBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10725:4873#0/1 +TACGNAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG ++ +gggdGbcdb^b`\fffffgggggggdggggggggggggeeefffcfdfgggggggggggcgfga[_cfdfeb_`_bbdddeefdbdccdgaggggdafcddbgdeaeaaaaabdgdda]]b_bccecacaaBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11691:4945#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATACGTGGTG ++ +hhhbHbbbcdddchhhhhhhhhhhgghhhhhhhhhhhhhgchhghhhhhhhfhhghhhhhhgghghfhghhdhdcdhcgghghfbggdedghedcdah]dbaacdgddcaeee]dd_ac_``_TT[T[Z_XZV_^W^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4723:5217#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACCGTAGGGGTGGGGGGAATTTGTAGGG ++ +NRRKD\ZQVUVSTcccccccbccccccccc_cccccaccccccccccc_ccc_bcc[[cccccccc[caccccc^ccP]Z`Z_ccccccccccHQGYVa_acccccYc_ccccI[Va]\\^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:13608:5705#0/1 +TACGNAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGCCTGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGAACTGCAGCGCGAACTGGCTGGCTTGAGGACCAGCAACGTGGGCGGAATTCATGGTG ++ +hhhbH`ba_Z_a]^ffffhgd]hffdffhhhhghhbhhdedfffbfef_acfcdddadgaaeeaRcaa```^`___ca_aaabcbcabdccdcacaaa_Zb__\__[___Z]_aabBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5195:5979#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGATGTGGGGGG ++ +gggbF^bbSa\_\gggggffaefffdafgggggggdggeedfafa[cfdgeggggggg]ggddgdgegdggegabad`dbbbcdddbdd`adT]TY]_]_Z]`aae`c]]bYd]Z`WWaa_]aBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:1372:6057#0/1 +TACGNAGGTGGCGAGCGNTGTCCGGAATTATTGGGCGTAAAGGGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +fffcGcccZY]]Y`abaEccaccfffdfffWffffffcfaffafffffcfffcafbdfc_ccefccfc^W^^abbcacYYX^Vccaccdc`b]^ZP^X^M^Z^`W]W]a_aZa]^MX]]]M]W`_XW`BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2305:6385#0/1 +TACGNAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTGTGTTTGAGGTAGGCGGAATGCGTGGTG ++ 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+dggdGdcdadddcgggggggggggggggggggggggggggggggggggggggggfggggggggfggfgg_gdgggdffegggffbggecddddababdgfffdff`da_acfa]a]`]c^c]daabaaa[c_aYY]]_T`XQNU\TUU]_[a +@HWI-EAS440_0386:1:23:17440:7360#0/1 +TACGNATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGCGGCAGTGCAAGTCAGGAGTGAAAACTTGGGGCTCAACCCCAAGACTGCTTTTGAAACTGTACAGCTAGAGTGTAGGAAGGGCAAGCGGAATTCCTTGTG ++ +gggdGdddddccdggggggggggggfgggggggggggggggggadddffdfffgggffegggdggggg_ddb_ebddeebgcgfggff_dePdaaca_dddgdff[daadccaa]aaaaaKaa_aT\a\Z[^RY\\\\]_BBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11290:7687#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGGACCGGAATTCTCGGTG ++ +hhhdFeee^aaaaghhhhfdb_dfddcfghfhhhhfhhhfhhccffffhhhhcfhhhhhhggefdff_fefefdddbe[ddedc_Zaa^[^^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:1465:8235#0/1 +TACANAGGTCTCAAGCGNTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGGGAGACTTGAGTAATGGAGGGGGAGCCGGAGTTCTCGTGG ++ +cccLD]YZVZ]]]aaaaDcccccccccccccccccacccccccccccccccacccccccccccccccccccccccccbTTP]]cBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:3176:8324#0/1 +TACGNATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGATGTGAAAGGCTGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCCGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG ++ +ddd]D`__Z^Y]UfffffffffffffffffWfffffafffffffdffffffccfffff^ZO^^dad]d^dd^dcfcf[IZ`Z^ffcfededa`^WZZWcfaaaXTWYH^^^^SWM^X]cbbacacb[c[H^BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:12535:8807#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGGATTGTGGTGT ++ 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+TACGNATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCAGGCGGTTTAATAAGTCGAATGTTAAAGATCGGGGCTCAACCCCGTAAAGCATTGGAAACTGATAAACTTGAGTAGTGGAGAGGAAAGTGGAATTCCTAGTGT ++ +b^^aD^aaccccccccccccccccccccccccccccccccccccaccccccccccccccccccccccacccccacccc`cbcbc`aaacaa_ac_a__a^accca[_[X__Z_NRMJT__Z_VX[X[aY_BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:13161:10184#0/1 +TACGNAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATCTGCAAGTCAGTAGTGGAATCCCAGGGCTTAACCCTGGAACTGCTATTGAAACTGTGGGTCTTGAGTGAGGTAGAGGCAGGGGGGATTGCCGGGG ++ +hhhaHddccdccchhhhhhhhhhhhfehhhhhhhhehhhhhhhhghdhghhhhhhhhhhgchghdhfcddddf_acffeghheefghdgagbhhgddhegdggehhaa_]`b_dedab`[`__adaabY]^\_Z^Z^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:19192:10519#0/1 +TACGNAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGGCTTTTAAGTCTGACGTGAAAATGCGGGGCTTAACCCCGTATGGCGTTGGCTACTGGAAGTCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +dhh[Ga\\daacchhhhhhdhhhhhhhhhhhhhfhghhchdhhhhhhhhgfhhhhhghhhhcchhhhhhhhhhdhehhghchhhghdh_fcWcfaccf``d`]UPIWMYZSYW]\L[Q\[Z[Va_a_[^_BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:17370:10963#0/1 +AACGNAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTAGGTGGAATTCCCGGTG ++ +fggdGeeedccccggggggggggggggggggggggggggggggggggeggg[gggg]daafcffedfcaafffeddaefdddcddcdegggcffWdafddedcgg]g]caff[aac[cccf]c_aR_a[cc[ca]aacV^BBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8386:11532#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGGGGAGTTCGTGGTG ++ +hhhbH^ba\aaaaefhhhhghhhhhefhhehhhhhghhhghggehgggeghdghhe`gfghgggdgghfggfgfdcgdffgfccfbfeddcfcfccdfdfcdfaee]a_]\\_]^Y^\b__]]\_aa\_\a_aBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10215:11663#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGGGGGGGG ++ +fffbF`bb`bbbaffffffffffffefffffccfffffffffffefffefffdffefffefedfdeefedddf[aTc_ddedecdddddddcaddaadccY_cbddad]aaacZQ^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15454:11726#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCCCATGATACTGCGAGACTAGAGTAATGGAGGGGGGAGCGGAATTCTGGGGG ++ +fffbFbbb`abbagfgggggggggggggggggggggggggcgeggggfe[fffgggfeba`b`fcaccfacafccacaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6589:11781#0/1 +TACGNAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTATGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCATGGCTAGAGTTTGTCAGAGGAGGGTGGAATTGCGCGTG ++ +gggaFaaa^\\aafffffggcggffd_fgggggffefffcfffggggfgggggggggggggfafcfdf[dfdcddddfbfdeddddfbcc_ff_b]`abb`[bgegd_cddac_ggdddcc_[]``]]X]`Z]^VZ\\W_\OIRTEWWV^X^ +@HWI-EAS440_0386:1:23:3256:12101#0/1 +TACGNATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGGAATTCCTAGTG ++ +gggZE_X\\a\aaggggggggggggggggggggffefefddfffdfffggggggaggggggg]fdfefdegdgbacg]bddbdd`aaV_^aa^TXSOU__\_aaaa\_`aaa^U]\_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5860:12157#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTGGGGGGG ++ +hhhdHadd\```ahfhhhhghghhgffhhghhhfhfhhggfhhdafdfeehbhhgfghfhgffhffdhfggfgddcgcfefbgaffdcdaaedb_aabdfcdcdefcddae[c`c_`\acaabcdccfaa^^^^FX^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8148:12187#0/1 +TACGNAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +hhheHdee^bb``ghhhgfadefhgdahhghhhhhghfhgghhghhfhgggfdgffgggfhfegddgggadagdabeecdaeab`dacaaaaad__bd__b_aa_\_\SY\[YaaZaa_a\_\a][^aXXFX\Z\Z^\_BBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:14399:12333#0/1 +TACGNAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCGCAGTGT ++ +]]^ZE^^^^bbbbggggggggggggggggggggggggggggggggggggggggggdggggggggddedcacacbbfbdddfcdb`ddddadQda_Rcaa]aaac`[___a`[_^ca^abb]b]aW\]aX_W`BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6966:12378#0/1 +AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +^[[YE\Z[\\YV\ffffffdfffffffffffffffffffffffffffdfff[]dd_]dcc[dcffcdfcfffffdf[c^^a[affdfffff[b]a^^^fffQad^Ya[a^adW`````cccQfZZIP^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5056:12637#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGGAATCGTGGGG ++ +gggeGdeebbbbagggggggggggggggfdffcffeffgggggggggggggggggfggggggfdcddfdfcdfbbafbdefcdgfefdcb_eabbabdceacfadda_bad_dbad_a]`]`Z\aa_`_d___U_]Y_BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2628:12653#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGGGACTAGAGTAATGGAGGGGGGACCGGACTTTTCGGGG ++ +cccLD[[]]bbbacccccccccccccccccccccccccccccacccccccccccccccccccbc_cc_aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:3303:12826#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAACCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGAACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +hhhbH`bbbbbb`hhhhhhhhhhhhhghhhhhhhhehdgdghghghhhgghghhfhghgghefgddheeggagdcdgefdcfcd[ce]aaaaadcaX`aaadacacWa^Z^]Zaa_aR^]^VZZZ\\^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:11685:12866#0/1 +TACGNAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGGG ++ +hhhbHbbacccdchhhhhhahhhhhhhhhhfhhhhehghhhhfghhhheghhfhhfhhhghh_edeeheghdef[dfcafaf_cdeeebdbfcb^`cb_``__ddccaffca__aaaada_a__^``[TOYPZaaZ`_U`__^BBBBBBBBB +@HWI-EAS440_0386:1:23:3932:12883#0/1 +AACGNAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTGGGTGGAATTTCCGGTG ++ 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+AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +fggdGadd`deeeggggggfgggggffggggggggfgggggggggggegggggggggeggggggfgggeagdggfbggeaggbdbddaaa_dbec_cddddddfdccddd^fccce````badaa_aaa_aV_`Z]]]Q\]PW\\\\V\\T_ +@HWI-EAS440_0386:1:23:17158:13561#0/1 +TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGAGACTAGGGTAATGGAGGGGGGAGCGGGCTTCTCGGTG ++ +eee_D]``\aaaagggggggggggggggcgggggegggggggaggggggggggfgggggggffdddfafacacaYaaaad[bb`ZZ]\a_bY_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:19363:13593#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTATAATTGCAGTTGATACTGGCGACCTGGAGTGCAACAGAGGCAGGCGGAATTCGTGGTG ++ 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+ddddGdddTVY]ZfffffffffffffffffffffdfcfffdfffffffffffcffcfQ^[dadfce]]a]adW````]cacce[cf_cccaaO^U^Z^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10766:14550#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGGG ++ +hhheHdeedeedeghhhhhhhhhhhghhhghhghhghhhghhhhghghghhghhhhhfhhhhghfhghghhfhddchbhghahfffffdadhddb_aededdgdffWbaadddZ__S^_^_^_\a_aaadb\a_\U_^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:8747:14696#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGCGGGATTCGTGGTG ++ +hgheH`dd_acaafhhhghhhghhhdhhhdhhhggehhffdgcffhfhffghggfg`hgdedefdffgfgfddaaae`dacbcdccdaaaagcaaaad`a]]a]aa__a__Yaabaa]\_YM\S^[Z^\ZYL[^T[Y^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10687:14858#0/1 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+ddd^E_a\addccgggggggggggfcgggggggggggggfggggggggfeffcggggggggfffdfdfgggggdddgdgggdgdYdcdbaadaddddgffdcgaggad_a]aaccfcc]cc_a_\__a_\aTaWS^BBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:1172:15688#0/1 +TACGNAGGATCCGAGCGNTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTGCAGTTGAGGCAGGGGGGAGTTGGGGTG ++ +W_WQF^NN]QN]NcZROEQ`U^NXX\XX[f[Qbe_f__d[^V``dTdVffYfb`efddafY_daff^[feefcfWccffffffafafdfffbfa\dddfaccffffW_ccddcKaaa[Y\aK_T\`W_V`bZ^a^Vb^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10432:15754#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG ++ +hhhbH`bbaddcchhhhhhghhhhhghhhhhhhhhghhhgehhhdhdhddfdfhhddhhhhgghdeehghhggdccheeggdgadada``^d`aa]aaddbbe_ddad]^b`badaaa_dd_bad_]da`_\_^\[_^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15265:16137#0/1 +TACGNAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTGGTCGCGTCTGTCGTGAAATCCTCTGGCTTAACTGGGGGCTTGCGGTGGGTACGGGCCGGCTTGGGGGCGGGAGGGGGGGAGTGGAACCCTGGGG ++ +ghhaHcdc_aacchhhhhfcfffhfhahhhhhhhgfhhghhhgghggghhhghghhghhgccdfcaffdaffddhdhd]ddffa_cc_[[```hea]e^^_d_]a^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:3853:16425#0/1 +AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +gggdGccddeeedgggggggggggggcggggggggdgeeedffegggggggggegegggdgfcc_ffcccaacaddgeeggddadagd_abcddaaaadc_gaaebaaaaac_dcbb``bb_aaa_^\YXZLUa\\__BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4837:16715#0/1 +TACGNAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGGACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTG ++ +hhhdHaddcaccdhghhhhhfghhhhdhhhhhhhghhhffffafffffhhh]hgggghcdhhchfhheghhfdgffeggghgadddggde]fc_`Qcb_acaadce^ddaaadddaba_b__bedddd__aOa__`BBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15530:16858#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGTG ++ +fffcGccc`abbbffffffffffffafffffccfffffffffffffffeefefffcefdfdfffcccfbffcfcccfcfdddcdcedb]bbebacdd[bcaacda[addbdc_WZ`ZZab_]bYZ`XaYaaBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:10039:17071#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGGTTGTGGTG ++ +hhhdHcdddeeeehhhhhhhhhhhgfhhhhhhhhhghhhgehhhfhhhggghhhhghhghggfhegdhghhfgdcbeahdgdcffffbdaagdcccacbae[aaddaba]dccccdccaaa_aQ][Z^\`^Z]_\_aBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:12821:17733#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAATCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCGGAGGGGTAAGCGGAAGTCCTAGTG ++ +gggeGdeeacddcggggggcgggggeggggcggffbfbcacffgggggcgff]fe_fffc]]ac_c]ccfc_abbefd^`\^\aaac_aa_afb`W``aaa[a_ca\]]]`]]acK_\\W\N]_d_aa]ST_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:6118:18575#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGTGGGG ++ +dffbFbbaUZZ^Zgggggg_gggggg[gggggggggagggfgggggfgafffafd^bbff_fffed]f^`dbdcccf_d_b^dfffddgfgcbceeeeggeWgf]cW[cccf[X^W`_accaceeaac]`b]]c_`a`BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:5450:18847#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG ++ +hhh`Hbbb_aaaahhhhhhhhhhhfhhhhdhhhhhhhhhhhgghghfhhhfhhhhghhhhghhhghhfehhghfgcfdhgggggfhhgggdg_addbdceedecggdg_dedcacaaa^[[WSPZZWW[X[XX^ZW^^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:2535:18871#0/1 +TACGNAGGATACGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCAGGTGGTCTTGCAAGTCAGTGGTGAAAAGCTGAGGCTCAACCTCAGCCTTGCCGTTGAAACTGTAAGACTTGAGAGTACATGATGTGGGCGGAATGCGTCGTG ++ +hhhaHdbacddcchfhhhhchhhhhahhhhhhhhhhfhhhdhhhhhghhhhhghhhedhggghhhfafdfhhf^_^ccdabbdhcghheghhh]fcfchhdeaccfcW`cffcb^K[^bbb]d_bW`R^d`R`fccaBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:4582:18950#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG ++ +hhhbH]b`^``^`ghhhfhhhfhhhdhhhfhhhhhfhhgffggffgfhdffeggdghgfgefdfcffgadddeaaad_dddddadadacc`dcdccddaa`cd_ad_aUWQUT``_\]_a_\\\__\_ZZ^BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:15559:19608#0/1 +TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGGGGGG ++ +hfhaG_aacdddchhhhhhhhhhhfghhhhhhhhhhhhgghhhhghhhgghghhhhhhhhhhghgfghhhhfhdechdhegdefcfff_abebgbdaeada_dadd_e_]a`cWXX[P[[WVVNXJOVZTYTS_\Y__BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:23:9787:19631#0/1 +TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTAGAGCTAGAGTGCTGGAGAGGCAAGCGGGATTCCTAGTG ++ +gggeGeeea`ab`ggggggggggggggggggggfggggggcgggggggfggggddggggeggggfgdgfdgdgdddaadaaaaaV_b_aaaLa\__\O]_]\GZUOWO\S]\S\U\ZZ_\_^_\aBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:17851:2016#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTTTCGTGGG ++ +hhhccdcFcdddchhhhhhghhhhhghhhfhhhhhghhhhhhhfghhhgghhhhggghfhegfhgb]hdhhdhac]daWTTNEXWWTS_^`b^_abbdddcadcddRd_adbbcacaaadaaa^^\^`__a\\^^V^^\^^BBBBBBBBBBB +@HWI-EAS440_0386:1:24:12302:2593#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGGNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGTATTTGTGGTG ++ +aaa\\a_DRZV^Zcccc_cccccccccccccccccc]cccccccIcccZ]cRZ``VMZ^a]a]cccccbcccccaac_WJQLDVVTLK[[[[SLYTYYccccbccc`cZV[][PKSUW[ZXZ[X_BBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:8488:2754#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTTAAATTGCAGTTGGTACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATTGCGGTG ++ +]YZZZ_^DQZUSV^c]accccccccccccccccccccccccccccccccccccccYLcaccccc[POccccccaccQ[UMQGD^XZWTaaL]UIFUHT[]^]^NFFFSY\\XWH[ZJTcc]ccccbcBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:6114:3818#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATAGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG ++ +hhhddacF^bb`ahhhhhhhhhhhfghhhhhhhhhghhggghhhghghgghghgghhhhhhhghgffhhhhdccdafc]\\[H^\][[ccaedefcdfffdbfccdcdaabdbacda`_b^_a`aa]caa[]__Y[aBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:13084:4634#0/1 +TACGTATNGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGNGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGGGTGTCGGGGGGGGAAGGTGTATTCCTATGG ++ +hhhdaacFa[a^ahhhhhhhhhhhhghhhhhhhghghgcccffhghhgfghhgghhhhhhhggdaeffdhedebcdecZZZ^FZVYVY_^`Lb`^\\[daaaaddb_b^Q^]^X[^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:9705:4722#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGGAGTCGTGGGG ++ +ghheedeFacdacghhhhhfhhhhhahhhhhhhhhfhhgggghgghhhgegehhg_hcggehggfgbhfggfhcddhd]]^^H\^\\\dadebddddccfdefaadcdbabaadab]_]^WY^BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:6985:4749#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGCTTCGGGGGG ++ +gggaa^aEZ```_fffffgagggggggggcgggggggggegggggfgggfgggggegggfggggdggfefeeade_gd[[ZYF\Z[ZZdd]dcgacgddcdcedgeadaacacWca_`ca_aaadd_aaa]aBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:2227:6089#0/1 +TACGTAGNGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCAAGCAACTCAGAAGTGAAAGGCTGGNGCTCAACCCCGGGACTGCTTTTGAAACTGTTTGACTAGAGTGCTGGAGAGGTAAGCGGAATTCCTAGTG ++ +gggdaacGdcccaggggggggggggdgggggggggfgfgegggggggggfgeggegggdfeddK]_^^bdeaddcfce\Z\`F\XX\ZcddadddQaaaaccaaaa___aba]b\_]_b_W]a_baaBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:7654:6279#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGAGTTGTGGGGG ++ +gggdeaeF^`b``geggegggggggfgggggggggfgggggffgfgggfbgdggdffgfgbfffcfbgdgdacaaada\][^G\[W[[a_[daaaabbccccd`cd`_a^`aaaa__W_a]]_^]___BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:11532:6376#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGCCTTGAGTGCAGTTGAGGCAGGGGGGGTGTGGGGGG ++ +gggddccF`bbbaggggggggggggdgggcgggfggggggggggggggfefefgggcgggggggggggggggfecbfe]]]]F\[Z[Zddcfeaeddedbddedggcfeabcbdbeaa`c`\_JZSWZ]__BBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:6005:6667#0/1 +TACGTAGNTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGNGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGA ++ +hhhedeeHcdddchhhhhhghhhghhghhhhhhhchhhhhhhahghhhhhhehfhhhghhhhhhfhghhggegddghh`a`dFa][^^dgcdggbeegdaacdedcVa`]a^`cgWd]dd[d]ddb_a_][`PZ__^^Wa_\a_ZYaBBBBB +@HWI-EAS440_0386:1:24:9083:6728#0/1 +TACGGAANGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGANGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG ++ +gggdeedGdcddcggggggggggggdgggfgggfffffdffffdcfcfgggggggagfggggggcgggggddabeefd[Z[JFNRRRRcbddafaa_dcaaR[R^Y^Q\\\[^M\[[X^\^W\QRKUQaa_aaBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:9947:7672#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACCGGGTGTCTTGAGTACAGTAGAGGAAGGCGGGAGTGTGGGGG ++ +ggg\^\_DTWY_Wcfffcgagagffddffdfccffacfdddfdf_edffggfgbeabdeddadgdd`e]`b_b`\_a[OMMMEVRTNMaaaa]IRU]S___\_``b]_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:5510:8037#0/1 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+hhhaadaFdccdcgfhhhhhhhhhfghghhhhhfhhhhfhfhffhhhghhhhehfhghhhhhhhgghhhfhhhgeehe`]^`H^^^\\ghdhhhaeeeggefhfghe_^_Z]_`b]`__f`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:7038:10072#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGCTTTGGGGGG ++ +hhhbb`bE\aaaaadfffhhhhhhhchhhhhhhdhhhhhgghhhghhhgehghhhghhghhhfhggfhghhfhdcbhc]\][H[ZYZXbbafddbbaddd_adade_d__cc]_aacYc\``_V\RS\\_^^^BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:18470:10081#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG ++ +hhhddccFdeeeehhhhhhfhhhhhhhhhghhhghhhhhhfghgghhhdhfhchghhhhghghhhhhhghhgedcdhd\Z[ZGZZUYVecdhegfffgfgdchdggd_cbddabaa_bdaaabaddad\_c^_BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:15353:10428#0/1 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+fffaYaaEIQZ]Wffffffffffffcdffffffffcffffcfcfffbfbeffcffaacf_`c]facac]d`]`aaaca]U[]FZ[YXYddd_daO_a_a]a]_ZMPZ^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:15875:11235#0/1 +TACGGAGNATCCGAGCGATATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTTGGGGGG ++ +hhhffdfG``b`a^``\K[XRX[ghbhhgghhhhhchhggehggfchhddffahdahhfghhdgfgghdhhdeaa_ca][\[H\\[\[adagcaadcdcccadaaa`d`^\_^a_aW__a_\_VXVY[\V\QY__\a]X^BBBBBBBBBBBB +@HWI-EAS440_0386:1:24:6113:13259#0/1 +TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGATCGGGGGG ++ +hhheedeFacdachghhhhghhhhhghhhghhhhhfhhhghhggghghfghgfhhghhfhhhhhfffhehgeeeddha_^^^H][\[[ddahcadaddadcceadgdcbab]cbbbdacf`]cac][bad_a_a\_aaBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:24:14722:13923#0/1 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+AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +ghhhhhhhhhhhhhhhhhhhhhhhhhchhhRhhfffffhhhhh_ghhfhhgfhhhhhghhghhhghhcdffdffgghghghhfcehae[caccahcgghhfffehedg``bd`dhchXhhebeeddde[aa[c\\^^^[_BBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8159:2639#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTGGGGGTG ++ +hhhhhhhhhhhhhhhhhghfhhhhhfhhhghhhhhfhgggggghghghcdhfbhegghgdhgdgdgdhdhgdhacaeaebcccfdfddcccfbacaac`adadcfcd`dababaadaa_daVaa_aaaU^BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:13134:2667#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGCGCGGCTGGCGGGCAGGAGAGGTACGTGGAATTCCTCGTG ++ +hhhhhhhhhhdhfhhhhhhhhhhghhhhhhhhhfhdhghhhhhgghhhhhhgfggggheffddhaghhadgdefffafdfdfafcdacd`aadaa\ZRRYTMY__^U^\O\^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11175:2690#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGGGGG ++ +bbbfafffdfffffffdffffffffcfffffdcffeffffffffeccfeffdfffadfdfcfffef]feffefeddfdede_ddddedadcecbc_aadcd]dcbeadcade]Z`]Y]``W]]Z``BBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8576:2709#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATGGTGGGG ++ +hhhhhhhhhhhhehghhhhfhhhhhehhhghhhhhhhhhhchghhhghfhhghhhhhhhhegghfgfhghfdcaaafafgegfbfgfdaddhddaddbddgbgadeaaa_aaab]d_a^__^_\__aaZ]_^]_^]`BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6943:2731#0/1 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGGG ++ +fffgggggggggggggggggggggggggggggegfggggggggggggggggggggfgggfggegggggfggdgcddcd_bddacegddfagafb_dfbddddbfdffaaddad``da```dWda``]bT\_[]__BBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1850:2741#0/1 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCGGAATTCACGGTG ++ +cffdaddd^aKa^fffffffffffffffff]ffcfffffffffffffffffffff`]]f_]f[d^add_ffcffcfacfda]ccfcfeefcWeaa^^]fcfca`_`cZ```dT^aZZS`ccaaa_a_cPM^W^^LWYYZ^BBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10428:2744#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGTGTG ++ +hhhhhhhhhhhhggghhhhhhhhghahhhehhhhhghhhhhhdehhehdgegghhehhhhghghdhghghhehbdbhagghedgcdfdbaagdcbacebddagaadb]a\aaabdac`_aa]a]aa_ba\^_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:12398:2774#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG ++ +hhhhhhhhghhhchhhhhhhhhhhhehhhghhhhhghhhhghhhhhhhhhhhhhdhhhhhhhfhggdhghgghbdghgghh_dgdgedfcfgfdeddeffadgadd[e__acccbacaccaaa]aaadada__]_\_aBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14110:2805#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGCGTGTCGGAGGGGTAAGTGGAATTCCTAGTG ++ +hhhhghghdfffdhhhhhhhhhhhhfhhhghhhdfafccccfffhhhadghhegghhehhfcab_]dbR_^]_fdff__cac_dWbb\aacad`bKRW\\YZ\_^KR\XL[Z[^`^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14127:2819#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTCGGGTGG ++ +ffffffffgggggggggggggggfgggggggggfgggggggggfdggggggggggfggggggfdaddfdffefbbaf_ddecdddfcddaagdcdaddffadfa`dac_`gcb^bd_^acaY]UW[\\\aBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1968:2841#0/1 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCTAGAGTGTGTGAGAGGATCGTGGAATTCGATGTGT ++ +ggggggggdffffggggggggggggegggggggcaffeggggaffcccggggffggaefebffeadffcfc]c]X`b`bdddaddaabca_de]caa_bRI\_``_\_a`ddabba_bBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10866:2839#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGAACCGGAAGTCTCGGTG ++ +hhhhhhhhhhhhhfhhhhhhghhhhhhghghhhhgfhhhhhhdhghhhgghhhhhhhdhhegdfcdfahehcefffafcfddbd__dda^d[]^_`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11767:2838#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACCGTAGAGGTGGGGGGGATTCGGGGGG ++ +hhhhhhhhehhhhhhhhhhhghhhgghhhghhghhghhheghheghhhgeghhcgghghhhhghdgchfhgfeccbeba_cdaedebeb]dccaaaaa```^`_aa_d_^]L_`_a^^_caL`BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4402:2852#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTGTCTTGGGGACAGGAGGGGTGGGGGGGAATTGTGGGG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhghhhhhhhhhhhhhhghhfghhhegghghfhhhhghhhhfgghghfghdbbfbeeheegcefa\__caaaaaaca^_daaa\a]\a_aaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1421:2878#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGGACATGATACTTCGAGACTAGAGTAATGGGGGGGGGACCGGGGTTTTCGGGT ++ +_ffK[b]`fffffggggggggfggfgggggggggggggggggffffffgggggggggggggggffffffffdf[ccfaafafd`Q]b_cKcccBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11319:2883#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +ghhhhhhhhhhhhchhhghhhhhhhahhhfhhhhhfhfhghehgghhhfghhhegghfcehddhdehceggfdfagbegfdfcfbcdabbaaddb]a_a]aW_aaaYaZ^^^X_a]b_]Z^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3648:2910#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCGCATGATACTGCGAGACTAGGGTAATGGAGGGGGCACCGGAATTCTGGGGG ++ +hhhaffffhhhhhhhhhehhhghhhhhhhhhhhhchhhfffffhhhghhhhhhhhhhhhhhgefdffdfdfefeebaedgggagcadbdbgddaacVa^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6586:2930#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGGAAGCGGAAGTCCGAGGG ++ +hhhhhhhhhghhhghhhhhhhhhhhhhhhhhhhhhhhghggghhghghdhhedgghhhhhhhghfhghacgdgfgggfggdfcabce]ccbaed_^aa_a_VWaaZ_\__^_\Z`\^^^^_Y]\_\aBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8471:2931#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhfhhhghhhhhghhggghhhghhhddhehhheghhhggdhfffhfhhfhffchdffgfgcdd`abbdcab_aadaacaeddf`aa_abb[[^FJ\][[ZY`O^`BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14208:2940#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGGG ++ +hhhhfhhhhhhhhffhhhhhhhhhhhhhhhhhhchhhhhhhhghggghhhgehhgghfhhhhgfdhcfehhghgedhdgfhfgffdf_ddafddacdddcfdggcfggfccbfacaa^cac_a^^`W`^_c[aVL^BBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:2027:2951#0/1 +TACGGAGGATCCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAACTCGTGGTG ++ +fffffffffffffcfcffgggggggaggggggggggggggggggfgggddf_cgggggcggggfdgaggegdgdc[fceefbeddbdd]a_fadaaaadd]be]```BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:13175:2941#0/1 +TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATAGCAAGTCAGATGTGAAAACTATGGGCTCAACCTGTAGATTGCATTTGAAACTGTTGTTCTTGAGTGAAGTAGAGGTAAGCGGAATTCCTAGTG ++ +hhhhhhhhhhhhhhhhhhhhhhhghhchhhhhhfhehehhhhhhhhhhghhhhhhhhgghhhghfhghggfghgffgcggggbefdffdabgeadeacddcaeddddaaaedadd_d_caa_aX\ZZ\^_`\`_\^``Za]WZW[\R\\_BB +@HWI-EAS440_0386:1:25:13489:2951#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGGTACTGCGAGACTAGGGTAATGGAGGGGGAACCGGGATTTTCGGGG ++ +hhhhhhhhhhhhhghhhhhhhhhghhhhghhhhghfhhhhhhchhhhhhddfhhghhhhhhgffdffdfcdbdggdgbKUW\\`ZU__aaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6132:2959#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGGAATTACTAGTG ++ +`]afffffffcffffffff_fffffffffffffffffffffffcffcfbee]^ffffffdffffffWffffcffaf[fdf[ffdcdffffddfP\^^adec]bd]bdbcdadb^]KJ^`\^^\\a_aa`[]]N\X^W^]]^BBBBBBBBBBB +@HWI-EAS440_0386:1:25:2692:2971#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG ++ +hhhhhhhhhhhhhehhhhhfhhhhhahhhehhhfhehhhgggghghhheghfdhhgghgghg]hehdhggfghab]fbedhddgfdfcddcfcabaaaccddg_[aaadabaddeddaddd_`Y^_aa[TWFQ[[[UXZ^`BBBBBBBBBBB +@HWI-EAS440_0386:1:25:1831:2983#0/1 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAGTGCCGGAGAGGTAAGCGGAATTTCTAGGG ++ +deeffffffacffggggggggggggfggggfgggggggggggggggfgafdffdf_ffffdeefdbffbbd`bcccWfZ_`___accaedgdca]Q_`dda]a``]]X]\``VV]]_^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6335:2989#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAGTAGAGGGGGGTGGAATTCGTGGGG ++ +addfffffRdadcdddddffR[fddc[dff_dcffa[fdddd`aMSaaT]_]Kdd[ddffffcfefff]ff`ca^^[PUNSTXcecfeeecaPTIXYN`````G^^ZZebbaccc_]aacaaaWT]Z`BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10162:3028#0/1 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCGGAGAGGGTGGCGGAATTCCAGGTG ++ +aaafffffccfff`b`_KZ[TWZfcffffffeffaffdffffffffdfdfdfafdfffdfWddededfeae`cbbdacaeadddd`daaacc[]`W]]`ddad_caaWca_aO]XH]]_\W_]YRYW_BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4348:3042#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGTGGGGGG ++ +[_affffffffffffffffffffffcfffffddfffffffffffffffffdffffffadfffffdffffffffafQfafcffcdfcfedfefdfeecddfcdfdecdfccacebWebaccbbccaeabT``]`]`[``BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:5972:3048#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG ++ +hhhhhghhhhhhhhhhhhhhfhhhhhghhhh_hhhchhhhhhhdghghghhghhhhheghhgfhfgdhhheghfgfcgggbfdbbcdfgcdcfdaaadcfcafbbb^`_\a__Wa_aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9861:3050#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGTTGTGGGG ++ +fffggggggggggggggggggggggggggggggggggggggggggggggggggfggggggggfggggggggdgbc_fcgaggggdffdd`bd_dbdbbaca[c\c__`]W````T_WX^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10090:3050#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATTGTGGGG ++ +hhhhhhhhfhhhgghhhhhhhhhhhdhhhdhhhhhghhgggggggghhfgdghhhghgeghbfgdedhdhgdg_\ac_cdedbaYaaababebaab\bbb_Wd_baabaaaaacWc_Z``_^^accdfca``c\]___BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3139:3077#0/1 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGCGGCAATCGGAATTCCGTGGG ++ +ggggggggcffffgggggggggggggggggfgggggggggggggfgggedggggdgggggggggggcggggffgfgfcgggefffggggggegfgfgfbddQda[`c_dddaadf`fdbdaaWP^Z^^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9110:3081#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGTGGTTGGGGGG ++ +hhhhhhhhhhhhhfhhhhhhhhghfghhhghhhhhghhfghhhhghhhghhfhhhghghhhgghfhghghhfhcbahdeggegcfgfdcadfbdcbcdbaaad_cc_a`]`^ba_a__acaaa\a_\aaaaaacVaaaBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:17734:3100#0/1 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT ++ +ffffffffcffffggggggeggggggggggggggggggggggggfgdggggggggdgedeaffgffggadfdfegadggagfggcgggfgfagbW]W^dbcddddb[abdda_XPZ\\aaYa_aa]a_NYYYZT\\]]`BBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8423:3141#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGCGGATTTGTGGTG ++ +hhhhhhhhhhhhhhhhhhhghhhhgghhhhhhhhhghhhhefggghfhfgghhhhhhghhgghhfeehgggfhbgfgfgffgdcggfceffffffca_abbac`ad_d_aaaaddae_^`]^\\a_N\^_^]Z_aa_BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14009:3149#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGGGGAATTCGTGGTG ++ +hhhhhhhhhhhhhfghhhhchhhhhfhhhehhhfhhhhhhhhbffhheacff_heehegfghgcadbdgfgfeecWebfbffffefdacdddaddfdfaa]aa_aabb`\Z^^\[^ZS]`_\^Y]_^^Y__G_BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3100:3158#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGAGTGGGGGG ++ +hhhhhhhhhhhhhhhhhhhchhhhhgfhhhhhhhhfhhhehghhghhhfhgehhghhghfgffgdg[gfgfdeab]dadddebfdfcaddbdaabb[_acaadcacac_ababccc`ca_\\_^_a\__aRa\__BBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4738:3152#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGGATAATGGAGGGGGAACCGGAAGTCTCGGTG ++ +gggaffffdgggggggggggggggfgggggggggggggffffffffffgcfggggggggggggfdffdfdfdfgcg]ggfbeffaccddabdb`Qb]_[^\]`a__]b_a]_bBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4197:3165#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGCGGAAGTCGTGGTG ++ +hhhhhhghhhhhgfhhhhhhhhhhgchhhhhhhhhhhhghhhfhhghhhhghhfff`ffcffchhhhhfhhhhfhhhbhggheghhhhchfhf_adddaeedeeeeadgddffcccf\aaaaa`gcda`_`\W^X\R^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9072:3171#0/1 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTAGGGGG ++ +ggggggggggcgeggggggggggggfgggfggggggggggggggggggffff]gggggdcfccfefffcffafddfaffWf_cdffdeded[ffddedffgfRcfaaaccfc_fecddcacaa]aaacaac_]^YZ^^\^aBBBBBBBBBBB +@HWI-EAS440_0386:1:25:15751:3166#0/1 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGGATTCACGGTG ++ +gggffffffffffgggggggggggggggggggggggggggggggcggggggggffPc[^`Z\Zfefaff[fcfccffa``cf_ggagefgdfd[^\WWccc`c__aaQYZ^_^a]]cZ`X`WbBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3473:3225#0/1 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTATGGGG ++ +gggg_ggggggggggggggfggggggggggggggggggggggggfgggfggggggggggggfdgggegdfdcfcccffeeecc`adddccbefddcab`daddddd_daadaaddddWa__Y[W\\[\a``]`_Y_W_BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4755:3229#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGCGGTG ++ +hhhhhhhhhhhhhhghhhhhhhhhhghhhhhhhhhhhghgehhghhghggghhhgghgghhhghfhgheghegdcahceedggffggdedahdeebddc]dcdaddcaddgcadedacfddccaedcdabaaaZbaaaBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3032:3237#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGCGGCATTATGGGTG ++ +hhhhhhhhhhhhhghhhhhhhhhhhghhhehhhghghhgghgghhhhhgehhfhec`fhhgeggdg_hghhfhgffhdgfgdgfgegfdddhggddd_ddddhdegadcefceffddcddaaPW`___a_WW_BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:1630:3249#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGGGGTAAGTGGAATTTCTTGCG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhghhhhhhhhhhhghfhhfhhhhfhhhfhhhghhhhhggghhhghhhghggh[gehegdhgdgffgbagaf[ccc`^_`c_W\a``^[U^``^^P[[XZQTSU[^JY_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4476:3251#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGCACCGGAACTCTCGGTG ++ +hhh]ffffhhdhhhhhhhhghhhhhhhhhhhhhhahhhhhhhhhefhhgghhhghhghhfeggfdffcfbfdfdfeddgeggf_H_^^`Z`\ZYZ[I\Y\[\^^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3312:3259#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGAATTTGGGGGG ++ +hhhhhhhYcffffefhhhhghhhhgehhhchhhhhghhhgchhhbhhhdghghhhghhhghhgedd]hhggfhfd]edddegddffffbgdgffdfcddff_fccdcfdadfcad[dadaaab___BBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3550:3291#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG ++ +hhhhhhhhhghhchhhhhhdhhhhgeghhhghhhhgehhhghhhhhhhdgfhchhchfheeahhgdchdddefdgdhaebecdfgfffgfgfd]ddcdadaaccbbccb]cbb_b^babd_aaaa]X_[]^Z_JZ[^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:5478:3298#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTGGAGGTGGGGTGGAATTGGGGGG ++ +hhhhhhhhhhhhhhhhhhhhhhhhgghhhghhhhhghhhhhgggghhhgghhfhghhhgghhhgbhghegheedd_gcgfgefffffdfddgdfgdbfddffgaaccaaWa_a__`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:15346:3296#0/1 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTCGTGGTG ++ +hhhhhhhhhhhhghhhhhhhhhQffcffhghhhhghhhhghghhdhhhhehhhhhghhhhhhfheechhhhghddchdhdeggadhbdacbdadbcdddgdaeabdaca\aac^``^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:3468:3322#0/1 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGGGTGGAATTCCGCGTG ++ +hhhhghhhfffffhghhhhgahhhghhhhhhhhhfhhgggghhhfhhchghhhhgghedhdddecbeefdfcgffdffffgfddcffddccfeWdaabcddcaad___ababaa_daaaa_\\S_^^R\\\XX_Pa_BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4199:3321#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGGAATTCCTAGTG ++ +hhhhhhhhhfhhhfhhhhhhhhhhgchhhhhhhhhghhffdffhghhhhhghdhhhdhhhgfhhghhfhfhdhgghgggfdfdb[dgbffgded[da_aad]bbda]aZN\^\ababa]aa]__a[_^[WTIXQWTVW\_aBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8568:3330#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGGGG ++ +fffgfgggggggfggggggegggggeggggggggggggggggggdgggddfcfgggggggggggfggggggggcffgfggggedgffagadgdfgfgffgfcg`dgdgaacaaaab]adddaa`d``ddad`a][_aBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:12050:3335#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGAGTTGTGGGG ++ +ggggggggdggggggggggfgggggggggcggggggggggggggggggggcggggdeggggg_gggggdggdfdddgcgdegdfgd_dgffgfb_aWcdddbdaddcWZ^\^^cabcabbbacaa[aaabdWdR\R\^BBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:19075:3342#0/1 +TACGTAGNGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTCGTACTTGAGTATCGGAGAGGTAAGTGGAATTCCTGGTG ++ +hhhdeeeHdcccdhhhhhhhhhhhhhhhhhhhhhhhhhhhhhghhhghhfhghhhhhhfhhddhbefhdfhcfdeaedaebedd__baa]bab_a[G`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4994:3354#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGGATTTGTGGTG ++ +fffgggggggggggggggggggggggggggggggggggggggfeggggffgeggggggggggggfgfgdggdgc]bdbffgg_ffbeffdfgfa_aaaddddg_aabba_a]bd[b_aadaa`cd``]\`__\Y\\^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:12091:3381#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGTG ++ +dddffffffffffffffffffffffffffffffffffffffffffffffffcffffffffffefeffffcfeffe]dfdfdedcWcfcdddafcfacecacacdd[adc__`_WaZ\X]W]J]ZZSXZ^BBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8594:3395#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGTG ++ +hhhhhghchhhhhchhhhhhhhhhfchhhdhhhhhhhheeehhhghhhgdhhhhfehhhfhhghdgdhechehfffgdhdcgbfgfggcddhddddddfff]fcdcaa]`c`c_`Z]S[\[XUVY[[\Y^BBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:2052:3400#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGACTAGAGGGCAGGAGAGGTAAGTGGCATTCCTCGGG ++ +ggggggggggggggcgggggggggggfggggggggdggffafffggggggggggfggggggfdfeefeefecedegecgddgabQadbW\^^`aaa_c__]aaaa_T_VV\\Pbb_aaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11776:3405#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTTGTGGGG ++ +fffhhhhhhhhhhhghhhhfhhhhhchhhfhhhhhhhghhhfhhfhhgdfffbhhehhhghhhhgfghgghdggdccgeahceeggedfffgf]ddadabdab_aaaa\UX\KYPWUYdadabZZ_Y_BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4155:3413#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGGACCGGAATTCTCGGTG ++ +gfgaedeeeffffcffffggggggggggggggggfgggbbbdffffffgggggggeggggggffcffafcc_fedddbbfgbfaO___\W^^[QW^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11052:3431#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGTG ++ +hhhhhhhhhhhhfhhhhghhhhhhgghhhhhhhhhghhgghhhhghhhgfgghhhgghghhhghdddhghhfhebchaggh_ggfdcc]a_cadaaadddadebdfdda[caaddada_aa[b\aV_a_ba_BBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:4122:3435#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGCCGGAGGGGTAAGTGGAATTCCTAGGG ++ +S[_]cccaccccccccccccccccccccccaccccccccccccccccccccccccc[accccccccccccccccccc[ccc]cc[cbcccc[cSWR[W]][]SaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:9796:3444#0/1 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG ++ +hhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhghghhfhhfhhghhhhhhgghahhhhhhhfhdhgfhhhdghh_hfhgecghfchggeddhfaa[cceeeedecdaacffaebddbccc_c_eaaccZaa``_cXcYY^R\[UTWTTZY[ +@HWI-EAS440_0386:1:25:2235:3462#0/1 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG ++ +gggfdfffefaffggggggebggggggggggggggcgdeeef_ddde[geggggcgeWcgaggg`fgfeec`cccebaddcc`bdadadaV]d]]H]_dcbb_a[]YTYUY]UW]O`ZX]ZY_^[]^^_aBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14529:3458#0/1 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGCCGGCACATGATACTGCGAGACTAGGGGAATGGCGGGGGAACCGGAATTCTCGGTG ++ +hhhhhhhhghhhghhhhhhegfhhfhfhahghhhchhdhhhhahhhhhggchffgggghhggfhbefbfbd_dddcdaR`_`a_NU^^^^__^`^`G^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:6708:3509#0/1 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG ++ +fffgggggggfggggggggfgggggfggggggggggggggggfgggggggggggfagggggagggggggegfgaggefdfddadgggccca_fa\^\^ddd]dacR\_``accc]aab`[`\`BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:13919:3507#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGTGGGG ++ +hhhhhghhehhhffghhghchghhfahhhdhhhggdhhgdfgggdgfhcadfahedeeddgdagdebgffgdgd_agaeceddfdccdaaagd_caacadaadcddcad]`dbcbca_aa_Yb_aa_a\O\O]^\]_BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:10705:3535#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAACTGGCCGGCTAGGGTGTCCGGGGGGGACAGGGGATTTCCTGTGT ++ +hhhhhhhhdhhhhhhhhhhhhhhhhehhhhhhhffdfeffffchhhhhhghhhhhhhhfhhhfhdhchhfheagghhcahgeg_ddbadeedh`][^`Z_ZZ```RK^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:7788:3570#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ++ +hhhhhhhghhhhghhhhhhhhhhhhhhhhhhhhghhhhhhghehhhhhgghehhhgfgghghhhgghheehbhaWacfc_ca`bedcfbddaeeebaaadada``ZW^]^```aabb_aa]]b_ca_a_V___WRZW_WX[VJ\W\G\T^X^ +@HWI-EAS440_0386:1:25:2273:3577#0/1 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGACTTCCCGGTG ++ +cfffeffeggaggffffdggdgggggaggggggee]fb`]```fcfgdgaffgffbbd[ca_W]Z``ZY^]U]YS[^`_`_`\`add```aa]a_[[a`a_a```a`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:14850:3657#0/1 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTGTGGTTGAGGTAGGCGGAATGCGTGGTG ++ +[__fffffffaffffffffffffffffffffcdfffffffffffaffffffcfffa[affffffffff][fffcfcfffdfcfffffdfed[cfccccfffafccfcYaaccc^^Y[^ffcaaZaBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11581:3662#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGGG ++ +hhhfhhhhfghhffhhhhhghhhhghghhhghhhhdhdheefffhehghhgehghgcehhhfdgdgghghffedff[gdfac]_]`c`ddd_c^`\[\_a___a_a]aaaab`__H]_[S]P]__]Z]`SY_`BBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:16965:3660#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGGG ++ +hhhhhhhhhhhhhhhhhhhghhhhhchhhhhhhhhghhghhhhhghhhgchehhhhhhhhhhghfhfhgghdheedhdhghehffgfgcgggfgfa[ddcgdeffgafdcgggabadaddc_aW___`_a___W^R^BBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:8197:3691#0/1 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGCG ++ +hhhhhhhhhhhhghhhhhhhhhhhgfhhhghhhhhhhhhgghhhhhfehhhhhhhhhhhhhgfheefhehhehfgahgfhghfgffdccacaec]a__aaa\aaca^_aYaaadddba^^`S_S^\\^BBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:11617:3701#0/1 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG ++ +\a_fcfffdfdffffffffdfffffcffffdddfffafffffffcfffffff_ddaad^^^cafffdfaffcffffab^O^[aefe]acfccfbaccccccccdffeccc`fJGQYRXca```BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HWI-EAS440_0386:1:25:15484:3705#0/1 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGGTAGGCGGAATGGATGGTG ++ +hhhhhhhhghhhhhhhhhhfhhhheehhhehhhhhghhhgdhhhghhhgghehhhhghhhggghfggeghgdhcaaefgegedgdhhdacaddcbcde\caccbac\c_acdacebb]a_aSa\_\]_\aa]W^BBBBBBBBBBBBBBBBBB diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_otu_map.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_otu_map.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,10 @@ +708680 L1S76_81 L1S140_70 L1S8_6 L1S76_31 +851865 L1S8_28 L1S8_76 L1S281_24 L1S105_1 +589071 L1S140_45 L1S208_48 L1S105_50 L1S257_5 L1S257_27 L1S257_54 L1S281_2 L1S105_16 L1S140_47 L1S57_30 L1S105_34 +531539 L1S208_60 L1S208_83 +183480 L1S208_7 L1S281_19 L1S208_57 L1S257_55 L1S281_8 +583117 L1S76_13 L1S257_63 L1S105_35 L1S8_84 L1S140_9 +589277 L1S208_15 L1S257_22 L1S140_67 L1S140_69 L1S57_52 L1S57_62 L1S281_64 L1S140_26 L1S208_58 L1S208_14 L1S140_32 L1S208_3 L1S281_12 L1S57_68 +580629 L1S105_71 L1S76_74 L1S76_61 L1S8_38 L1S105_29 +535375 L1S8_4 L1S8_23 L1S76_21 L1S8_53 L1S57_41 L1S281_78 L1S76_25 L1S76_73 L1S281_43 +363400 L1S57_77 L1S57_42 diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_otu_table.biom Binary file test-data/pick_open_reference_otus_otu_table.biom has changed diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_otu_table.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_otu_table.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,12 @@ +# Constructed from biom file +#OTU ID L1S76 L1S140 L1S8 L1S281 L1S105 L1S208 L1S257 L1S57 taxonomy +708680 2.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 +851865 0.0 0.0 2.0 1.0 1.0 0.0 0.0 0.0 +589071 0.0 2.0 0.0 1.0 3.0 1.0 3.0 1.0 +531539 0.0 0.0 0.0 0.0 0.0 2.0 0.0 0.0 +183480 0.0 0.0 0.0 2.0 0.0 2.0 1.0 0.0 +583117 1.0 1.0 1.0 0.0 1.0 0.0 1.0 0.0 +589277 0.0 4.0 0.0 2.0 0.0 4.0 1.0 3.0 +580629 2.0 0.0 1.0 0.0 2.0 0.0 0.0 0.0 +535375 3.0 0.0 3.0 2.0 0.0 0.0 0.0 1.0 +363400 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_otu_table_2.biom Binary file test-data/pick_open_reference_otus_otu_table_2.biom has changed diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_otu_table_with_taxo.biom Binary file test-data/pick_open_reference_otus_otu_table_with_taxo.biom has changed diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_otu_table_with_taxo_without_pynast_failure.biom Binary file test-data/pick_open_reference_otus_otu_table_with_taxo_without_pynast_failure.biom has changed diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_representative_sequences.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_representative_sequences.fasta Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,20 @@ +>183480 L1S208_7 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>363400 L1S57_77 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG +>531539 L1S208_60 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG +>535375 L1S8_4 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>580629 L1S105_71 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT +>583117 L1S76_13 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>589071 L1S140_45 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>589277 L1S208_15 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>708680 L1S76_81 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG +>851865 L1S8_28 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG diff -r 000000000000 -r c1bd0c560018 test-data/pick_open_reference_otus_representative_set_tree.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/pick_open_reference_otus_representative_set_tree.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1 @@ +((589071:0.01564,(580629:0.01561,589277:0.00014)0.960:0.04297)0.241:0.02059,583117:0.00014,((183480:0.16413,(851865:0.20153,(363400:0.07236,(531539:0.00867,708680:0.00659)0.893:0.08113)0.886:0.09917)0.903:0.10496)0.964:0.14514,535375:0.03431)0.597:0.00813); diff -r 000000000000 -r c1bd0c560018 test-data/split_fastq_libraries_demultiplexed_sequences.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_demultiplexed_sequences.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,344 @@ +@L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA ++ +ACCCGGGG@G?GGGGGGGGDGGGGGGGGGGGGGGGGDGGBGGGBGGGGGGGDGEGGDDG@GGGGEEEGGGGGG388GD>DDGGEE@DDDDB>FDGD>@-:74=;A*74====?BBD@ +@L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG ++ +GGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHFHBHHECCHBDHFEHAE@@BDB=B@@A2D(5553DAAAAE@EEA>;)9;==A>7B@=@78;:82?C<><9?<>>>98=@ +@L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA ++ +IIIGIIIIGIIIIIGIIHIDIHIHHF@IIFIIIIIGIIIHIHGHBIIIGHHGIFEEEFHHHDEEDG>DBEFBDB@BB=EEBBBEGEDDBA>D@@@@BCBB;BD@@>:@@>B@?=1@@@3<9767=6>;;? +@L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG ++ +IIIIIGIIIIIIIGIIIIIIIIIIIFIIGEGGDIIIIIFFHIIGDGEGD>GDGIIEIGIIFH>GBDDGFGGEGBB2DBDDGBDEBEBB8?B>A@D=38=AC>?>@@?DD@>=?=478;+;BE8>B +@L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIHIIIIIIHEEIIEIIIIIIGGHIHEIIHDFCIGHHHCEDBIEIEEHBBEBBEIHGGGGGDHGGEEGDG?<2A5DBBBBEBEBBB@BEDEBBBE;@@@B@@8:@B@@@@AE@@A0@>=<9:;9=7>:;==DBD=@DBBB=D6'=;;A=?=99=9.9.2,/2===79@@6@ +@L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT ++ +IIIIIIIIGIIIDGIIIFIIHIIIIIIIIIIIIIIEIFGFGGGHIIGIIIHBHIHGIGIHI@EG@EGFFIFEDFBC=;;2?-?=A? +@L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIBIIIGIIIIIIIIIIFIIIIIIIIIIIHGIIIIIIIEDGFGEDDGDBG:@@ECGEFEBHBHFHBB@@GGBBBDBECBBGECBECBFEBDEBEBB@@2B4?<=?;A??A@A+>? +@L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG ++ +IIIIIIIIIIIIHGGIIHIIIIIIIHIIIHIIIIIGIHHIIIIHDIIIHHHDIIIHIIHDHGEHEHGDGHEDGDHEEFEHG,EDCDCF@EBBECB@@B6>@A@ECBBCBBEEA>BBB@@DBAB=@8@@ +@L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHGHHHHGBHHHHHHHHHFHHHHEHHHHEFEHFGGFGAA>C=GGDBDEDEEBCBBEEBB>BD@BB@DBBBB=C>BCCB>EBB8? +@L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGBGGGEGGGG@GGDGHHFGHHHHHHGGFEGBGGG3CE>EAABGDBFFEDFE>E>DCB@EFFBC>EDBDD+CAA>>9?=99DCC?ACABEBBEBEECEBGDECDEEEEEED8B>@@@??EEB*B71;2>7@?===@ +@L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT ++ +IIIIIIIIHIIIHIIIIIIIIIIIIBIIIFIIIIIHIIHHHIIFFIIIFEIFIIIHIIIIIHGIEECHEFIDIBC>GBFFFEEHBEGEBBEFDBCBBF8A??ABBD=B@@DBB??A;;>? +@L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIHIIIIIIIIIHIIIIIGIFIIIIIIIIIHIIIHHIHHFIIIGIIIEGIFHIIGHIIIGHGIGIGHIEDBFAEHHFEGGDGECBC@BDB@ +@L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHGHHHHHHHHHGFGGGFGGEGGGGGGHHHHHGHHHHHHHHHEEEGCHHHGGGHHHBHGH>EEDBDEHHHDFBFG>B +@L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIGIIIIIIIIIIHIEIIIHIIIGIIIIIIHIHFIIIIIDIHHIGGIIIIHBHIFIFIIIHHIEE@GBGDDDD@EHEEEECBEEEDCFBCBBFEEI>@B@DBDBEEB9???,?@A=A=<=;*;? +@L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG ++ +IIIIIIIIIIIIGIIIIIIDIIIIIHIIIHIIIIIHIIHHIIIIHIIIHHFHGIHHIGGIEHDICDEIEIIEIBBBD@FBEEBEEEFDDBEFEBBBBEBBBBD?BB88<:<9?B@B@@@A?3@@@9?@ +@L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA ++ +HHHHHHHHDAGGGHHHHHHHHHHHHHHHHHHHHHGHHHHHHH@HHHHGHHFGHHHHHHHFHHHHGHHHDHHGHGGHGBC>DECEDB>6>>>A6>>;4>=6:=9=B:@ +@L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHHHHHFHHHEHEHHHHHHHBHHEHGHHHFGGGGBEEHEGCBGDCGEGGEGGEGECCEC@EE>B@A==@7,8=>;1=9A>>>;A +@L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIGIHHIIIIIGIIIIIFIIIHIIIIIHIIIHIIGIGIIIHIIFIIIGIIIDHGDIFIFEHIFBHBDFFFEIE8BDEDDEEFDDHECC@EABCEEBAD3D@BCCCEF>CBBBC8C?B@=@@<<>BB=@@B +@L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG ++ +GGGHHHHHGEGGGHHHHHHHHHHHHHHHHHGHHHHHBHHHHHHGCGBGEEGEEGGDBBADBGDFDEEGCGFEFFCDBBDBECCEFHEEEE:HB;A<::=?>=;EEEAE@>AC>,:.52?;:5255:,7@3:4:@ +@L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIGIIIIIIIIGIIIIIIGIGIIHIIIFIHIIIIHHIIIIIEIEIHIDDGG@GGBGGGHIIIIGIFIGIIHHEEEGBHEHHFFGHGGGGEGDEBEDEACDDCABBDDB@@@BEBEBCCBDBBBBDEE?B@>B +@L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHHHHHBHHHHHHHHHHHHHHHHHHHHHGHHHHHGHHHHHBGHHHHHGHHBGGEGEDGBGGHHH>HDBGDGHHGHED>GB@GEGEGHFBHED@BDDDHFD@C>FCEFEHBB>@?:>??8826=8/:;<<:?>)>A +@L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIIIIIIIIBHHIIHIHIIIIICIIIFIIIIIHIIIHGHHFEIHIEFIIBIHFHIIHIIGHEHGHGHHGHDBBICHEFHEGGDGEECGHEGDE>GGEDEGDDDBEEBEE@BBEBBDGDC=ABBBB?==-:==? +@L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG ++ +HIIIIIIIIIIIIIIIIIIIIIIIIIDIII3IIGGGGGIIIII@HIIGIIHGIIIIIHIIHIIIHIIDEGGEGGHHIHIHIIGDFIBFGFGGFGFEEGEFEF@EEEEFEBEDFDCD@BBEDE>EDCFBEDBEF>;A>:>AA8>>;AA +@L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ++ +IIIIIIIIIIIIFIHIIIIGIIIIIFIIIHIIIIIIIIIIDIHIIIHIGIIHIIIIIIIIFHHIGHGIHIGEDBBBGBGHFHGCGHGEBEEIEEBEECEEHCHBEFBBB@BBBC>E@B?@@?@=@@BB;>@?>@?>A +@L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG ++ +DGGEBEEE?B,B?GGGGGGGGGGGGGGGGG>GGDGGGGGGGGGGGGGGGGGGGGGA>>G@>GDDGDGFFGD8FBB??>GDGDBA@AD;AAAE5?B;;4ADDBBB@B@D1.?8??-8::;? +@L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIHHHIIIIIIIIHIBIIIFIIIIIHIIIIIIEFIIFIEHFHHIIFIIIIHIHIEIHIHIIFICECIBHHIFEHDEGECBBHEDCBDFCEEBHBBEC>B=BBBCEBDA@BB>B>BB@CB=?@ +@L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIHIIIDIIIIIIIIIIIIFIIIHIIIIIHIIIIHIIIIIIIIIIIIIEIIIIIIIGIHHEIHIHHICEHIHHII@EHEHFEGDGHGEFEEFGGBEHBEEBBBEBEB@@>@=@B +@L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA ++ +IIIIHIHIEGGGEIIIIIIIIIIIIGIIIHIIIEGBGDDDDGGGIIIBEHIIFHHIIFIIGDBC@>EC3@?>@GEGG@@DBD@E8CC=BBDBEAC,38==:;=@?,3=9-<;68<===B +@L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT ++ +HHHHHHHHEGGGGHHHHHHHHHHHHFHHHHHHHDBGGFHHHHBGGDDDHHHHGGHHBFGFCGGFBEGGDGD>D>9ACACEEEBEEBBCDB@EF>DBB@C3*=@AA@=@BAEEBCCB@C +@L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG ++ +IIIIIIIIFIIIIIIIIIIIHIIIHHIIIHIIHIIHIIIFHIIFHIIIHFHIIDHHIHIIIIHIEHDIGIHGFDDCFCB@DEBFEFCFC>EDDBBBBBAAA?A@BB@E@?>-@A@B??@DB-A +@L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIHIIGHIIIFHHIHIGIIIIHIIIIGHHIHIGHIECCGCFFIFFHDFGB=@@DBBBBBBDB?@EBBB=B>=B@BBBB +@L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG ++ +HIIIIIIIIIIIIDIIIHIIIIIIIBIIIGIIIIIGIGIHIFIHHIIIGHIIIFHHIGDFIEEIEFIDFHHGEGBHCFHGEGDGCDEBCCBBEEC>B@B>B8@BBB:B;???9@B>C@>;? +@L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC ++ +IIIIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIHIHHHIIHIHIEIIFEHHIIIIIIIHIGIHIBDHEHGHHHGHHEGDBCDF>DDCBFE@?BB@B@78BB;@=@@?@=;A=????@:>=@=B +@L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIGIIIHIIIIIHIIHHHIIIHIIIEEIFIIIFHIIIHHEIGGGIGIIGIGGDIEGGHGHDEEABCCEDBC@BBEBBDBFEEGABB@BCC<<<;:A0?A +@L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIGIIIIIIIIGGIIIIIIIIIIIIIIIIIIDIIIIIIIIHIHHHIIIHFIIHHIGIIIIHGEIDGFIIHIHFEIEHGIGHGGEG@EEBGEEBDEEEDGEHHDGHHGDDCGBDBB?DBD@B??A8A?@DBGGGGGGGDGGGGGGGG@GGGGGGGGGGGGGGGGGGGGGGGDGGDGCFF>?GGGGGGEGGGGGG8GGGGDGGBGCE>CECDEBEC?>,+?A=??==B@BBA<>>/=9?8?>>? +@L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG ++ +IIIIIIIIIIIIIFIIIIIGIIIIIBIIIFIIIGIFIIIHHHHIHIIIFHIGEIIHHIHHIH>IFIEIHHGHIBC>GCFEIEEHGEGDEEDGDBCBBBDDEEH@2@AEEB>BAA>>9>=AA77>>@? +@L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG ++ +BEEGGGGG3EBEDEEEEEGG3@>,EEGGADB??<16/459DFDGFFFDB15*9:/AAAAA(??;;FCCBDDD@>BBDBBB85>;A +@L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG ++ +BBBGGGGGDDGGGACA@,;<58;GDGGGGGGFGGBGGEGGGGGGGGEGEGEGBGEGGGEG8EEFEFEGFBFADCCEBDBFBEEEEAEBBBDD<>A8>>AEEBE@DBB8DB@B0>9)>>@=8@>:3:8@ +@L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +<@BGGGGGGGGGGGGGGGGGGGGGGDGGGGGEEGGGGGGGGGGGGGGGGGEGGGGGGBEGGGGGEGGGGGGGGBG2GBGDGGDEGDGFEGFGEGFFDEEGDEGEFDEGDDBDFC8FCBDDCCDDBFBC5AA>A>A8AAAA5@89? +@L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +IIIIIIIIGIIIHHIIIIIIIIIIIEIIIEIIIIIHIIHHHHHHHHIIGHEHIIIHIHFHICGHEFEIEIHEH@=BD@DEFECB:BBBCBCFCBBC=CCC@8E@CBBCBBBBBD8D@;AA@??BDDEGDBAAD=>@@@ +@L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG ++ +HHHHHHHHDGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHHFEHHHHEHHHHHHHHHHHDHHHHGGHGHGDHHHFGGGHHHHHHFHGHGHGCEE2EBA?CB@B@@BDBBB=B@=BBBBBBD7BBB +@L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG ++ +GGGGGGGGDGGGGHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHGHEHHHHHHHHEHFEFBGGHGGHHBEGEGFHBEHHBHGHHDHHHGHGBHC8>8?ECDEEEECB@/:::;5==>>A +@L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIIIIIHIIIIFGHHHIGIGHHIIIIIIHIIHHIIGFFIHHHGICHGHGHGGHEDHHGDFGGGGGGDB@BCCBDABE@E@BBBBEEBF@?A>?==B@/=?@?>;@BB@ +@L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIIIIIIIIIIIGHIIIIDIIIIIGIIIFIIIGIIIIIIIICGGIIFBDGG@IFFIFHGHIHDBECEHGHGFFD8FCGCGGGGFGEBDEEEBEEGEGBB>BB@BBCCA=;??=A@=?:>@??:@@(@ +@L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC ++ +IIIIIIIIIIIIIIIIIIIDIIIIIHGIIIIIIIIGIIIFIHIIHIIIGIHFIIHIIHIGHGGHEHEBEEEFCGEGDBEECEBBCC<@BDBBEDBDBD@BCBCDDDADB@==@?@B=@@B3B=@@ +@L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG ++ +HHHHHHHHHHDHFHHHHHHHHHHHHGHHHGHHHHHHHHHHHHHHHHHHGGGG>HHHHHEDGDDGFGGGDGGBGEEGBGG8G@DEGGEFEFEBBBDBBD@>?:;??=?B +@L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG ++ +HHHGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHHGG1D>D;A9A8C +@L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +HHHH@HHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHHHHHHHHHGEHHHFHEGEDGDDDGGFFFDDABEEEDDCFGEEDBCAEBEEEEE@EBBEBBEEEE8B@@:<8==<=BAA>A@:@8@ +@L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIIIIIIIHIIIIIIIIIIHIIIIIIIIIIIHIHFIIHIIHIHHHIIIHHIHHIIIHIGIHIFHIFHEDBIDFFEHHGGHHEFEBIEFFCEED>EDEBEEDBEEHDBEFEBDGEEDDBFEDEBCBBB;CBBB +@L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG ++ +IIIIIIIIIIIIIHIIIIIIIIIIIHIIIFIIIHIHIIHHIHHIIIIIHFIIGIFDAGIIHFHHEH@IHIIGIHGGIEHGHEHGHFHGEEEIHHEEE@EEEEIEFHBEDFGDFGGEEDEEBB18A@@@B@88@ +@L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIHIGIIGIIIIGIIIGIIIHIIIIIHHHIIIHIIIHIHHIIIHHGIGE>FEEEFHEEGGGGCHEHGGEGDEEGG@GDDEDGEBEGDBEEEDEBEBBDDCCDDC>DCC@C?CBCE@BBBB>9@<>?;@+;CCEB>B;/=?=BCBCB>BB>@@B<@?<85*928578=@B +@L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHGHHHHHHHGHHHHHHFHHHHHFHHHHHHHHHHHHHHHHHHEHHHEEGDGHHHHHHHHHHHGHHHHHHHHDGGHGHHHHFEHGGBHBEHEGHGHGGHGDHAEHEHBBDBBBBC>BEEEBBAEAAEEBEAB><@B +@L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +HHHHHHHHEHHHHHHHHHHGHHHHHHHHHDHHHHHHHHHHHHHHHHHHHHDHHHHEFHHHHH@HHHHHEHHEGEEEHDHEFHEGHE@EHGGHGC@B8DEEECEBEED8;?=??DBCDBCCCBDBBCECGGHH@GGCFGGEGHGB@BBBEEEEH@BBCCB@B>CE=A@@=:==? +@L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG ++ +EEEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGFGFGGGGDGFGGF>EGEGEFED8DGDEEEBGDGBDFDBDBDEE8>+>;;49;? +@L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT ++ +IIIIIHIDIIIIIDIIIIIIIIIIGDIIIEIIIIIIIIFFFIIIHIIIHEIIIIGFIIIGIIHIEHEIFDIFIGGGHEIEDHCGHGHHDEEIEEEEEEGGG>GDEDBB>ADAD@A;>4<=<967:<<=:? +@L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC ++ +HHHHHHHHHHHHHHDHHHHHHHHHHHGHHHHHHHHEHHGGBGGGHHHHHHHHHHGHHHHHHGEGFFGFFGFDFEFHFDHEEHBC2BEC8=??ABBB@D@@>BBBB@5@77==1CC@BB +@L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG ++ +GGGIIIIIIIIIIIHIIIIGIIIIIDIIIGIIIIIIIHIIIGIIGIIHEGGGCIIFIIIHIIIIHGHIHHIEHHEDDHFBIDFFHHFEGGGHG>EEBEBCEBC@BBBB=69=,:186:EBEBC;;@:@ +@L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIGIIIIHIIIIIIHHIIIIIIIIIHIIHHIIIIHIIIHGHHIIIHHIHIIIHIEEEIHIIGIFCDIBHHI@HHGEDD>B@DBEBBBEEEBEFCEGEEBE>>)>@EDCC@B<>:5:6:>68>0A;9>;:@?<>??@B +@L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG ++ +GGGHHHHHHHGHHHHHHHHGHHHHHGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGBHHHHHBHHHHHHHFHGHBHHFGEGEEBEHHHDDDB@GB=?=?EEE>EBD3=@AABDDD>BBCAAECDCDB@BB@:C@BB@B=0=0>?=>@ +@L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ++ +IIIIIIIHIIIIHIIIIIIIIIIIIIIIIIIIIHIIIIIIHIFIIIIIHHIFIIIHGHHIHIIIHHIIFFICIB8BDGD@DBACFEDGCEEBFFFCBBBEBEBAA;8?>?AAABBCC@BB>>C@DB@B@7@@@83;8@89<7+=8=(=5?9? +@L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG ++ +<@@GGGGGGGBGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGBGGGGGGDGGGB@>ADAEEE@D?A=<=@B@@@B@B>BBBBCA@@)>@<4>1>@@>;>A4:@A +@L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIIDIIIIIIIIIHIIHIIIIIHIIIHDIFIIIIIIIIIIHIGIGIHHIEIFFEIEIHIFIGGHGHDHHHGHGBB@@BBB=BBDB?@B:BBBEEECB??A4@4?==? +@L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG ++ +=B@GDGGGEGEGGGGGGGGEGGGGGDGGGGEEEGGGBGGGGGGGDGGGGGGG@EEBBE???DBGGGEGBGGDGGGGBC?0?BDGDDGCBDDDDDDDDEGGFDDDAG+(2:39DBAAA +@L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG ++ +IIIIIIIIHIIIIIIIIIIGIIIIFFIIIFIIIIIHIIIHEIIIHIIIHHIFIIIIHIIIHHHIGHHFHIHEIDBBFGHFHFEHEIIEBDBEEDCDEF=DBDDCBD=D@BDEBDFCC>B@B4B=@=>@=BB>8? diff -r 000000000000 -r c1bd0c560018 test-data/split_fastq_libraries_histograms.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_histograms.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,8 @@ +Length Count +116.0 20 +126.0 31 +136.0 33 +146.0 2 +156.0 0 +-- + diff -r 000000000000 -r c1bd0c560018 test-data/split_fastq_libraries_log.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_log.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,53 @@ +Input file paths +Mapping filepath: /home/cidam/Berenice/asaim/framework/lib/galaxy-master/database/files/000/dataset_15.dat (md5: 4f17f8d2fbfd80c72d7958f39c5c5286) +Sequence read filepath: /home/cidam/Berenice/asaim/framework/lib/galaxy-master/database/files/000/dataset_12.dat (md5: 9d4f2526cd83c61358d737e8ead741bd) +Barcode read filepath: /home/cidam/Berenice/asaim/framework/lib/galaxy-master/database/files/000/dataset_11.dat (md5: 3856d8f889198dc37ffd61b231c03257) + +Quality filter results +Total number of input sequences: 250 +Barcode not in mapping file: 134 +Read too short after quality truncation: 21 +Count of N characters exceeds limit: 6 +Illumina quality digit = 0: 0 +Barcode errors exceed max: 3 + +Result summary (after quality filtering) +Median sequence length: 132.50 +L1S76 14 +L1S281 12 +L1S8 11 +L1S208 11 +L1S140 11 +L1S105 10 +L1S257 9 +L1S57 8 +L6S93 0 +L6S68 0 +L6S20 0 +L5S240 0 +L5S222 0 +L5S203 0 +L5S174 0 +L5S155 0 +L5S104 0 +L4S63 0 +L4S137 0 +L4S112 0 +L3S378 0 +L3S360 0 +L3S341 0 +L3S313 0 +L3S294 0 +L3S242 0 +L2S382 0 +L2S357 0 +L2S309 0 +L2S240 0 +L2S222 0 +L2S204 0 +L2S175 0 +L2S155 0 + +Total number seqs written 86 +--- + diff -r 000000000000 -r c1bd0c560018 test-data/split_fastq_libraries_sequence_qualities.qual --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_sequence_qualities.qual Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,584 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[32 34 34 34 38 38 38 38 31 38 30 38 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 35 38 38 33 38 38 38 33 38 38 38 38 38 38 + 38 35 38 36 38 38 35 35 38 31 38 38 38 38 36 36 36 38 38 38 38 38 38 18 23 + 23 38 35 29 35 35 38 38 36 36 31 35 35 35 35 33 29 37 35 38 35 29 31 12 25 + 22 19 28 26 32 9 22 19 28 28 28 28 30 33 33 35 31] +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 37 + 39 33 39 39 36 34 34 39 33 35 39 37 36 39 32 36 31 31 33 35 33 28 33 31 31 + 32 17 35 7 20 20 20 18 35 32 32 32 32 36 31 36 36 32 29 26 8 24 26 28 28 + 32 29 22 33 31 28 31 22 23 26 25 23 17 30 34 27 29 27 24 30 27 29 29 29 24 + 23 28 31] +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 38 40 40 40 40 38 40 40 40 40 40 38 40 40 39 40 35 40 39 40 39 39 + 37 31 40 40 37 40 40 40 40 40 38 40 40 40 39 40 39 38 39 33 40 40 40 38 39 + 39 38 40 37 36 36 36 37 39 39 39 35 36 36 35 38 29 35 33 36 37 33 35 33 31 + 33 33 28 36 36 33 33 33 36 38 36 35 35 33 32 29 35 31 31 31 31 33 34 33 33 + 26 33 35 31 31 29 25 31 31 29 33 31 30 28 16 31 31 31 18 27 24 22 21 22 28 + 21 29 26 26 30] +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 38 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 38 36 38 38 35 40 40 40 40 40 37 37 39 40 40 38 35 38 36 38 35 29 + 38 35 38 40 40 36 40 38 40 40 37 39 29 38 33 35 35 38 37 38 38 36 38 33 33 + 17 35 33 35 35 38 33 35 36 33 36 33 33 23 30 27 33 29 33 29 32 31 35 28 18 + 23 28 32 34 29 30 29 31 31 30 35 35 31 29 28 30 28 19 22 23 26 10 26 33 36 + 23 29 33] +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 39 36 36 40 40 36 40 40 40 40 40 40 38 + 38 39 40 39 36 40 40 39 35 37 34 40 38 39 39 39 34 36 35 33 40 36 40 36 36 + 39 33 33 36 33 33 36 40 39 38 38 38 38 38 35 39 38 38 36 36 38 35 38 30 27 + 17 32 20 35 33 33 33 33 36 33 36 33 33 33 31 33 36 35 36 33 33 33 36 26 31 + 31 31 33 31 31 23 25 31 33 31 31 31 31 32 36 31 31 32 15 31 29 28 27 24 25 + 26 24 28 22 29 25 26 28 28 27 30] +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 38 38 36 38 36 38 38 38 38 38 39 39 39 39 39 39 39 38 39 39 38 38 + 34 38 38 39 37 39 39 39 39 39 39 39 39 36 35 33 38 38 39 37 39 39 39 39 37 + 39 39 39 39 39 37 39 39 39 39 39 36 33 37 34 36 36 35 37 37 37 35 36 28 26 + 31 34 28 34 34 34 32 32 33 33 35 31 31 25 28 26 30 28 33 33 31 29 35 33 35 + 28 31 35 33 33 33 28 35 21 6 28 26 26 32 28 30 28 24 24 28 24 13 24 13 17 + 11 14 17 28 28 28 22 24 31 31 21 31] +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 35 38 40 40 40 37 40 40 39 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 36 40 37 38 37 38 38 38 39 40 40 38 40 40 40 + 39 33 39 40 39 38 40 38 40 39 40 31 36 38 31 36 38 37 37 40 37 36 35 37 33 + 34 27 37 36 34 31 33 10 31 33 33 33 33 23 17 28 28 30 33 33 31 27 33 33 31 + 33 33 8 32 32 31 23 28 31 30 31 31 20 30 27 29 28 26 26 17 30 12 30 28 32 + 30] +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 + 40 40 39 38 40 40 40 40 40 40 40 36 35 38 37 38 36 35 35 38 35 33 38 25 31 + 31 36 34 38 36 37 36 33 39 33 39 37 39 33 33 31 31 38 38 33 33 33 35 33 36 + 34 33 33 38 36 34 33 36 34 33 37 36 33 35 36 33 36 33 33 31 31 17 33 19 30 + 27 28 30 26 32 30 30 32 31 32 10 29 30] +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 38 38 40 40 39 40 40 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 38 40 39 39 40 40 40 40 39 35 40 40 40 39 39 + 39 35 40 40 40 39 40 40 39 35 39 38 36 39 36 39 38 35 38 39 36 35 38 35 39 + 36 36 37 36 39 38 11 36 35 34 35 34 37 31 36 33 33 36 34 33 31 31 33 21 29 + 31 32 31 36 34 33 33 34 33 33 36 36 32 29 33 33 33 31 31 35 33 32 33 28 31 + 23 31 31] +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 38 39 39 39 39 38 33 39 39 39 39 39 + 39 39 39 39 37 39 39 39 39 36 39 39 39 39 36 37 36 39 37 38 38 37 38 32 32 + 29 34 28 38 38 35 33 35 36 35 36 36 33 34 33 33 36 36 33 33 29 33 35 31 33 + 33 31 35 33 33 33 33 28 34 29 33 34 34 33 29 36 33 33 23 30] +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 33 38 38 38 36 38 38 38 38 31 38 38 35 38 39 39 + 37 38 39 39 39 39 39 39 38 38 37 36 38 33 38 38 38 18 34 36 29 36 32 32 33 + 38 35 33 37 37 36 35 37 36 29 36 29 35 34 33 31 36 37 37 33 34 29 36 35 33 + 35 35 10 34 32 32 29 29 24 30 28 24 24 35 34 34 30 32 34 32 33 36 33 33 36 + 33 27 33 33 35 31 33 31 31 31 33] +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 + 40 40 40 40 40 40 40 40 40 40 39 40 40 38 40 40 38 40 40 40 40 39 40 40 40 + 40 35 38 40 33 37 39 35 39 33 36 39 39 40 36 35 39 38 39 39 40 37 37 38 38 + 39 31 29 36 36 34 36 33 38 35 36 34 35 36 36 36 36 36 36 35 23 33 29 31 31 + 31 30 30 36 36 33 9 33 22 16 26 17 29 22 31 30 28 28 28 31] +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 39 39 39 40 40 37 37 40 40 40 37 36 + 40 37 40 40 40 39 40 40 40 40 40 39 38 40 36 36 34 39 36 37 40 35 40 33 34 + 29 38 33 37 37 37 36 36 39 33 36 38 36 33 33 36 37 35 33 34 33 33 37 23 32 + 30 30 32 33 33 35 28 33 31 31 35 33 33 30 30 32 26 26 29 30] +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 38 40 + 37 40 40 40 40 40 40 40 40 40 39 40 40 40 39 39 40 39 39 37 40 40 40 38 40 + 40 40 36 38 40 37 39 40 40 38 39 40 40 40 38 39 38 40 38 40 38 39 40 36 35 + 33 37 32 36 39 39 37 36 38 38 35 38 36 27 34 33 37 34 38 35 36 38 38 38 38 + 36 38 39 36 36 34 36 29 34 33 34 31 33 35 33 31] +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 37 38 38 38 37 38 + 38 36 38 38 38 38 38 38 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 36 36 + 36 38 34 39 39 39 38 38 38 39 39 39 33 39 38 39 29 36 36 35 33 35 36 39 39 + 39 35 37 33 37 38 29 27 30 30 34 32 34 23 35 35 32 33 35 36 36 33 36 35 33 + 31 33 36 33 31 33 29 33] +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 40 + 36 40 40 40 39 40 40 40 38 40 40 40 40 40 40 39 40 39 37 40 40 40 40 40 35 + 40 39 39 40 38 38 40 40 40 40 39 33 39 40 37 40 37 40 40 40 39 39 40 36 36 + 31 38 33 38 35 35 35 35 31 36 39 36 36 36 36 34 33 36 36 36 35 34 37 33 34 + 33 33 37 36 36 40 29 31 33 31 35 33 35 33 36 36 33 24 30 30 30 11 30 27 32 + 29 31 32 28 32 28 27 28 26 9 26 30] +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 35 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 39 + 37 39 38 40 39 39 40 38 38 40 36 39 35 40 34 35 36 40 36 40 40 36 40 33 33 + 33 35 31 37 33 36 36 33 36 36 36 37 35 35 33 36 37 36 33 33 33 33 36 33 33 + 33 33 35 30 33 33 23 23 27 25 27 24 30 33 31 33 31 31 31 32 30 18 31 31 31 + 24 30 31] +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 32 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 31 39 39 39 39 38 39 39 + 37 38 39 39 39 39 39 39 39 37 39 39 39 39 38 39 39 39 35 39 39 38 39 38 38 + 39 27 38 36 31 35 36 33 36 37 36 37 39 38 38 38 33 38 31 33 33 36 35 33 35 + 33 31 35 36 33 37 36 36 38 36 36 29 38 33 34 29 35 36 34 36 35 33 29 21 29 + 29 29 32 21 29 29 26 19 29 28 21 25 28 24 28 33 25 31] +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 36 39 39 39 39 39 39 39 37 39 39 39 36 39 36 39 39 39 39 39 39 39 33 + 39 39 36 39 38 39 39 39 37 38 38 38 38 33 36 36 39 36 38 34 33 38 35 34 38 + 36 38 38 36 38 38 36 38 36 34 34 36 34 31 36 36 29 33 31 32 28 28 31 22 11 + 23 28 29 26 16 28 24 32 29 29 29 26 32] +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 38 40 39 39 40 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 39 40 40 38 40 38 40 40 40 39 40 + 40 37 40 40 40 38 40 40 40 35 39 38 35 40 37 40 37 36 39 40 37 33 39 33 35 + 37 37 37 36 40 36 23 33 35 36 35 35 36 36 37 35 35 39 36 34 34 31 36 32 33 + 34 36 36 33 32 35 18 35 31 33 34 34 34 36 37 29 34 33 33 33 34 23 34 30 33 + 31 28 31 31 27 27 29 33 33 28 31 31 33] +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[38 38 38 39 39 39 39 39 38 36 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 33 39 39 39 39 39 39 38 34 38 33 38 36 36 + 38 36 36 38 38 35 33 33 32 35 33 38 35 37 35 36 36 38 34 38 37 36 37 37 34 + 35 33 33 35 33 36 34 34 36 37 39 36 36 36 36 25 39 33 26 32 27 25 25 28 30 + 29 28 26 36 36 36 32 36 31 29 32 34 29 11 25 13 20 17 30 26 25 20 17 20 20 + 25 11 22 31 18 25 19 25 31] +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 38 40 38 40 40 39 + 40 40 40 37 40 39 40 40 40 40 39 39 40 40 40 40 40 36 40 36 40 39 40 35 35 + 38 38 31 38 38 33 38 38 38 39 40 40 40 40 38 40 37 40 38 40 40 39 39 36 36 + 36 38 33 39 36 39 39 37 37 38 39 38 38 38 38 36 38 35 36 33 36 35 36 32 34 + 35 35 34 32 33 33 35 35 33 31 31 31 33 36 33 36 33 34 34 33 35 33 33 33 33 + 35 36 36 30 33 31 29 33] +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[38 38 38 39 39 39 39 39 33 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 38 39 39 39 39 39 33 38 39 39 39 39 39 38 + 39 39 33 38 38 36 38 36 35 38 33 38 38 39 39 39 29 39 35 33 38 35 38 39 39 + 38 39 36 35 29 38 33 31 38 36 38 36 38 39 37 33 39 36 35 31 33 35 35 35 39 + 37 35 31 34 29 37 34 36 37 36 39 33 33 29 31 30 25 29 30 30 23 23 17 21 28 + 23 14 25 26 27 27 25 30 29 8 29 32] +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 33 39 39 40 40 39 40 39 40 40 40 40 40 + 34 40 40 40 37 40 40 40 40 40 39 40 40 40 39 38 39 39 37 36 40 39 40 36 37 + 40 40 33 40 39 37 39 40 40 39 40 40 38 39 36 39 38 39 38 39 39 38 39 35 33 + 33 40 34 39 36 37 39 36 38 38 35 38 36 36 34 38 39 36 38 35 36 29 38 38 36 + 35 36 38 35 35 35 33 36 36 33 36 36 31 33 33 36 33 33 35 38 35 34 28 32 33 + 33 33 33 30 28 28 12 25 28 28 30] +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 40 35 40 40 40 18 40 40 38 38 38 38 38 40 40 40 40 40 31 39 40 40 38 40 40 + 39 38 40 40 40 40 40 39 40 40 39 40 40 40 39 40 40 35 36 38 38 36 38 38 39 + 39 40 39 40 39 40 40 38 35 37 40 33 37 27 35 33 35 35 33 40 35 39 39 40 40 + 38 38 38 37 40 37 36 39 32 32 34 36 32 36 40 35 40 24 40 40 37 34 37 37 36 + 36 36 37 27 33 33 27 35 28 28 30 30 30 27 31] +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 38 40 39 39 39 39 39 39 40 39 40 39 40 35 36 + 40 38 34 40 37 39 39 40 39 36 40 39 36 39 36 39 36 40 36 40 39 36 40 33 35 + 33 37 33 37 34 35 35 35 38 36 38 36 36 35 35 35 38 34 33 35 33 33 35 32 33 + 36 33 36 35 38 35 36 32 36 33 34 33 34 33 33 36 33 33 31 36 33 22 33 33 31 + 33 33 33 21 30] +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[34 34 34 38 33 38 38 38 36 38 38 38 38 38 38 38 36 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 35 38 38 37 38 38 38 38 38 38 38 38 37 35 35 38 37 38 + 38 36 38 38 38 33 36 38 36 38 35 38 38 38 37 38 29 38 37 38 38 37 38 37 36 + 36 38 36 37 36 37 31 36 36 36 36 37 36 33 36 35 37 35 34 35 31 33 33 36 35 + 36 29 36 35 34 37 33 36 35 33 36 37 29 26 32 29 25 29 32 32 23 29 29 26 32 + 32] +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 37 40 39 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 40 40 40 40 40 35 40 39 40 40 40 39 40 38 40 + 40 39 40 40 40 40 40 40 40 40 37 39 39 40 38 39 38 40 39 40 38 36 35 33 33 + 33 38 33 38 39 37 39 38 34 38 39 38 36 33 36 36 40 36 36 33 36 36 34 36 36 + 39 34 39 33 36 37 33 33 33 31 33 33 33 34 29 36 31 33 30 31 31 30 31 28 31 + 31 33 33 26 29 31 30 29 31 30 29 32] +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[35 38 38 36 33 36 36 36 30 33 11 33 30 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 29 38 38 35 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 32 29 29 38 31 29 38 27 36 30 33 36 36 31 38 38 35 38 38 35 + 38 33 35 38 36 33 29 35 35 38 35 38 37 37 38 35 23 37 33 33 30 30 29 38 35 + 38 35 33 32 31 32 35 26 32 32 32 36 20 30 33 26 26 19 32 35 35 33 33 33 31 + 33 31 35 16 13 30 23 30 30 12 23 25 25 26 30] +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 39 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 40 40 40 40 36 37 40 40 37 40 36 39 + 37 39 39 40 40 37 40 40 40 40 39 40 39 40 36 40 39 40 39 40 40 37 40 34 36 + 34 40 33 39 39 40 37 36 39 35 36 38 36 34 33 33 39 36 35 34 33 35 37 34 36 + 36 33 39 33 33 36 34 29 33 28 33 33 33 34 36 33 35 32 31 33 33 29 33 29 33 + 33 31 34 33 28 30 31] +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 35 40 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 + 40 40 40 40 36 40 40 40 40 40 40 40 38 40 39 39 36 40 39 40 39 39 40 34 36 + 39 40 39 39 40 40 31 36 39 36 39 37 36 38 35 38 39 38 36 37 36 36 37 38 38 + 33 36 39 33 36 36 27 37 31 31 33 35 35 35 34 33 35 33 35 35 33 33 33 29 33 + 33 33 36 33 36 33 31 31 29 31 28 31 33] +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 39 40 36 38 38 38 36 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 36 38 33 38 35 35 35 35 38 38 38 40 40 40 33 36 39 + 40 40 37 39 39 40 40 37 40 40 38 35 33 34 31 29 36 34 18 31 30 29 31 38 36 + 38 38 31 31 35 33 35 31 36 23 34 34 28 33 33 35 33 36 32 34 11 18 23 28 28 + 25 26 28 31 30 11 18 28 24 12 27 26 27 30 32 30 30] +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 36 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 38 36 33 36 36 38 36 38 38 37 38 34 34 + 33 38 31 36 36 37 35 36 36 36 38 35 36 36 33 33 39 36 35 36 33 36 36 38 38 + 33 36 38 33 32 36 33 35 31 32 39 35 34 30 34 36 31 30 33 35 33 25 29 21 23 + 27 28 28 28 33] +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 37 39 39 39 39 39 39 39 35 33 38 38 37 39 39 39 39 33 38 38 35 35 35 39 39 + 39 39 38 38 39 39 33 37 38 37 34 38 38 37 33 36 38 38 35 38 35 29 35 29 24 + 32 34 32 34 36 36 36 33 36 36 33 33 34 35 33 31 36 37 29 35 33 33 31 34 18 + 9 28 31 32 32 31 28 31 33 32 36 36 33 34 34 33 31 34] +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 39 + 39 40 40 40 39 40 40 39 40 40 39 40 40 40 37 39 40 40 37 39 40 40 40 39 37 + 39 40 40 35 39 39 40 39 40 40 40 40 39 40 36 39 35 40 38 40 39 38 37 35 35 + 34 37 34 33 31 35 36 33 37 36 37 34 37 34 29 36 35 35 33 33 33 33 33 32 32 + 32 30 32 31 33 33 31 36 31 30 29 12 31 32 31 33 30 30 31 35 33 12 32] +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 38 39 40 40 40 37 39 + 39 40 39 40 38 40 40 40 40 39 40 40 40 40 38 39 39 40 39 40 38 39 40 36 34 + 34 38 34 37 37 40 37 37 39 35 37 38 33 28 31 31 35 33 33 33 33 33 33 35 33 + 30 31 36 33 33 33 28 33 29 28 33 31 33 33 33 33] +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 39 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 38 40 38 40 39 40 37 40 39 39 40 40 40 38 39 + 40 40 40 37 39 39 40 38 35 37 40 36 36 40 36 37 40 35 37 39 39 38 36 38 33 + 39 34 37 39 38 36 38 35 38 34 35 36 33 34 34 33 33 36 36 34 29 33 31 33 29 + 33 23 31 33 33 33 25 33 26 30 30 30 24 31 33 29 34 31 29 26 30] +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 39 40 39 40 36 40 + 40 37 36 39 39 40 40 40 40 40 40 40 39 40 38 40 39 40 33 35 39 36 39 38 39 + 39 39 38 39 39 36 38 35 33 34 35 37 29 35 35 34 33 37 36 31 30 33 33 31 33 + 31 22 23 33 33 26 31 28 31 31 30 31 28 26 32 28 30 30 30 30 31 25 29 28 31 + 28 33] +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 39 40 40 39 39 39 40 40 40 39 40 40 40 36 36 + 40 37 40 40 40 37 39 40 40 40 39 39 36 40 38 38 38 40 38 40 40 38 40 38 38 + 35 40 36 38 38 39 38 39 35 36 36 32 33 34 34 36 35 33 34 31 33 33 36 33 33 + 35 33 37 36 36 38 32 33 33 31 33 34 34 27 27 30 6 10 28 29 27 27 26 25 32 + 15 30 32] +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 38 40 40 40 40 40 40 40 40 38 38 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 35 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 40 + 39 37 40 40 39 39 40 38 40 40 40 40 39 38 36 40 35 38 37 40 40 39 40 39 37 + 36 40 36 39 38 40 38 39 38 38 36 38 31 36 36 33 38 36 36 33 35 36 36 36 35 + 38 36 39 39 35 38 39 39 38 35 35 34 38 33 35 33 33 30 35 33 35 31 33 30 30 + 32 23 32 30 31 35 27 33 22 12 30] +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[32 29 33 38 38 38 38 38 38 38 35 38 38 38 38 38 38 38 38 31 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 35 38 38 35 38 34 37 + 37 29 30 38 38 38 38 38 38 36 38 38 38 38 38 38 23 38 38 38 38 35 38 38 33 + 38 27 38 36 38 27 38 38 36 35 36 38 38 38 38 36 36 38 16 28 30 30 33 36 37 + 35 29 34 36 29 34 36 34 35 36 33 36 34 30 29 11 10 30 32 28 30 30 28 28 33 + 31 33 33 32 27 29 29 14 28 24 30 23 30 29 29 30] +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 38 40 40 40 40 40 + 33 40 40 40 37 40 40 40 38 40 37 40 40 40 39 39 39 39 40 39 40 40 40 37 39 + 40 38 36 40 40 39 39 40 39 39 40 39 29 40 37 40 36 40 39 39 38 39 40 33 34 + 29 38 34 37 36 40 36 36 39 38 36 38 35 36 36 35 38 35 33 34 33 33 33 35 35 + 36 36 39 31 27 33 33 33 36 33 34 33 36 36 37 36 36 33 36 36 36 31 32 25 30 + 31 33 33 27 20 23 6 17 27 27 27 21 24 26 30 32] +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[36 37 37 38 38 38 38 38 38 33 35 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 33 38 + 36 38 38 36 38 31 38 38 38 38 36 37 37 38 36 34 38 38 34 34 36 32 34 35 35 + 35 23 38 26 31 32 31 31 31 33 35 35 33 37 36 39 36 35 33 29 17 31 32 36 36 + 33 29 33 32 32 29 29 24 29 28 32 32 22 22 29 29 31 30] +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[33 36 36 38 38 38 38 38 18 36 33 36 35 36 36 36 36 36 38 38 18 27 38 36 36 + 35 27 36 38 38 31 36 35 38 38 33 27 38 36 36 36 36 32 33 13 19 33 33 20 29 + 31 29 11 36 36 27 36 36 38 38 38 38 35 38 37 38 38 38 29 38 38 32 35 33 30 + 30 27 16 21 14 19 20 24 35 37 35 38 37 37 37 35 33 16 20 9 24 25 14 32 32 + 32 32 32 7 30 30 26 26 37 34 34 33 35 35 35 31 29 33 33 35 33 33 33 23 20 + 29 26 32] +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[33 33 33 38 38 38 38 38 35 35 38 38 38 32 34 32 31 11 26 27 20 23 26 38 35 + 38 38 38 38 38 38 37 38 38 33 38 38 36 38 38 38 38 38 38 38 38 36 38 36 38 + 36 38 33 38 36 38 38 38 36 38 23 36 36 37 36 37 36 38 37 33 37 32 35 34 34 + 36 33 35 33 37 33 36 36 36 36 32 36 33 33 33 35 35 27 29 32 23 29 29 32 36 + 36 33 36 31 35 33 33 23 35 33 31 33 15 29 24 8 29 29 31 28 23 31 29 25 18 + 25 23 31] +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[27 31 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 36 38 38 38 38 38 38 33 36 38 38 38 38 38 36 38 38 38 38 38 38 38 38 33 38 + 17 38 33 38 35 38 38 35 36 38 35 38 37 36 38 37 38 36 38 37 37 35 36 36 38 + 35 36 38 36 37 35 36 38 35 35 33 35 37 34 23 37 34 33 35 35 34 34 35 35 33 + 37 33 34 20 32 32 29 32 29 32 27 32 32] +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 + 40 39 40 40 40 40 31 40 40 40 35 40 40 40 40 40 40 40 36 39 40 39 40 39 40 + 40 39 40 40 40 40 40 37 39 40 40 39 38 40 38 39 36 40 40 40 37 39 40 38 39 + 38 35 39 39 39 34 38 36 34 34 35 36 38 39 35 36 35 38 36 33 33 33 36 35 38 + 35 33 38 34 34 34 30 32 31 28 33 31 31 23 33 31 33] +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 36 39 34 35 + 31 38 35 39 33 39 39 39 39 36 38 38 36 36 32 34 36 31 36 34 36 34 34 33 35 + 33 27 35 28 35 31 31 32 29 23 32 32 32 32 20 31 23 24 30] +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 39 39 40 40 40 40 40 40 40 40 40 40 40 + 36 40 40 40 36 40 40 40 40 40 39 40 40 39 39 39 39 39 39 39 39 40 40 38 39 + 36 39 40 40 40 39 40 39 37 39 40 34 38 39 36 37 36 40 36 40 39 36 39 31 28 + 33 35 31 35 36 37 36 34 33 25 33 33 33 34 33 34 37 34 33 33 34 28 34 34 34 + 31 23 36 31 34 33 33 34 33 33 33 33 33 35 23 35 31 26 32 32 31 30 30 33 35 + 35 36 38 35 33 32 32 35 28 29 31 31 31] +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 37 36 + 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 38 38 39 38 + 39 38 35 39 39 39 37 38 38 38 39 39 39 39 39 39 37 39 38 39 38 39 38 34 36 + 36 17 36 33 27 32 35 31 36 36 36 33 33 36 38 32 38 36 34 36 33 33 23 16 30 + 26 30 30] +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 39 40 38 + 39 40 40 40 39 40 40 40 40 40 39 40 40 38 39 40 40 40 40 39 40 40 40 39 40 + 40 38 40 40 40 39 40 39 40 40 40 39 39 40 38 40 39 40 39 40 40 38 40 35 34 + 33 40 36 37 39 39 37 39 35 38 39 38 36 35 33 36 38 34 36 35 34 35 36 34 33 + 33 33 36 31 35 35 31 33 32 29 32 30 34 33 31 33 31 31 33 35 33 33 33 28 33 + 31 28 33 33 33 33 33 33 35 22 33 33 33] +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 38 38 38 38 38 35 38 38 38 38 39 39 39 39 39 39 37 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 36 39 39 39 + 39 39 39 39 39 36 39 37 36 37 33 38 38 39 38 38 39 39 33 36 38 36 38 37 39 + 33 36 39 39 33 39 38 39 39 35 39 39 39 38 39 38 33 39 34 23 29 23 30 36 34 + 35 36 36 36 36 34 27 33 34 36 36 33 31 24 16 26 28 28 33 33 25 33 31 33 33 + 29 33 31 14 25 25 25 26 20 28 28 29 29 32] +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 37 38 39 39 39 40 38 40 38 39 + 39 40 40 40 40 40 40 39 40 40 39 39 40 40 38 37 37 40 39 39 39 38 40 34 39 + 38 39 38 39 38 38 39 36 35 39 39 38 35 37 38 38 38 38 38 38 35 33 31 33 34 + 34 33 35 32 33 36 31 36 31 33 33 33 33 36 36 33 37 31 30 32 29 30 28 28 33 + 31 14 28 30 31 30 29 26 31 33 33 31] +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 39 40 40 40 40 35 40 40 40 40 40 + 38 40 40 40 37 40 40 40 38 40 40 40 40 40 40 40 40 34 38 38 40 40 37 33 35 + 38 38 31 40 37 37 40 37 39 38 39 40 39 35 33 36 34 36 39 38 39 38 37 37 35 + 23 37 34 38 34 38 38 38 38 37 38 36 33 35 36 36 36 33 36 36 38 36 38 33 33 + 29 33 33 31 33 33 34 34 32 28 26 30 30 28 27 30 26 19 29 32 31 28 30 25 29 + 31 30 30 25 31 31 7 31] +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 40 40 + 39 38 40 40 40 40 40 40 40 40 38 40 40 40 37 40 39 40 40 39 40 40 40 38 40 + 39 37 40 40 39 40 40 39 40 38 39 38 38 39 36 39 27 39 38 39 38 36 37 33 34 + 29 36 33 36 36 36 37 34 38 36 38 35 33 36 36 34 36 33 33 34 34 27 31 33 35 + 33 33 36 35 33 35 33 35 31 33 34 33 34 35 35 35 32 35 33 31 28 28 31 30 31 + 33 28 31 31 33 18 33 28 31 31] +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 35 39 37 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 38 + 38 38 29 39 39 39 39 39 36 35 38 35 35 38 37 38 38 38 35 38 38 33 38 36 36 + 38 33 38 38 23 38 31 35 36 38 38 36 37 36 37 36 27 38 38 36 36 37 36 38 38 + 39 38 18 35 38 33 33 33 35 35 38 35 31 38 37 35 36 36 35 33 35 33 33 29 33 + 33 33 35 33 33 35 31 29 30 25 26 30 30 28 30 33] +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 38 38 38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 + 39 39 39 38 38 16 35 27 30 32 26 28 26 38 37 38 33 38 38 27 38 35 38 35 35 + 38 38 33 32 32 35 38 31 39 39 33 39 37 38 39 36 38 36 27 30 28 23 23 35 35 + 35 32 35 31 31 33 33 17 25 26 30 31 30 33 29 29 35 26 32 24 32 23 34] +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 31 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 38 39 + 39 39 39 39 39 39 39 39 39 39 39 38 36 39 39 39 37 39 36 38 36 35 38 35 35 + 35 38 38 37 37 37 35 35 32 33 36 36 36 35 35 34 37 38 36 36 35 33 34 32 36 + 33 36 36 36 36 36 31 36 33 33 36 33 33 36 36 36 36 23 33 31 31 25 27 23 28 + 28 27 28 33 32 32 29 32 31 25 31 23 31] +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 39 37 40 40 39 40 40 39 40 39 39 + 39 40 40 40 39 39 40 39 39 40 40 40 39 40 38 40 39 40 37 39 40 37 39 36 35 + 33 40 35 37 37 36 39 39 38 38 39 39 36 37 36 33 40 36 37 37 34 36 36 35 29 + 36 35 36 33 36 36 35 33 36 36 39 35 33 36 37 36 33 35 38 36 36 35 35 33 37 + 36 35 36 33 34 33 33 33 26 34 33 33 33] +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 37 40 40 40 39 40 39 40 40 39 39 40 39 39 40 40 40 40 40 39 37 + 40 40 38 40 37 35 32 38 40 40 39 37 39 39 36 39 31 40 39 40 40 38 40 39 38 + 38 40 36 39 38 39 36 39 38 39 37 39 38 36 36 36 40 39 39 36 36 36 31 36 36 + 36 36 40 36 37 39 33 36 35 37 38 35 37 38 38 36 36 35 36 36 33 33 16 23 32 + 31 31 31 33 31 23 23 31] +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 38 40 40 40 40 38 40 40 + 40 38 40 40 40 39 40 40 40 40 40 39 39 39 40 40 40 39 40 40 40 39 40 39 39 + 40 27 39 37 40 37 39 36 40 39 36 39 38 38 39 34 33 39 33 38 27 35 35 35 32 + 30 31 32 35 31 23 28 33 32 32 30 27 21 30 32 32 30 30 16 27 27 24 26 17 20 + 19 21 27 30 10 25 31] +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 25 35 38 38 38 38 37 38 40 40 40 40 39 40 40 40 40 39 + 37 40 40 40 35 40 40 40 40 40 39 40 40 40 39 35 40 40 40 34 40 40 40 36 39 + 40 39 40 40 40 39 40 40 40 39 40 40 39 37 36 36 29 40 40 39 39 38 40 38 36 + 29 37 36 36 36 37 39 36 36 38 38 38 38 34 39 36 39 38 38 36 38 35 36 36 38 + 38 31 38 35 35 36 35 38 36 33 36 38 35 33 36 27 36 33 36 33 33 33 34 31 31 + 31] +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 35 40 40 40 40 40 40 36 40 40 40 40 39 + 37 39 40 40 40 39 40 40 40 40 39 37 40 40 40 39 40 40 40 40 40 40 40 36 39 + 38 40 35 40 40 35 40 38 40 37 37 33 40 40 39 36 35 40 36 36 36 37 38 36 39 + 36 40 33 37 34 37 35 36 38 39 38 38 38 39 38 39 38 36 29 36 36 35 36 33 36 + 33 33 35 35 34 34 35 35 34 29 35 34 34 31 34 30 34 33 34 36 31 33 33 33 33 + 29 24 31 27 29 30 26 31 10 26 27 30] +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 + 39 40 40 40 39 40 40 40 40 40 39 40 40 40 40 40 39 39 39 39 40 40 40 39 39 + 40 40 38 40 39 40 40 40 39 39 40 40 40 39 34 40 39 40 37 39 40 37 37 36 36 + 31 39 35 39 38 39 37 38 38 38 38 38 36 38 36 36 39 36 38 39 36 34 38 36 36 + 38 38 39 33 33 35 35 33 33 23 33 31 33 31 31 32] +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 17 38 38 + 35 38 38 40 39 40 40 40 40 39 40 40 40 40 39 40 39 40 40 36 40 40 40 40 37 + 40 40 40 40 40 39 40 40 40 40 40 40 38 40 37 37 35 40 40 40 40 39 40 36 36 + 35 40 36 40 36 37 39 39 33 36 40 34 36 33 35 34 36 33 36 34 35 36 36 36 39 + 36 33 37 33 34 36 33 35 33 28 33 33 35 30 32 32 30] +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 40 40 38 38 38 38 38 40 39 40 40 40 40 39 33 40 40 40 39 + 40 40 40 40 40 40 40 40 40 38 40 40 39 39 39 39 40 40 40 38 40 40 35 40 39 + 40 40 40 40 39 39 40 37 36 40 36 36 36 37 35 34 37 37 38 36 38 35 39 38 38 + 36 38 38 38 38 39 38 36 36 35 38 38 36 36 35 35 38 37 23 36 33 33 34 35 36 + 36 35 33 33 36 31 31 31 33 34 33 34 33 33 31 36 33 33 33 33 31 28 28 19 31 + 30 30 18 28 28 28 24 24 31 16 33 31] +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 38 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 + 35 40 40 40 40 40 40 40 40 40 39 40 40 38 38 36 38 38 40 39 40 40 40 40 40 + 39 40 36 40 40 40 36 40 40 40 39 38 40 40 39 40 40 38 40 38 40 36 40 39 39 + 40 39 39 39 38 36 38 36 34 27 36 39 34 38 38 39 36 37 36 27 36 33 31 33 33 + 36 29 34 34 36 33 29 33 26 14 28 30 28 33 34 33 34 33 29 33 33 29 31 31 33 + 27 31 30 27 23 20 9 24 17 23 20 22 23 28 31 33] +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 37 39 39 39 39 39 + 37 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 36 39 39 39 36 36 + 38 35 38 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 35 38 + 38 39 38 39 39 39 39 37 36 39 38 38 33 39 33 36 39 36 38 39 38 39 38 38 39 + 38 35 39 32 36 39 36 39 33 33 35 33 33 33 33 34 29 33 36 36 36 33 33 32 36 + 32 32 36 36 33 36 32 33 29 27 31 33] +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 36 37 39 39 39 39 39 31 39 39 39 39 39 36 39 39 36 38 36 36 + 36 39 35 39 36 37 39 36 38 39 36 31 36 39 38 38 39 38 34 31 33 23 35 36 36 + 36 34 36 33 36 36 35 23 26 30 28 30 30 35 33 34 35 33 34 34 34 33 35 33 33 + 27 33 33 33 34 36 23 36 18 28 18 28 30] +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 37 39 39 39 39 38 38 + 39 37 39 39 39 39 39 39 39 39 39 39 39 39 38 39 38 39 36 39 39 36 39 35 29 + 34 36 34 38 38 39 39 31 38 38 34 37 38 38 36 38 39 38 33 31 33 33 33 36 36 + 36 36 39 31 33 33 34 34 33 31 33 29 34 36 27 34 31 33 33 36 33 33 32 35 36 + 32 32 29 28 32 31 31 28 25 28 28 30] +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[36 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 35 38 38 38 38 38 38 38 38 38 38 37 38 37 38 38 38 38 35 38 37 38 38 37 + 29 36 38 36 38 36 37 36 35 23 35 38 35 36 36 36 33 38 35 38 33 35 37 35 33 + 35 33 35 36 36 27 33 36 35 31 31 32 31 23 33 26 28 24 29 23 29 10 29 26 26 + 19 24 26 30] +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 39 40 35 40 40 40 40 40 35 40 40 40 40 40 40 40 40 40 40 38 + 35 40 40 40 36 40 40 40 40 40 40 40 40 37 37 37 40 40 40 39 40 40 40 39 36 + 40 40 40 40 38 37 40 40 40 38 40 40 39 40 36 39 36 40 37 35 40 37 40 38 38 + 38 39 36 40 36 35 39 34 38 39 38 39 39 35 36 36 40 36 36 36 36 36 36 38 38 + 38 29 38 35 36 35 33 33 29 32 35 32 35 31 32 26 29 19 27 28 27 24 21 22 25 + 27 27 28 25 30] +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 + 39 38 39 39 39 39 39 39 39 39 36 39 39 38 38 33 38 38 38 39 39 39 39 39 39 + 39 39 39 39 38 39 39 39 39 39 39 38 36 38 37 37 38 37 37 38 37 35 37 36 37 + 39 37 35 39 36 36 39 33 34 17 33 36 34 23 28 30 30 32 33 33 33 31 35 31 31 + 29 33 33 33 33 31 20 31 22 22 28 28 16 34 34 31 33 33] +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 38 40 40 40 40 40 + 35 40 40 40 38 40 40 40 40 40 40 40 39 40 40 40 38 40 40 38 40 40 39 36 38 + 38 38 34 40 40 37 40 40 40 39 40 40 40 40 39 38 39 40 39 39 40 36 39 39 36 + 35 35 39 37 33 40 35 37 37 39 39 37 36 38 38 38 39 38 29 36 36 33 36 33 34 + 36 33 34 31 33 33 33 33 28 21 24 28 11 25 16 23 21 25 36 33 36 33 34 26 26 + 31 25 31] +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 39 40 40 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 38 + 39 39 40 40 40 39 39 40 39 40 40 40 39 40 36 36 36 40 39 40 40 38 40 37 34 + 35 40 33 39 39 40 31 39 39 38 36 35 35 29 33 31 35 33 36 33 33 33 36 36 36 + 33 36 37 34 36 38 36 36 33 27 35 33 33 36 36 33 36 33 31 33 33 27 34 28 33 + 22 31 33 31 34 33 31] +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 35 40 40 40 40 40 40 40 39 40 39 40 40 38 40 40 38 40 40 39 40 40 40 40 40 + 40 39 39 40 33 40 40 40 40 40 40 40 38 40 36 40 39 38 40 40 40 36 39 40 40 + 31 40 38 40 39 37 35 39 40 38 35 40 39 39 37 36 36 40 38 33 33 27 35 35 37 + 37 37 37 36 37 35 36 33 33 35 38 38 33 37 34 36 36 34 35 35 35 31 35 31 37 + 33 33 35 35 26 33 33 32 32 31 35 24 35 25 25 30 18 28 27 21 20 23 20 20 26 + 25 27] +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 38 36 38 38 38 37 38 33 38 38 39 39 39 39 39 39 37 34 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 35 39 36 37 37 37 38 31 36 36 36 37 27 39 37 + 39 39 39 39 35 39 37 23 35 39 33 39 39 39 32 38 39 38 37 37 35 32 35 35 35 + 37 34 33 36 36 35 35 32 34 36 33 36 33 36 33 22 29 36 29 29 8 29 31 36 35 + 34 34 31 33 27 29 25 20 25 21 25 29 21 23 29 15 32 26 24 29 26 25 31 30 27 + 29 30 30 31 33] +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 + 39 39 39 39 38 33 39 39 39 39 39 33 39 39 39 39 39 39 39 37 39 38 39 33 39 + 39 37 38 36 38 36 36 33 36 39 39 39 35 35 35 33 31 38 33 28 30 28 30 36 36 + 36 29 36 33 35 18 28 31 32 32 33 35 35 35 29 33 33 34 32 27 32 28 32] +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 39 40 40 37 40 40 40 38 38 39 40 40 39 40 35 40 39 40 40 38 + 33 40 40 40 36 40 40 40 39 39 36 40 40 39 36 38 39 39 39 36 39 38 40 35 33 + 36 38 33 40 37 36 37 37 36 36 39 36 33 39 36 37 34 39 38 38 39 36 39 36 31 + 33 39 33 37 35 37 36 36 38 36 35 35 36 33 33 33 39 36 31 35 33 33 35 33 36 + 33 33 36 35 36 36 35 33 36 29 32 36 34 35 34 35 33 31 33 33 31 25 34 31 33 + 33 31 33 28 15 28 15 29 30 28 29 31] +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 39 40 37 40 40 40 40 40 39 39 + 40 37 40 40 40 39 38 39 39 40 39 40 40 40 39 39 40 40 37 37 40 34 40 33 23 + 33 35 38 35 31 35 33 32 34 37 36 35 38 34 36 36 33 37 37 37 34 33 33 33 36 + 33 36 33 32 32 26 23 30 29 30 32 32 32 33 33 34 34 31 33 33 29 29 34 31 35 + 33 31 33 31 22 31 31 31 23 18 26 23 31 23 24 27 22 10 28 23 28 7 28 20 30 + 24 30] +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[27 31 31 38 38 38 38 38 38 38 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 35 36 38 38 38 38 38 38 38 38 38 38 38 33 38 38 38 38 38 + 38 35 38 38 38 33 27 33 38 38 38 38 38 38 38 38 38 38 29 27 38 38 38 35 38 + 35 38 38 38 36 38 35 38 38 38 38 38 36 38 37 36 27 35 38 35 35 35 35 38 38 + 38 33 38 35 35 38 35 25 33 33 35 35 35 30 30 25 27 30 38 38 35 33 33 26 33] +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 38 39 40 40 38 38 40 40 40 40 40 39 40 40 40 40 39 + 40 39 40 40 40 39 40 40 40 40 36 40 36 40 37 37 38 38 38 40 37 40 39 40 40 + 39 37 40 39 40 39 35 37 40 40 40 38 36 39 36 39 39 40 39 40 38 38 37 36 38 + 38 27 39 36 38 33 35 29 31 29 32 35 32 36 36 36 31 35 30 32 28 27 28 31 33 + 31 31 31 33 31 33 29 33 33 33 33 34 32 31 31 8 29 31 27 19 29 16 29 31 31 + 29 26 29 32 19 25 31 32] +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 + 35 40 40 40 40 40 40 40 40 40 39 40 40 39 40 40 40 40 40 39 40 40 40 39 35 + 40 37 40 40 40 40 40 40 40 40 40 40 39 40 38 40 38 40 39 39 40 36 40 37 37 + 36 40 36 40 39 40 37 40 38 38 39 38 39 35 39 39 39 38 39 38 33 27 36 36 35 + 39 36 37 38 38 39 33 38 36 35 39 39 39 33 34 33 36 33 36 36 35 31 33 23 31 + 31 31 32 31 33 31 31 31 23 30 18 30] +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 39 + 38 40 40 40 39 40 40 40 40 40 40 40 40 40 39 39 40 40 40 40 40 38 37 40 40 + 40 40 40 40 40 40 40 40 40 40 40 39 38 40 37 37 38 40 37 40 40 37 40 38 39 + 33 40 39 38 40 39 40 38 39 38 38 36 35 35 33 35 33 37 35 29 33 31 31 33 33 + 33 28 33 33 35 33 30 31 33 25 33 33 33 36 36 36 34 33 30 30 32 19 31 19 30 + 28 28 30] +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[28 33 31 38 35 38 38 38 36 38 36 38 38 38 38 38 38 38 38 36 38 38 38 38 38 + 35 38 38 38 38 36 36 36 38 38 38 33 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 31 36 36 33 33 36 30 30 30 35 33 38 38 38 36 38 33 38 38 35 38 38 38 + 38 33 34 30 15 30 27 33 37 38 37 29 33 35 38 35 35 38 34 33 35 35 35 35 35 + 35 35 35 36 38 38 37 35 35 35 32 38 10 7 17 25 18 24 35 33 32 32 32] +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 37 + 37 40 40 40 37 40 40 40 40 40 39 40 40 40 39 36 40 40 40 39 40 40 40 39 39 + 40 37 40 40 40 40 39 40 40 40 39 39 39 40 38 39 39 37 39 40 39 36 40 35 33 + 33 37 38 39 37 39 37 36 39 36 40 40 36 33 35 33 36 36 35 34 35 36 37 28 35 + 33 35 35 34 33 35 28 35 31 33 35 36 33 35 37 34 34 29 33 31 33 19 33 28 31 + 28 29 31 28 33 33 29 23 30] diff -r 000000000000 -r c1bd0c560018 test-data/split_fastq_libraries_sequences.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_fastq_libraries_sequences.fasta Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,172 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG diff -r 000000000000 -r c1bd0c560018 test-data/split_libraries_fastq_histograms.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_histograms.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,8 @@ +Length Count +116.0 20 +126.0 31 +136.0 33 +146.0 2 +156.0 0 +-- + diff -r 000000000000 -r c1bd0c560018 test-data/split_libraries_fastq_seqs.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_seqs.fastq Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,344 @@ +@L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA ++ +ACCCGGGG@G?GGGGGGGGDGGGGGGGGGGGGGGGGDGGBGGGBGGGGGGGDGEGGDDG@GGGGEEEGGGGGG388GD>DDGGEE@DDDDB>FDGD>@-:74=;A*74====?BBD@ +@L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG ++ +GGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHFHBHHECCHBDHFEHAE@@BDB=B@@A2D(5553DAAAAE@EEA>;)9;==A>7B@=@78;:82?C<><9?<>>>98=@ +@L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA ++ +IIIGIIIIGIIIIIGIIHIDIHIHHF@IIFIIIIIGIIIHIHGHBIIIGHHGIFEEEFHHHDEEDG>DBEFBDB@BB=EEBBBEGEDDBA>D@@@@BCBB;BD@@>:@@>B@?=1@@@3<9767=6>;;? +@L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG ++ +IIIIIGIIIIIIIGIIIIIIIIIIIFIIGEGGDIIIIIFFHIIGDGEGD>GDGIIEIGIIFH>GBDDGFGGEGBB2DBDDGBDEBEBB8?B>A@D=38=AC>?>@@?DD@>=?=478;+;BE8>B +@L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIHIIIIIIHEEIIEIIIIIIGGHIHEIIHDFCIGHHHCEDBIEIEEHBBEBBEIHGGGGGDHGGEEGDG?<2A5DBBBBEBEBBB@BEDEBBBE;@@@B@@8:@B@@@@AE@@A0@>=<9:;9=7>:;==DBD=@DBBB=D6'=;;A=?=99=9.9.2,/2===79@@6@ +@L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT ++ +IIIIIIIIGIIIDGIIIFIIHIIIIIIIIIIIIIIEIFGFGGGHIIGIIIHBHIHGIGIHI@EG@EGFFIFEDFBC=;;2?-?=A? +@L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIBIIIGIIIIIIIIIIFIIIIIIIIIIIHGIIIIIIIEDGFGEDDGDBG:@@ECGEFEBHBHFHBB@@GGBBBDBECBBGECBECBFEBDEBEBB@@2B4?<=?;A??A@A+>? +@L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG ++ +IIIIIIIIIIIIHGGIIHIIIIIIIHIIIHIIIIIGIHHIIIIHDIIIHHHDIIIHIIHDHGEHEHGDGHEDGDHEEFEHG,EDCDCF@EBBECB@@B6>@A@ECBBCBBEEA>BBB@@DBAB=@8@@ +@L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHGHHHHGBHHHHHHHHHFHHHHEHHHHEFEHFGGFGAA>C=GGDBDEDEEBCBBEEBB>BD@BB@DBBBB=C>BCCB>EBB8? +@L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGBGGGEGGGG@GGDGHHFGHHHHHHGGFEGBGGG3CE>EAABGDBFFEDFE>E>DCB@EFFBC>EDBDD+CAA>>9?=99DCC?ACABEBBEBEECEBGDECDEEEEEED8B>@@@??EEB*B71;2>7@?===@ +@L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT ++ +IIIIIIIIHIIIHIIIIIIIIIIIIBIIIFIIIIIHIIHHHIIFFIIIFEIFIIIHIIIIIHGIEECHEFIDIBC>GBFFFEEHBEGEBBEFDBCBBF8A??ABBD=B@@DBB??A;;>? +@L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIHIIIIIIIIIHIIIIIGIFIIIIIIIIIHIIIHHIHHFIIIGIIIEGIFHIIGHIIIGHGIGIGHIEDBFAEHHFEGGDGECBC@BDB@ +@L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG ++ +HHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHGHHHHHHHHHGFGGGFGGEGGGGGGHHHHHGHHHHHHHHHEEEGCHHHGGGHHHBHGH>EEDBDEHHHDFBFG>B +@L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIGIIIIIIIIIIHIEIIIHIIIGIIIIIIHIHFIIIIIDIHHIGGIIIIHBHIFIFIIIHHIEE@GBGDDDD@EHEEEECBEEEDCFBCBBFEEI>@B@DBDBEEB9???,?@A=A=<=;*;? +@L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG ++ +IIIIIIIIIIIIGIIIIIIDIIIIIHIIIHIIIIIHIIHHIIIIHIIIHHFHGIHHIGGIEHDICDEIEIIEIBBBD@FBEEBEEEFDDBEFEBBBBEBBBBD?BB88<:<9?B@B@@@A?3@@@9?@ +@L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA ++ +HHHHHHHHDAGGGHHHHHHHHHHHHHHHHHHHHHGHHHHHHH@HHHHGHHFGHHHHHHHFHHHHGHHHDHHGHGGHGBC>DECEDB>6>>>A6>>;4>=6:=9=B:@ +@L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG ++ +GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHHHHHFHHHEHEHHHHHHHBHHEHGHHHFGGGGBEEHEGCBGDCGEGGEGGEGECCEC@EE>B@A==@7,8=>;1=9A>>>;A +@L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +IIIIIIIIIIGIHHIIIIIGIIIIIFIIIHIIIIIHIIIHIIGIGIIIHIIFIIIGIIIDHGDIFIFEHIFBHBDFFFEIE8BDEDDEEFDDHECC@EABCEEBAD3D@BCCCEF>CBBBC8C?B@=@@<<>BB=@@B +@L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG ++ +GGGHHHHHGEGGGHHHHHHHHHHHHHHHHHGHHHHHBHHHHHHGCGBGEEGEEGGDBBADBGDFDEEGCGFEFFCDBBDBECCEFHEEEE:HB;A<::=?>=;EEEAE@>AC>,:.52?;:5255:,7@3:4:@ +@L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIGIIIIIIIIGIIIIIIGIGIIHIIIFIHIIIIHHIIIIIEIEIHIDDGG@GGBGGGHIIIIGIFIGIIHHEEEGBHEHHFFGHGGGGEGDEBEDEACDDCABBDDB@@@BEBEBCCBDBBBBDEE?B@>B +@L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHHHHHBHHHHHHHHHHHHHHHHHHHHHGHHHHHGHHHHHBGHHHHHGHHBGGEGEDGBGGHHH>HDBGDGHHGHED>GB@GEGEGHFBHED@BDDDHFD@C>FCEFEHBB>@?:>??8826=8/:;<<:?>)>A +@L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIIIIIIIIIBHHIIHIHIIIIICIIIFIIIIIHIIIHGHHFEIHIEFIIBIHFHIIHIIGHEHGHGHHGHDBBICHEFHEGGDGEECGHEGDE>GGEDEGDDDBEEBEE@BBEBBDGDC=ABBBB?==-:==? +@L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG ++ +HIIIIIIIIIIIIIIIIIIIIIIIIIDIII3IIGGGGGIIIII@HIIGIIHGIIIIIHIIHIIIHIIDEGGEGGHHIHIHIIGDFIBFGFGGFGFEEGEFEF@EEEEFEBEDFDCD@BBEDE>EDCFBEDBEF>;A>:>AA8>>;AA +@L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ++ +IIIIIIIIIIIIFIHIIIIGIIIIIFIIIHIIIIIIIIIIDIHIIIHIGIIHIIIIIIIIFHHIGHGIHIGEDBBBGBGHFHGCGHGEBEEIEEBEECEEHCHBEFBBB@BBBC>E@B?@@?@=@@BB;>@?>@?>A +@L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG ++ +DGGEBEEE?B,B?GGGGGGGGGGGGGGGGG>GGDGGGGGGGGGGGGGGGGGGGGGA>>G@>GDDGDGFFGD8FBB??>GDGDBA@AD;AAAE5?B;;4ADDBBB@B@D1.?8??-8::;? +@L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIHHHIIIIIIIIHIBIIIFIIIIIHIIIIIIEFIIFIEHFHHIIFIIIIHIHIEIHIHIIFICECIBHHIFEHDEGECBBHEDCBDFCEEBHBBEC>B=BBBCEBDA@BB>B>BB@CB=?@ +@L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIHIIIDIIIIIIIIIIIIFIIIHIIIIIHIIIIHIIIIIIIIIIIIIEIIIIIIIGIHHEIHIHHICEHIHHII@EHEHFEGDGHGEFEEFGGBEHBEEBBBEBEB@@>@=@B +@L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA ++ +IIIIHIHIEGGGEIIIIIIIIIIIIGIIIHIIIEGBGDDDDGGGIIIBEHIIFHHIIFIIGDBC@>EC3@?>@GEGG@@DBD@E8CC=BBDBEAC,38==:;=@?,3=9-<;68<===B +@L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT ++ +HHHHHHHHEGGGGHHHHHHHHHHHHFHHHHHHHDBGGFHHHHBGGDDDHHHHGGHHBFGFCGGFBEGGDGD>D>9ACACEEEBEEBBCDB@EF>DBB@C3*=@AA@=@BAEEBCCB@C +@L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG ++ +IIIIIIIIFIIIIIIIIIIIHIIIHHIIIHIIHIIHIIIFHIIFHIIIHFHIIDHHIHIIIIHIEHDIGIHGFDDCFCB@DEBFEFCFC>EDDBBBBBAAA?A@BB@E@?>-@A@B??@DB-A +@L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIHIIGHIIIFHHIHIGIIIIHIIIIGHHIHIGHIECCGCFFIFFHDFGB=@@DBBBBBBDB?@EBBB=B>=B@BBBB +@L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG ++ +HIIIIIIIIIIIIDIIIHIIIIIIIBIIIGIIIIIGIGIHIFIHHIIIGHIIIFHHIGDFIEEIEFIDFHHGEGBHCFHGEGDGCDEBCCBBEEC>B@B>B8@BBB:B;???9@B>C@>;? +@L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC ++ +IIIIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIHIHHHIIHIHIEIIFEHHIIIIIIIHIGIHIBDHEHGHHHGHHEGDBCDF>DDCBFE@?BB@B@78BB;@=@@?@=;A=????@:>=@=B +@L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIGIIIHIIIIIHIIHHHIIIHIIIEEIFIIIFHIIIHHEIGGGIGIIGIGGDIEGGHGHDEEABCCEDBC@BBEBBDBFEEGABB@BCC<<<;:A0?A +@L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG ++ +IIIIGIIIIIIIIGGIIIIIIIIIIIIIIIIIIDIIIIIIIIHIHHHIIIHFIIHHIGIIIIHGEIDGFIIHIHFEIEHGIGHGGEG@EEBGEEBDEEEDGEHHDGHHGDDCGBDBB?DBD@B??A8A?@DBGGGGGGGDGGGGGGGG@GGGGGGGGGGGGGGGGGGGGGGGDGGDGCFF>?GGGGGGEGGGGGG8GGGGDGGBGCE>CECDEBEC?>,+?A=??==B@BBA<>>/=9?8?>>? +@L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG ++ +IIIIIIIIIIIIIFIIIIIGIIIIIBIIIFIIIGIFIIIHHHHIHIIIFHIGEIIHHIHHIH>IFIEIHHGHIBC>GCFEIEEHGEGDEEDGDBCBBBDDEEH@2@AEEB>BAA>>9>=AA77>>@? +@L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG ++ +BEEGGGGG3EBEDEEEEEGG3@>,EEGGADB??<16/459DFDGFFFDB15*9:/AAAAA(??;;FCCBDDD@>BBDBBB85>;A +@L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG ++ +BBBGGGGGDDGGGACA@,;<58;GDGGGGGGFGGBGGEGGGGGGGGEGEGEGBGEGGGEG8EEFEFEGFBFADCCEBDBFBEEEEAEBBBDD<>A8>>AEEBE@DBB8DB@B0>9)>>@=8@>:3:8@ +@L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +<@BGGGGGGGGGGGGGGGGGGGGGGDGGGGGEEGGGGGGGGGGGGGGGGGEGGGGGGBEGGGGGEGGGGGGGGBG2GBGDGGDEGDGFEGFGEGFFDEEGDEGEFDEGDDBDFC8FCBDDCCDDBFBC5AA>A>A8AAAA5@89? +@L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ++ +IIIIIIIIGIIIHHIIIIIIIIIIIEIIIEIIIIIHIIHHHHHHHHIIGHEHIIIHIHFHICGHEFEIEIHEH@=BD@DEFECB:BBBCBCFCBBC=CCC@8E@CBBCBBBBBD8D@;AA@??BDDEGDBAAD=>@@@ +@L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG ++ +HHHHHHHHDGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHHFEHHHHEHHHHHHHHHHHDHHHHGGHGHGDHHHFGGGHHHHHHFHGHGHGCEE2EBA?CB@B@@BDBBB=B@=BBBBBBD7BBB +@L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG ++ +GGGGGGGGDGGGGHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHGHEHHHHHHHHEHFEFBGGHGGHHBEGEGFHBEHHBHGHHDHHHGHGBHC8>8?ECDEEEECB@/:::;5==>>A +@L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIIIIIHIIIIFGHHHIGIGHHIIIIIIHIIHHIIGFFIHHHGICHGHGHGGHEDHHGDFGGGGGGDB@BCCBDABE@E@BBBBEEBF@?A>?==B@/=?@?>;@BB@ +@L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ++ +IIIIIIIIIIIIIGHIIIIDIIIIIGIIIFIIIGIIIIIIIICGGIIFBDGG@IFFIFHGHIHDBECEHGHGFFD8FCGCGGGGFGEBDEEEBEEGEGBB>BB@BBCCA=;??=A@=?:>@??:@@(@ +@L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC ++ +IIIIIIIIIIIIIIIIIIIDIIIIIHGIIIIIIIIGIIIFIHIIHIIIGIHFIIHIIHIGHGGHEHEBEEEFCGEGDBEECEBBCC<@BDBBEDBDBD@BCBCDDDADB@==@?@B=@@B3B=@@ +@L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG ++ +HHHHHHHHHHDHFHHHHHHHHHHHHGHHHGHHHHHHHHHHHHHHHHHHGGGG>HHHHHEDGDDGFGGGDGGBGEEGBGG8G@DEGGEFEFEBBBDBBD@>?:;??=?B +@L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG ++ +HHHGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHHGG1D>D;A9A8C +@L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ++ +HHHH@HHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHHHHHHHHHGEHHHFHEGEDGDDDGGFFFDDABEEEDDCFGEEDBCAEBEEEEE@EBBEBBEEEE8B@@:<8==<=BAA>A@:@8@ +@L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG ++ +IIIIIIIIIIIIIIHIIIIIIIIIIHIIIIIIIIIIIHIHFIIHIIHIHHHIIIHHIHHIIIHIGIHIFHIFHEDBIDFFEHHGGHHEFEBIEFFCEED>EDEBEEDBEEHDBEFEBDGEEDDBFEDEBCBBB;CBBB +@L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG ++ +IIIIIIIIIIIIIHIIIIIIIIIIIHIIIFIIIHIHIIHHIHHIIIIIHFIIGIFDAGIIHFHHEH@IHIIGIHGGIEHGHEHGHFHGEEEIHHEEE@EEEEIEFHBEDFGDFGGEEDEEBB18A@@@B@88@ +@L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG ++ +IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIHIGIIGIIIIGIIIGIIIHIIIIIHHHIIIHIIIHIHHIIIHHGIGE>FEEEFHEEGGGGCHEHGGEGDEEGG@GDDEDGEBEGDBEEEDEBEBBDDCCDDC>DCC@C?CBCE@BBBB>9@<>?;@+;CCEB>B;/=?=BCBCB>BB>@@B<@?<85*928578=@B +@L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ++ +GGGHGHHHHHHHGHHHHHHFHHHHHFHHHHHHHHHHHHHHHHHHEHHHEEGDGHHHHHHHHHHHGHHHHHHHHDGGHGHHHHFEHGGBHBEHEGHGHGGHGDHAEHEHBBDBBBBC>BEEEBBAEAAEEBEAB><@B +@L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ++ +HHHHHHHHEHHHHHHHHHHGHHHHHHHHHDHHHHHHHHHHHHHHHHHHHHDHHHHEFHHHHH@HHHHHEHHEGEEEHDHEFHEGHE@EHGGHGC@B8DEEECEBEED8;?=??DBCDBCCCBDBBCECGGHH@GGCFGGEGHGB@BBBEEEEH@BBCCB@B>CE=A@@=:==? +@L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG ++ +EEEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGFGFGGGGDGFGGF>EGEGEFED8DGDEEEBGDGBDFDBDBDEE8>+>;;49;? +@L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT ++ +IIIIIHIDIIIIIDIIIIIIIIIIGDIIIEIIIIIIIIFFFIIIHIIIHEIIIIGFIIIGIIHIEHEIFDIFIGGGHEIEDHCGHGHHDEEIEEEEEEGGG>GDEDBB>ADAD@A;>4<=<967:<<=:? +@L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC ++ +HHHHHHHHHHHHHHDHHHHHHHHHHHGHHHHHHHHEHHGGBGGGHHHHHHHHHHGHHHHHHGEGFFGFFGFDFEFHFDHEEHBC2BEC8=??ABBB@D@@>BBBB@5@77==1CC@BB +@L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG ++ +GGGIIIIIIIIIIIHIIIIGIIIIIDIIIGIIIIIIIHIIIGIIGIIHEGGGCIIFIIIHIIIIHGHIHHIEHHEDDHFBIDFFHHFEGGGHG>EEBEBCEBC@BBBB=69=,:186:EBEBC;;@:@ +@L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ++ +IIIIIIIIIIIIGIIIIHIIIIIIHHIIIIIIIIIHIIHHIIIIHIIIHGHHIIIHHIHIIIHIEEEIHIIGIFCDIBHHI@HHGEDD>B@DBEBBBEEEBEFCEGEEBE>>)>@EDCC@B<>:5:6:>68>0A;9>;:@?<>??@B +@L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG ++ +GGGHHHHHHHGHHHHHHHHGHHHHHGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGBHHHHHBHHHHHHHFHGHBHHFGEGEEBEHHHDDDB@GB=?=?EEE>EBD3=@AABDDD>BBCAAECDCDB@BB@:C@BB@B=0=0>?=>@ +@L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ++ +IIIIIIIHIIIIHIIIIIIIIIIIIIIIIIIIIHIIIIIIHIFIIIIIHHIFIIIHGHHIHIIIHHIIFFICIB8BDGD@DBACFEDGCEEBFFFCBBBEBEBAA;8?>?AAABBCC@BB>>C@DB@B@7@@@83;8@89<7+=8=(=5?9? +@L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG ++ +<@@GGGGGGGBGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGBGGGGGGDGGGB@>ADAEEE@D?A=<=@B@@@B@B>BBBBCA@@)>@<4>1>@@>;>A4:@A +@L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ++ +IIIIIIIIIIIIIIIIIIIHIIIIIDIIIIIIIIIHIIHIIIIIHIIIHDIFIIIIIIIIIIHIGIGIHHIEIFFEIEIHIFIGGHGHDHHHGHGBB@@BBB=BBDB?@B:BBBEEECB??A4@4?==? +@L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG ++ +=B@GDGGGEGEGGGGGGGGEGGGGGDGGGGEEEGGGBGGGGGGGDGGGGGGG@EEBBE???DBGGGEGBGGDGGGGBC?0?BDGDDGCBDDDDDDDDEGGFDDDAG+(2:39DBAAA +@L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG ++ +IIIIIIIIHIIIIIIIIIIGIIIIFFIIIFIIIIIHIIIHEIIIHIIIHHIFIIIIHIIIHHHIGHHFHIHEIDBBFGHFHFEHEIIEBDBEEDCDEF=DBDDCBD=D@BDEBDFCC>B@B4B=@=>@=BB>8? diff -r 000000000000 -r c1bd0c560018 test-data/split_libraries_fastq_seqs.fna --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_seqs.fna Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,172 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG diff -r 000000000000 -r c1bd0c560018 test-data/split_libraries_fastq_seqs.qual --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq_seqs.qual Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,584 @@ +>L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[32 34 34 34 38 38 38 38 31 38 30 38 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 35 38 38 33 38 38 38 33 38 38 38 38 38 38 + 38 35 38 36 38 38 35 35 38 31 38 38 38 38 36 36 36 38 38 38 38 38 38 18 23 + 23 38 35 29 35 35 38 38 36 36 31 35 35 35 35 33 29 37 35 38 35 29 31 12 25 + 22 19 28 26 32 9 22 19 28 28 28 28 30 33 33 35 31] +>L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 37 + 39 33 39 39 36 34 34 39 33 35 39 37 36 39 32 36 31 31 33 35 33 28 33 31 31 + 32 17 35 7 20 20 20 18 35 32 32 32 32 36 31 36 36 32 29 26 8 24 26 28 28 + 32 29 22 33 31 28 31 22 23 26 25 23 17 30 34 27 29 27 24 30 27 29 29 29 24 + 23 28 31] +>L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 38 40 40 40 40 38 40 40 40 40 40 38 40 40 39 40 35 40 39 40 39 39 + 37 31 40 40 37 40 40 40 40 40 38 40 40 40 39 40 39 38 39 33 40 40 40 38 39 + 39 38 40 37 36 36 36 37 39 39 39 35 36 36 35 38 29 35 33 36 37 33 35 33 31 + 33 33 28 36 36 33 33 33 36 38 36 35 35 33 32 29 35 31 31 31 31 33 34 33 33 + 26 33 35 31 31 29 25 31 31 29 33 31 30 28 16 31 31 31 18 27 24 22 21 22 28 + 21 29 26 26 30] +>L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 38 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 38 36 38 38 35 40 40 40 40 40 37 37 39 40 40 38 35 38 36 38 35 29 + 38 35 38 40 40 36 40 38 40 40 37 39 29 38 33 35 35 38 37 38 38 36 38 33 33 + 17 35 33 35 35 38 33 35 36 33 36 33 33 23 30 27 33 29 33 29 32 31 35 28 18 + 23 28 32 34 29 30 29 31 31 30 35 35 31 29 28 30 28 19 22 23 26 10 26 33 36 + 23 29 33] +>L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 39 36 36 40 40 36 40 40 40 40 40 40 38 + 38 39 40 39 36 40 40 39 35 37 34 40 38 39 39 39 34 36 35 33 40 36 40 36 36 + 39 33 33 36 33 33 36 40 39 38 38 38 38 38 35 39 38 38 36 36 38 35 38 30 27 + 17 32 20 35 33 33 33 33 36 33 36 33 33 33 31 33 36 35 36 33 33 33 36 26 31 + 31 31 33 31 31 23 25 31 33 31 31 31 31 32 36 31 31 32 15 31 29 28 27 24 25 + 26 24 28 22 29 25 26 28 28 27 30] +>L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 38 38 36 38 36 38 38 38 38 38 39 39 39 39 39 39 39 38 39 39 38 38 + 34 38 38 39 37 39 39 39 39 39 39 39 39 36 35 33 38 38 39 37 39 39 39 39 37 + 39 39 39 39 39 37 39 39 39 39 39 36 33 37 34 36 36 35 37 37 37 35 36 28 26 + 31 34 28 34 34 34 32 32 33 33 35 31 31 25 28 26 30 28 33 33 31 29 35 33 35 + 28 31 35 33 33 33 28 35 21 6 28 26 26 32 28 30 28 24 24 28 24 13 24 13 17 + 11 14 17 28 28 28 22 24 31 31 21 31] +>L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 35 38 40 40 40 37 40 40 39 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 36 40 37 38 37 38 38 38 39 40 40 38 40 40 40 + 39 33 39 40 39 38 40 38 40 39 40 31 36 38 31 36 38 37 37 40 37 36 35 37 33 + 34 27 37 36 34 31 33 10 31 33 33 33 33 23 17 28 28 30 33 33 31 27 33 33 31 + 33 33 8 32 32 31 23 28 31 30 31 31 20 30 27 29 28 26 26 17 30 12 30 28 32 + 30] +>L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 + 40 40 39 38 40 40 40 40 40 40 40 36 35 38 37 38 36 35 35 38 35 33 38 25 31 + 31 36 34 38 36 37 36 33 39 33 39 37 39 33 33 31 31 38 38 33 33 33 35 33 36 + 34 33 33 38 36 34 33 36 34 33 37 36 33 35 36 33 36 33 33 31 31 17 33 19 30 + 27 28 30 26 32 30 30 32 31 32 10 29 30] +>L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 38 38 40 40 39 40 40 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 38 40 39 39 40 40 40 40 39 35 40 40 40 39 39 + 39 35 40 40 40 39 40 40 39 35 39 38 36 39 36 39 38 35 38 39 36 35 38 35 39 + 36 36 37 36 39 38 11 36 35 34 35 34 37 31 36 33 33 36 34 33 31 31 33 21 29 + 31 32 31 36 34 33 33 34 33 33 36 36 32 29 33 33 33 31 31 35 33 32 33 28 31 + 23 31 31] +>L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 38 39 39 39 39 38 33 39 39 39 39 39 + 39 39 39 39 37 39 39 39 39 36 39 39 39 39 36 37 36 39 37 38 38 37 38 32 32 + 29 34 28 38 38 35 33 35 36 35 36 36 33 34 33 33 36 36 33 33 29 33 35 31 33 + 33 31 35 33 33 33 33 28 34 29 33 34 34 33 29 36 33 33 23 30] +>L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 38 33 38 38 38 36 38 38 38 38 31 38 38 35 38 39 39 + 37 38 39 39 39 39 39 39 38 38 37 36 38 33 38 38 38 18 34 36 29 36 32 32 33 + 38 35 33 37 37 36 35 37 36 29 36 29 35 34 33 31 36 37 37 33 34 29 36 35 33 + 35 35 10 34 32 32 29 29 24 30 28 24 24 35 34 34 30 32 34 32 33 36 33 33 36 + 33 27 33 33 35 31 33 31 31 31 33] +>L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 + 40 40 40 40 40 40 40 40 40 40 39 40 40 38 40 40 38 40 40 40 40 39 40 40 40 + 40 35 38 40 33 37 39 35 39 33 36 39 39 40 36 35 39 38 39 39 40 37 37 38 38 + 39 31 29 36 36 34 36 33 38 35 36 34 35 36 36 36 36 36 36 35 23 33 29 31 31 + 31 30 30 36 36 33 9 33 22 16 26 17 29 22 31 30 28 28 28 31] +>L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 39 39 39 40 40 37 37 40 40 40 37 36 + 40 37 40 40 40 39 40 40 40 40 40 39 38 40 36 36 34 39 36 37 40 35 40 33 34 + 29 38 33 37 37 37 36 36 39 33 36 38 36 33 33 36 37 35 33 34 33 33 37 23 32 + 30 30 32 33 33 35 28 33 31 31 35 33 33 30 30 32 26 26 29 30] +>L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 38 40 + 37 40 40 40 40 40 40 40 40 40 39 40 40 40 39 39 40 39 39 37 40 40 40 38 40 + 40 40 36 38 40 37 39 40 40 38 39 40 40 40 38 39 38 40 38 40 38 39 40 36 35 + 33 37 32 36 39 39 37 36 38 38 35 38 36 27 34 33 37 34 38 35 36 38 38 38 38 + 36 38 39 36 36 34 36 29 34 33 34 31 33 35 33 31] +>L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 37 38 38 38 37 38 + 38 36 38 38 38 38 38 38 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 36 36 + 36 38 34 39 39 39 38 38 38 39 39 39 33 39 38 39 29 36 36 35 33 35 36 39 39 + 39 35 37 33 37 38 29 27 30 30 34 32 34 23 35 35 32 33 35 36 36 33 36 35 33 + 31 33 36 33 31 33 29 33] +>L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 40 + 36 40 40 40 39 40 40 40 38 40 40 40 40 40 40 39 40 39 37 40 40 40 40 40 35 + 40 39 39 40 38 38 40 40 40 40 39 33 39 40 37 40 37 40 40 40 39 39 40 36 36 + 31 38 33 38 35 35 35 35 31 36 39 36 36 36 36 34 33 36 36 36 35 34 37 33 34 + 33 33 37 36 36 40 29 31 33 31 35 33 35 33 36 36 33 24 30 30 30 11 30 27 32 + 29 31 32 28 32 28 27 28 26 9 26 30] +>L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 35 40 40 40 40 40 + 39 40 40 40 39 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 39 + 37 39 38 40 39 39 40 38 38 40 36 39 35 40 34 35 36 40 36 40 40 36 40 33 33 + 33 35 31 37 33 36 36 33 36 36 36 37 35 35 33 36 37 36 33 33 33 33 36 33 33 + 33 33 35 30 33 33 23 23 27 25 27 24 30 33 31 33 31 31 31 32 30 18 31 31 31 + 24 30 31] +>L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 32 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 31 39 39 39 39 38 39 39 + 37 38 39 39 39 39 39 39 39 37 39 39 39 39 38 39 39 39 35 39 39 38 39 38 38 + 39 27 38 36 31 35 36 33 36 37 36 37 39 38 38 38 33 38 31 33 33 36 35 33 35 + 33 31 35 36 33 37 36 36 38 36 36 29 38 33 34 29 35 36 34 36 35 33 29 21 29 + 29 29 32 21 29 29 26 19 29 28 21 25 28 24 28 33 25 31] +>L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 36 39 39 39 39 39 39 39 37 39 39 39 36 39 36 39 39 39 39 39 39 39 33 + 39 39 36 39 38 39 39 39 37 38 38 38 38 33 36 36 39 36 38 34 33 38 35 34 38 + 36 38 38 36 38 38 36 38 36 34 34 36 34 31 36 36 29 33 31 32 28 28 31 22 11 + 23 28 29 26 16 28 24 32 29 29 29 26 32] +>L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 38 40 39 39 40 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 39 40 40 38 40 38 40 40 40 39 40 + 40 37 40 40 40 38 40 40 40 35 39 38 35 40 37 40 37 36 39 40 37 33 39 33 35 + 37 37 37 36 40 36 23 33 35 36 35 35 36 36 37 35 35 39 36 34 34 31 36 32 33 + 34 36 36 33 32 35 18 35 31 33 34 34 34 36 37 29 34 33 33 33 34 23 34 30 33 + 31 28 31 31 27 27 29 33 33 28 31 31 33] +>L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[38 38 38 39 39 39 39 39 38 36 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 33 39 39 39 39 39 39 38 34 38 33 38 36 36 + 38 36 36 38 38 35 33 33 32 35 33 38 35 37 35 36 36 38 34 38 37 36 37 37 34 + 35 33 33 35 33 36 34 34 36 37 39 36 36 36 36 25 39 33 26 32 27 25 25 28 30 + 29 28 26 36 36 36 32 36 31 29 32 34 29 11 25 13 20 17 30 26 25 20 17 20 20 + 25 11 22 31 18 25 19 25 31] +>L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 38 40 38 40 40 39 + 40 40 40 37 40 39 40 40 40 40 39 39 40 40 40 40 40 36 40 36 40 39 40 35 35 + 38 38 31 38 38 33 38 38 38 39 40 40 40 40 38 40 37 40 38 40 40 39 39 36 36 + 36 38 33 39 36 39 39 37 37 38 39 38 38 38 38 36 38 35 36 33 36 35 36 32 34 + 35 35 34 32 33 33 35 35 33 31 31 31 33 36 33 36 33 34 34 33 35 33 33 33 33 + 35 36 36 30 33 31 29 33] +>L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[38 38 38 39 39 39 39 39 33 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 38 39 39 39 39 39 33 38 39 39 39 39 39 38 + 39 39 33 38 38 36 38 36 35 38 33 38 38 39 39 39 29 39 35 33 38 35 38 39 39 + 38 39 36 35 29 38 33 31 38 36 38 36 38 39 37 33 39 36 35 31 33 35 35 35 39 + 37 35 31 34 29 37 34 36 37 36 39 33 33 29 31 30 25 29 30 30 23 23 17 21 28 + 23 14 25 26 27 27 25 30 29 8 29 32] +>L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 33 39 39 40 40 39 40 39 40 40 40 40 40 + 34 40 40 40 37 40 40 40 40 40 39 40 40 40 39 38 39 39 37 36 40 39 40 36 37 + 40 40 33 40 39 37 39 40 40 39 40 40 38 39 36 39 38 39 38 39 39 38 39 35 33 + 33 40 34 39 36 37 39 36 38 38 35 38 36 36 34 38 39 36 38 35 36 29 38 38 36 + 35 36 38 35 35 35 33 36 36 33 36 36 31 33 33 36 33 33 35 38 35 34 28 32 33 + 33 33 33 30 28 28 12 25 28 28 30] +>L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 40 35 40 40 40 18 40 40 38 38 38 38 38 40 40 40 40 40 31 39 40 40 38 40 40 + 39 38 40 40 40 40 40 39 40 40 39 40 40 40 39 40 40 35 36 38 38 36 38 38 39 + 39 40 39 40 39 40 40 38 35 37 40 33 37 27 35 33 35 35 33 40 35 39 39 40 40 + 38 38 38 37 40 37 36 39 32 32 34 36 32 36 40 35 40 24 40 40 37 34 37 37 36 + 36 36 37 27 33 33 27 35 28 28 30 30 30 27 31] +>L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 38 40 39 39 39 39 39 39 40 39 40 39 40 35 36 + 40 38 34 40 37 39 39 40 39 36 40 39 36 39 36 39 36 40 36 40 39 36 40 33 35 + 33 37 33 37 34 35 35 35 38 36 38 36 36 35 35 35 38 34 33 35 33 33 35 32 33 + 36 33 36 35 38 35 36 32 36 33 34 33 34 33 33 36 33 33 31 36 33 22 33 33 31 + 33 33 33 21 30] +>L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[34 34 34 38 33 38 38 38 36 38 38 38 38 38 38 38 36 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 35 38 38 37 38 38 38 38 38 38 38 38 37 35 35 38 37 38 + 38 36 38 38 38 33 36 38 36 38 35 38 38 38 37 38 29 38 37 38 38 37 38 37 36 + 36 38 36 37 36 37 31 36 36 36 36 37 36 33 36 35 37 35 34 35 31 33 33 36 35 + 36 29 36 35 34 37 33 36 35 33 36 37 29 26 32 29 25 29 32 32 23 29 29 26 32 + 32] +>L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 37 40 39 40 40 40 40 38 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 40 40 40 40 40 35 40 39 40 40 40 39 40 38 40 + 40 39 40 40 40 40 40 40 40 40 37 39 39 40 38 39 38 40 39 40 38 36 35 33 33 + 33 38 33 38 39 37 39 38 34 38 39 38 36 33 36 36 40 36 36 33 36 36 34 36 36 + 39 34 39 33 36 37 33 33 33 31 33 33 33 34 29 36 31 33 30 31 31 30 31 28 31 + 31 33 33 26 29 31 30 29 31 30 29 32] +>L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[35 38 38 36 33 36 36 36 30 33 11 33 30 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 29 38 38 35 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 32 29 29 38 31 29 38 27 36 30 33 36 36 31 38 38 35 38 38 35 + 38 33 35 38 36 33 29 35 35 38 35 38 37 37 38 35 23 37 33 33 30 30 29 38 35 + 38 35 33 32 31 32 35 26 32 32 32 36 20 30 33 26 26 19 32 35 35 33 33 33 31 + 33 31 35 16 13 30 23 30 30 12 23 25 25 26 30] +>L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 39 40 + 33 40 40 40 37 40 40 40 40 40 39 40 40 40 40 40 40 36 37 40 40 37 40 36 39 + 37 39 39 40 40 37 40 40 40 40 39 40 39 40 36 40 39 40 39 40 40 37 40 34 36 + 34 40 33 39 39 40 37 36 39 35 36 38 36 34 33 33 39 36 35 34 33 35 37 34 36 + 36 33 39 33 33 36 34 29 33 28 33 33 33 34 36 33 35 32 31 33 33 29 33 29 33 + 33 31 34 33 28 30 31] +>L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 35 40 40 40 40 40 40 40 40 40 40 40 40 + 37 40 40 40 39 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 + 40 40 40 40 36 40 40 40 40 40 40 40 38 40 39 39 36 40 39 40 39 39 40 34 36 + 39 40 39 39 40 40 31 36 39 36 39 37 36 38 35 38 39 38 36 37 36 36 37 38 38 + 33 36 39 33 36 36 27 37 31 31 33 35 35 35 34 33 35 33 35 35 33 33 33 29 33 + 33 33 36 33 36 33 31 31 29 31 28 31 33] +>L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 39 40 36 38 38 38 36 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 36 38 33 38 35 35 35 35 38 38 38 40 40 40 33 36 39 + 40 40 37 39 39 40 40 37 40 40 38 35 33 34 31 29 36 34 18 31 30 29 31 38 36 + 38 38 31 31 35 33 35 31 36 23 34 34 28 33 33 35 33 36 32 34 11 18 23 28 28 + 25 26 28 31 30 11 18 28 24 12 27 26 27 30 32 30 30] +>L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 + 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 36 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 38 36 33 36 36 38 36 38 38 37 38 34 34 + 33 38 31 36 36 37 35 36 36 36 38 35 36 36 33 33 39 36 35 36 33 36 36 38 38 + 33 36 38 33 32 36 33 35 31 32 39 35 34 30 34 36 31 30 33 35 33 25 29 21 23 + 27 28 28 28 33] +>L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 37 39 39 39 39 39 39 39 35 33 38 38 37 39 39 39 39 33 38 38 35 35 35 39 39 + 39 39 38 38 39 39 33 37 38 37 34 38 38 37 33 36 38 38 35 38 35 29 35 29 24 + 32 34 32 34 36 36 36 33 36 36 33 33 34 35 33 31 36 37 29 35 33 33 31 34 18 + 9 28 31 32 32 31 28 31 33 32 36 36 33 34 34 33 31 34] +>L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 39 + 39 40 40 40 39 40 40 39 40 40 39 40 40 40 37 39 40 40 37 39 40 40 40 39 37 + 39 40 40 35 39 39 40 39 40 40 40 40 39 40 36 39 35 40 38 40 39 38 37 35 35 + 34 37 34 33 31 35 36 33 37 36 37 34 37 34 29 36 35 35 33 33 33 33 33 32 32 + 32 30 32 31 33 33 31 36 31 30 29 12 31 32 31 33 30 30 31 35 33 12 32] +>L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 38 39 40 40 40 37 39 + 39 40 39 40 38 40 40 40 40 39 40 40 40 40 38 39 39 40 39 40 38 39 40 36 34 + 34 38 34 37 37 40 37 37 39 35 37 38 33 28 31 31 35 33 33 33 33 33 33 35 33 + 30 31 36 33 33 33 28 33 29 28 33 31 33 33 33 33] +>L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 39 40 40 40 40 40 40 40 + 33 40 40 40 38 40 40 40 40 40 38 40 38 40 39 40 37 40 39 39 40 40 40 38 39 + 40 40 40 37 39 39 40 38 35 37 40 36 36 40 36 37 40 35 37 39 39 38 36 38 33 + 39 34 37 39 38 36 38 35 38 34 35 36 33 34 34 33 33 36 36 34 29 33 31 33 29 + 33 23 31 33 33 33 25 33 26 30 30 30 24 31 33 29 34 31 29 26 30] +>L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 39 40 39 40 36 40 + 40 37 36 39 39 40 40 40 40 40 40 40 39 40 38 40 39 40 33 35 39 36 39 38 39 + 39 39 38 39 39 36 38 35 33 34 35 37 29 35 35 34 33 37 36 31 30 33 33 31 33 + 31 22 23 33 33 26 31 28 31 31 30 31 28 26 32 28 30 30 30 30 31 25 29 28 31 + 28 33] +>L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 38 40 40 40 39 40 40 40 40 40 39 40 40 39 39 39 40 40 40 39 40 40 40 36 36 + 40 37 40 40 40 37 39 40 40 40 39 39 36 40 38 38 38 40 38 40 40 38 40 38 38 + 35 40 36 38 38 39 38 39 35 36 36 32 33 34 34 36 35 33 34 31 33 33 36 33 33 + 35 33 37 36 36 38 32 33 33 31 33 34 34 27 27 30 6 10 28 29 27 27 26 25 32 + 15 30 32] +>L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 38 40 40 40 40 40 40 40 40 38 38 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 35 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 40 + 39 37 40 40 39 39 40 38 40 40 40 40 39 38 36 40 35 38 37 40 40 39 40 39 37 + 36 40 36 39 38 40 38 39 38 38 36 38 31 36 36 33 38 36 36 33 35 36 36 36 35 + 38 36 39 39 35 38 39 39 38 35 35 34 38 33 35 33 33 30 35 33 35 31 33 30 30 + 32 23 32 30 31 35 27 33 22 12 30] +>L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[32 29 33 38 38 38 38 38 38 38 35 38 38 38 38 38 38 38 38 31 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 35 38 38 35 38 34 37 + 37 29 30 38 38 38 38 38 38 36 38 38 38 38 38 38 23 38 38 38 38 35 38 38 33 + 38 27 38 36 38 27 38 38 36 35 36 38 38 38 38 36 36 38 16 28 30 30 33 36 37 + 35 29 34 36 29 34 36 34 35 36 33 36 34 30 29 11 10 30 32 28 30 30 28 28 33 + 31 33 33 32 27 29 29 14 28 24 30 23 30 29 29 30] +>L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 38 40 40 40 40 40 + 33 40 40 40 37 40 40 40 38 40 37 40 40 40 39 39 39 39 40 39 40 40 40 37 39 + 40 38 36 40 40 39 39 40 39 39 40 39 29 40 37 40 36 40 39 39 38 39 40 33 34 + 29 38 34 37 36 40 36 36 39 38 36 38 35 36 36 35 38 35 33 34 33 33 33 35 35 + 36 36 39 31 27 33 33 33 36 33 34 33 36 36 37 36 36 33 36 36 36 31 32 25 30 + 31 33 33 27 20 23 6 17 27 27 27 21 24 26 30 32] +>L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[36 37 37 38 38 38 38 38 38 33 35 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 33 38 + 36 38 38 36 38 31 38 38 38 38 36 37 37 38 36 34 38 38 34 34 36 32 34 35 35 + 35 23 38 26 31 32 31 31 31 33 35 35 33 37 36 39 36 35 33 29 17 31 32 36 36 + 33 29 33 32 32 29 29 24 29 28 32 32 22 22 29 29 31 30] +>L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[33 36 36 38 38 38 38 38 18 36 33 36 35 36 36 36 36 36 38 38 18 27 38 36 36 + 35 27 36 38 38 31 36 35 38 38 33 27 38 36 36 36 36 32 33 13 19 33 33 20 29 + 31 29 11 36 36 27 36 36 38 38 38 38 35 38 37 38 38 38 29 38 38 32 35 33 30 + 30 27 16 21 14 19 20 24 35 37 35 38 37 37 37 35 33 16 20 9 24 25 14 32 32 + 32 32 32 7 30 30 26 26 37 34 34 33 35 35 35 31 29 33 33 35 33 33 33 23 20 + 29 26 32] +>L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[33 33 33 38 38 38 38 38 35 35 38 38 38 32 34 32 31 11 26 27 20 23 26 38 35 + 38 38 38 38 38 38 37 38 38 33 38 38 36 38 38 38 38 38 38 38 38 36 38 36 38 + 36 38 33 38 36 38 38 38 36 38 23 36 36 37 36 37 36 38 37 33 37 32 35 34 34 + 36 33 35 33 37 33 36 36 36 36 32 36 33 33 33 35 35 27 29 32 23 29 29 32 36 + 36 33 36 31 35 33 33 23 35 33 31 33 15 29 24 8 29 29 31 28 23 31 29 25 18 + 25 23 31] +>L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[27 31 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 35 38 38 38 38 38 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 36 38 38 38 38 38 38 33 36 38 38 38 38 38 36 38 38 38 38 38 38 38 38 33 38 + 17 38 33 38 35 38 38 35 36 38 35 38 37 36 38 37 38 36 38 37 37 35 36 36 38 + 35 36 38 36 37 35 36 38 35 35 33 35 37 34 23 37 34 33 35 35 34 34 35 35 33 + 37 33 34 20 32 32 29 32 29 32 27 32 32] +>L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 + 40 39 40 40 40 40 31 40 40 40 35 40 40 40 40 40 40 40 36 39 40 39 40 39 40 + 40 39 40 40 40 40 40 37 39 40 40 39 38 40 38 39 36 40 40 40 37 39 40 38 39 + 38 35 39 39 39 34 38 36 34 34 35 36 38 39 35 36 35 38 36 33 33 33 36 35 38 + 35 33 38 34 34 34 30 32 31 28 33 31 31 23 33 31 33] +>L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 36 39 34 35 + 31 38 35 39 33 39 39 39 39 36 38 38 36 36 32 34 36 31 36 34 36 34 34 33 35 + 33 27 35 28 35 31 31 32 29 23 32 32 32 32 20 31 23 24 30] +>L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 38 40 40 40 39 39 40 40 40 40 40 40 40 40 40 40 40 + 36 40 40 40 36 40 40 40 40 40 39 40 40 39 39 39 39 39 39 39 39 40 40 38 39 + 36 39 40 40 40 39 40 39 37 39 40 34 38 39 36 37 36 40 36 40 39 36 39 31 28 + 33 35 31 35 36 37 36 34 33 25 33 33 33 34 33 34 37 34 33 33 34 28 34 34 34 + 31 23 36 31 34 33 33 34 33 33 33 33 33 35 23 35 31 26 32 32 31 30 30 33 35 + 35 36 38 35 33 32 32 35 28 29 31 31 31] +>L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 39 39 39 39 39 35 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 37 36 + 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 38 38 39 38 + 39 38 35 39 39 39 37 38 38 38 39 39 39 39 39 39 37 39 38 39 38 39 38 34 36 + 36 17 36 33 27 32 35 31 36 36 36 33 33 36 38 32 38 36 34 36 33 33 23 16 30 + 26 30 30] +>L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 39 40 38 + 39 40 40 40 39 40 40 40 40 40 39 40 40 38 39 40 40 40 40 39 40 40 40 39 40 + 40 38 40 40 40 39 40 39 40 40 40 39 39 40 38 40 39 40 39 40 40 38 40 35 34 + 33 40 36 37 39 39 37 39 35 38 39 38 36 35 33 36 38 34 36 35 34 35 36 34 33 + 33 33 36 31 35 35 31 33 32 29 32 30 34 33 31 33 31 31 33 35 33 33 33 28 33 + 31 28 33 33 33 33 33 33 35 22 33 33 33] +>L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 38 38 38 38 38 35 38 38 38 38 39 39 39 39 39 39 37 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 36 39 39 39 + 39 39 39 39 39 36 39 37 36 37 33 38 38 39 38 38 39 39 33 36 38 36 38 37 39 + 33 36 39 39 33 39 38 39 39 35 39 39 39 38 39 38 33 39 34 23 29 23 30 36 34 + 35 36 36 36 36 34 27 33 34 36 36 33 31 24 16 26 28 28 33 33 25 33 31 33 33 + 29 33 31 14 25 25 25 26 20 28 28 29 29 32] +>L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 37 38 39 39 39 40 38 40 38 39 + 39 40 40 40 40 40 40 39 40 40 39 39 40 40 38 37 37 40 39 39 39 38 40 34 39 + 38 39 38 39 38 38 39 36 35 39 39 38 35 37 38 38 38 38 38 38 35 33 31 33 34 + 34 33 35 32 33 36 31 36 31 33 33 33 33 36 36 33 37 31 30 32 29 30 28 28 33 + 31 14 28 30 31 30 29 26 31 33 33 31] +>L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 38 39 40 40 40 40 35 40 40 40 40 40 + 38 40 40 40 37 40 40 40 38 40 40 40 40 40 40 40 40 34 38 38 40 40 37 33 35 + 38 38 31 40 37 37 40 37 39 38 39 40 39 35 33 36 34 36 39 38 39 38 37 37 35 + 23 37 34 38 34 38 38 38 38 37 38 36 33 35 36 36 36 33 36 36 38 36 38 33 33 + 29 33 33 31 33 33 34 34 32 28 26 30 30 28 27 30 26 19 29 32 31 28 30 25 29 + 31 30 30 25 31 31 7 31] +>L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 40 40 + 39 38 40 40 40 40 40 40 40 40 38 40 40 40 37 40 39 40 40 39 40 40 40 38 40 + 39 37 40 40 39 40 40 39 40 38 39 38 38 39 36 39 27 39 38 39 38 36 37 33 34 + 29 36 33 36 36 36 37 34 38 36 38 35 33 36 36 34 36 33 33 34 34 27 31 33 35 + 33 33 36 35 33 35 33 35 31 33 34 33 34 35 35 35 32 35 33 31 28 28 31 30 31 + 33 28 31 31 33 18 33 28 31 31] +>L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 35 39 37 39 39 39 39 39 39 39 39 39 39 39 39 + 38 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 38 + 38 38 29 39 39 39 39 39 36 35 38 35 35 38 37 38 38 38 35 38 38 33 38 36 36 + 38 33 38 38 23 38 31 35 36 38 38 36 37 36 37 36 27 38 38 36 36 37 36 38 38 + 39 38 18 35 38 33 33 33 35 35 38 35 31 38 37 35 36 36 35 33 35 33 33 29 33 + 33 33 35 33 33 35 31 29 30 25 26 30 30 28 30 33] +>L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[39 39 39 38 38 38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 + 39 39 39 38 38 16 35 27 30 32 26 28 26 38 37 38 33 38 38 27 38 35 38 35 35 + 38 38 33 32 32 35 38 31 39 39 33 39 37 38 39 36 38 36 27 30 28 23 23 35 35 + 35 32 35 31 31 33 33 17 25 26 30 31 30 33 29 29 35 26 32 24 32 23 34] +>L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[39 39 39 39 31 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 38 39 + 39 39 39 39 39 39 39 39 39 39 39 38 36 39 39 39 37 39 36 38 36 35 38 35 35 + 35 38 38 37 37 37 35 35 32 33 36 36 36 35 35 34 37 38 36 36 35 33 34 32 36 + 33 36 36 36 36 36 31 36 33 33 36 33 33 36 36 36 36 23 33 31 31 25 27 23 28 + 28 27 28 33 32 32 29 32 31 25 31 23 31] +>L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 39 37 40 40 39 40 40 39 40 39 39 + 39 40 40 40 39 39 40 39 39 40 40 40 39 40 38 40 39 40 37 39 40 37 39 36 35 + 33 40 35 37 37 36 39 39 38 38 39 39 36 37 36 33 40 36 37 37 34 36 36 35 29 + 36 35 36 33 36 36 35 33 36 36 39 35 33 36 37 36 33 35 38 36 36 35 35 33 37 + 36 35 36 33 34 33 33 33 26 34 33 33 33] +>L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 37 40 40 40 39 40 39 40 40 39 39 40 39 39 40 40 40 40 40 39 37 + 40 40 38 40 37 35 32 38 40 40 39 37 39 39 36 39 31 40 39 40 40 38 40 39 38 + 38 40 36 39 38 39 36 39 38 39 37 39 38 36 36 36 40 39 39 36 36 36 31 36 36 + 36 36 40 36 37 39 33 36 35 37 38 35 37 38 38 36 36 35 36 36 33 33 16 23 32 + 31 31 31 33 31 23 23 31] +>L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 39 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 38 40 40 40 40 38 40 40 + 40 38 40 40 40 39 40 40 40 40 40 39 39 39 40 40 40 39 40 40 40 39 40 39 39 + 40 27 39 37 40 37 39 36 40 39 36 39 38 38 39 34 33 39 33 38 27 35 35 35 32 + 30 31 32 35 31 23 28 33 32 32 30 27 21 30 32 32 30 30 16 27 27 24 26 17 20 + 19 21 27 30 10 25 31] +>L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 25 35 38 38 38 38 37 38 40 40 40 40 39 40 40 40 40 39 + 37 40 40 40 35 40 40 40 40 40 39 40 40 40 39 35 40 40 40 34 40 40 40 36 39 + 40 39 40 40 40 39 40 40 40 39 40 40 39 37 36 36 29 40 40 39 39 38 40 38 36 + 29 37 36 36 36 37 39 36 36 38 38 38 38 34 39 36 39 38 38 36 38 35 36 36 38 + 38 31 38 35 35 36 35 38 36 33 36 38 35 33 36 27 36 33 36 33 33 33 34 31 31 + 31] +>L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 39 40 40 35 40 40 40 40 40 40 36 40 40 40 40 39 + 37 39 40 40 40 39 40 40 40 40 39 37 40 40 40 39 40 40 40 40 40 40 40 36 39 + 38 40 35 40 40 35 40 38 40 37 37 33 40 40 39 36 35 40 36 36 36 37 38 36 39 + 36 40 33 37 34 37 35 36 38 39 38 38 38 39 38 39 38 36 29 36 36 35 36 33 36 + 33 33 35 35 34 34 35 35 34 29 35 34 34 31 34 30 34 33 34 36 31 33 33 33 33 + 29 24 31 27 29 30 26 31 10 26 27 30] +>L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 + 39 40 40 40 39 40 40 40 40 40 39 40 40 40 40 40 39 39 39 39 40 40 40 39 39 + 40 40 38 40 39 40 40 40 39 39 40 40 40 39 34 40 39 40 37 39 40 37 37 36 36 + 31 39 35 39 38 39 37 38 38 38 38 38 36 38 36 36 39 36 38 39 36 34 38 36 36 + 38 38 39 33 33 35 35 33 33 23 33 31 33 31 31 32] +>L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 17 38 38 + 35 38 38 40 39 40 40 40 40 39 40 40 40 40 39 40 39 40 40 36 40 40 40 40 37 + 40 40 40 40 40 39 40 40 40 40 40 40 38 40 37 37 35 40 40 40 40 39 40 36 36 + 35 40 36 40 36 37 39 39 33 36 40 34 36 33 35 34 36 33 36 34 35 36 36 36 39 + 36 33 37 33 34 36 33 35 33 28 33 33 35 30 32 32 30] +>L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 39 40 40 40 38 38 38 38 38 40 39 40 40 40 40 39 33 40 40 40 39 + 40 40 40 40 40 40 40 40 40 38 40 40 39 39 39 39 40 40 40 38 40 40 35 40 39 + 40 40 40 40 39 39 40 37 36 40 36 36 36 37 35 34 37 37 38 36 38 35 39 38 38 + 36 38 38 38 38 39 38 36 36 35 38 38 36 36 35 35 38 37 23 36 33 33 34 35 36 + 36 35 33 33 36 31 31 31 33 34 33 34 33 33 31 36 33 33 33 33 31 28 28 19 31 + 30 30 18 28 28 28 24 24 31 16 33 31] +>L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 40 40 40 40 38 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 + 35 40 40 40 40 40 40 40 40 40 39 40 40 38 38 36 38 38 40 39 40 40 40 40 40 + 39 40 36 40 40 40 36 40 40 40 39 38 40 40 39 40 40 38 40 38 40 36 40 39 39 + 40 39 39 39 38 36 38 36 34 27 36 39 34 38 38 39 36 37 36 27 36 33 31 33 33 + 36 29 34 34 36 33 29 33 26 14 28 30 28 33 34 33 34 33 29 33 33 29 31 31 33 + 27 31 30 27 23 20 9 24 17 23 20 22 23 28 31 33] +>L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 37 39 39 39 39 39 + 37 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 36 39 39 39 36 36 + 38 35 38 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 35 38 + 38 39 38 39 39 39 39 37 36 39 38 38 33 39 33 36 39 36 38 39 38 39 38 38 39 + 38 35 39 32 36 39 36 39 33 33 35 33 33 33 33 34 29 33 36 36 36 33 33 32 36 + 32 32 36 36 33 36 32 33 29 27 31 33] +>L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[39 39 39 39 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 35 39 39 39 39 36 37 39 39 39 39 39 31 39 39 39 39 39 36 39 39 36 38 36 36 + 36 39 35 39 36 37 39 36 38 39 36 31 36 39 38 38 39 38 34 31 33 23 35 36 36 + 36 34 36 33 36 36 35 23 26 30 28 30 30 35 33 34 35 33 34 34 34 33 35 33 33 + 27 33 33 33 34 36 23 36 18 28 18 28 30] +>L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 37 39 39 39 39 38 38 + 39 37 39 39 39 39 39 39 39 39 39 39 39 39 38 39 38 39 36 39 39 36 39 35 29 + 34 36 34 38 38 39 39 31 38 38 34 37 38 38 36 38 39 38 33 31 33 33 33 36 36 + 36 36 39 31 33 33 34 34 33 31 33 29 34 36 27 34 31 33 33 36 33 33 32 35 36 + 32 32 29 28 32 31 31 28 25 28 28 30] +>L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 +[36 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 35 38 38 38 38 38 38 38 38 38 38 37 38 37 38 38 38 38 35 38 37 38 38 37 + 29 36 38 36 38 36 37 36 35 23 35 38 35 36 36 36 33 38 35 38 33 35 37 35 33 + 35 33 35 36 36 27 33 36 35 31 31 32 31 23 33 26 28 24 29 23 29 10 29 26 26 + 19 24 26 30] +>L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 39 40 35 40 40 40 40 40 35 40 40 40 40 40 40 40 40 40 40 38 + 35 40 40 40 36 40 40 40 40 40 40 40 40 37 37 37 40 40 40 39 40 40 40 39 36 + 40 40 40 40 38 37 40 40 40 38 40 40 39 40 36 39 36 40 37 35 40 37 40 38 38 + 38 39 36 40 36 35 39 34 38 39 38 39 39 35 36 36 40 36 36 36 36 36 36 38 38 + 38 29 38 35 36 35 33 33 29 32 35 32 35 31 32 26 29 19 27 28 27 24 21 22 25 + 27 27 28 25 30] +>L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 + 39 38 39 39 39 39 39 39 39 39 36 39 39 38 38 33 38 38 38 39 39 39 39 39 39 + 39 39 39 39 38 39 39 39 39 39 39 38 36 38 37 37 38 37 37 38 37 35 37 36 37 + 39 37 35 39 36 36 39 33 34 17 33 36 34 23 28 30 30 32 33 33 33 31 35 31 31 + 29 33 33 33 33 31 20 31 22 22 28 28 16 34 34 31 33 33] +>L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[38 38 38 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 38 40 40 40 40 40 + 35 40 40 40 38 40 40 40 40 40 40 40 39 40 40 40 38 40 40 38 40 40 39 36 38 + 38 38 34 40 40 37 40 40 40 39 40 40 40 40 39 38 39 40 39 39 40 36 39 39 36 + 35 35 39 37 33 40 35 37 37 39 39 37 36 38 38 38 39 38 29 36 36 33 36 33 34 + 36 33 34 31 33 33 33 33 28 21 24 28 11 25 16 23 21 25 36 33 36 33 34 26 26 + 31 25 31] +>L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 39 40 40 40 40 40 40 39 + 39 40 40 40 40 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 38 + 39 39 40 40 40 39 39 40 39 40 40 40 39 40 36 36 36 40 39 40 40 38 40 37 34 + 35 40 33 39 39 40 31 39 39 38 36 35 35 29 33 31 35 33 36 33 33 33 36 36 36 + 33 36 37 34 36 38 36 36 33 27 35 33 33 36 36 33 36 33 31 33 33 27 34 28 33 + 22 31 33 31 34 33 31] +>L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 + 35 40 40 40 40 40 40 40 39 40 39 40 40 38 40 40 38 40 40 39 40 40 40 40 40 + 40 39 39 40 33 40 40 40 40 40 40 40 38 40 36 40 39 38 40 40 40 36 39 40 40 + 31 40 38 40 39 37 35 39 40 38 35 40 39 39 37 36 36 40 38 33 33 27 35 35 37 + 37 37 37 36 37 35 36 33 33 35 38 38 33 37 34 36 36 34 35 35 35 31 35 31 37 + 33 33 35 35 26 33 33 32 32 31 35 24 35 25 25 30 18 28 27 21 20 23 20 20 26 + 25 27] +>L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[39 39 39 38 36 38 38 38 37 38 33 38 38 39 39 39 39 39 39 37 34 39 39 39 39 + 39 39 39 39 39 39 39 39 39 39 35 39 36 37 37 37 38 31 36 36 36 37 27 39 37 + 39 39 39 39 35 39 37 23 35 39 33 39 39 39 32 38 39 38 37 37 35 32 35 35 35 + 37 34 33 36 36 35 35 32 34 36 33 36 33 36 33 22 29 36 29 29 8 29 31 36 35 + 34 34 31 33 27 29 25 20 25 21 25 29 21 23 29 15 32 26 24 29 26 25 31 30 27 + 29 30 30 31 33] +>L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 +[38 38 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 + 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 + 39 39 39 39 38 33 39 39 39 39 39 33 39 39 39 39 39 39 39 37 39 38 39 33 39 + 39 37 38 36 38 36 36 33 36 39 39 39 35 35 35 33 31 38 33 28 30 28 30 36 36 + 36 29 36 33 35 18 28 31 32 32 33 35 35 35 29 33 33 34 32 27 32 28 32] +>L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 +[40 40 40 40 40 39 40 40 37 40 40 40 38 38 39 40 40 39 40 35 40 39 40 40 38 + 33 40 40 40 36 40 40 40 39 39 36 40 40 39 36 38 39 39 39 36 39 38 40 35 33 + 36 38 33 40 37 36 37 37 36 36 39 36 33 39 36 37 34 39 38 38 39 36 39 36 31 + 33 39 33 37 35 37 36 36 38 36 35 35 36 33 33 33 39 36 31 35 33 33 35 33 36 + 33 33 36 35 36 36 35 33 36 29 32 36 34 35 34 35 33 31 33 33 31 25 34 31 33 + 33 31 33 28 15 28 15 29 30 28 29 31] +>L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 + 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 39 40 37 40 40 40 40 40 39 39 + 40 37 40 40 40 39 38 39 39 40 39 40 40 40 39 39 40 40 37 37 40 34 40 33 23 + 33 35 38 35 31 35 33 32 34 37 36 35 38 34 36 36 33 37 37 37 34 33 33 33 36 + 33 36 33 32 32 26 23 30 29 30 32 32 32 33 33 34 34 31 33 33 29 29 34 31 35 + 33 31 33 31 22 31 31 31 23 18 26 23 31 23 24 27 22 10 28 23 28 7 28 20 30 + 24 30] +>L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[27 31 31 38 38 38 38 38 38 38 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 + 38 38 38 38 38 38 35 36 38 38 38 38 38 38 38 38 38 38 38 33 38 38 38 38 38 + 38 35 38 38 38 33 27 33 38 38 38 38 38 38 38 38 38 38 29 27 38 38 38 35 38 + 35 38 38 38 36 38 35 38 38 38 38 38 36 38 37 36 27 35 38 35 35 35 35 38 38 + 38 33 38 35 35 38 35 25 33 33 35 35 35 30 30 25 27 30 38 38 35 33 33 26 33] +>L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 38 40 40 40 40 38 39 40 40 38 38 40 40 40 40 40 39 40 40 40 40 39 + 40 39 40 40 40 39 40 40 40 40 36 40 36 40 37 37 38 38 38 40 37 40 39 40 40 + 39 37 40 39 40 39 35 37 40 40 40 38 36 39 36 39 39 40 39 40 38 38 37 36 38 + 38 27 39 36 38 33 35 29 31 29 32 35 32 36 36 36 31 35 30 32 28 27 28 31 33 + 31 31 31 33 31 33 29 33 33 33 33 34 32 31 31 8 29 31 27 19 29 16 29 31 31 + 29 26 29 32 19 25 31 32] +>L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 + 35 40 40 40 40 40 40 40 40 40 39 40 40 39 40 40 40 40 40 39 40 40 40 39 35 + 40 37 40 40 40 40 40 40 40 40 40 40 39 40 38 40 38 40 39 39 40 36 40 37 37 + 36 40 36 40 39 40 37 40 38 38 39 38 39 35 39 39 39 38 39 38 33 27 36 36 35 + 39 36 37 38 38 39 33 38 36 35 39 39 39 33 34 33 36 33 36 36 35 31 33 23 31 + 31 31 32 31 33 31 31 31 23 30 18 30] +>L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 +[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 39 + 38 40 40 40 39 40 40 40 40 40 40 40 40 40 39 39 40 40 40 40 40 38 37 40 40 + 40 40 40 40 40 40 40 40 40 40 40 39 38 40 37 37 38 40 37 40 40 37 40 38 39 + 33 40 39 38 40 39 40 38 39 38 38 36 35 35 33 35 33 37 35 29 33 31 31 33 33 + 33 28 33 33 35 33 30 31 33 25 33 33 33 36 36 36 34 33 30 30 32 19 31 19 30 + 28 28 30] +>L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 +[28 33 31 38 35 38 38 38 36 38 36 38 38 38 38 38 38 38 38 36 38 38 38 38 38 + 35 38 38 38 38 36 36 36 38 38 38 33 38 38 38 38 38 38 38 35 38 38 38 38 38 + 38 38 31 36 36 33 33 36 30 30 30 35 33 38 38 38 36 38 33 38 38 35 38 38 38 + 38 33 34 30 15 30 27 33 37 38 37 29 33 35 38 35 35 38 34 33 35 35 35 35 35 + 35 35 35 36 38 38 37 35 35 35 32 38 10 7 17 25 18 24 35 33 32 32 32] +>L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 +[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 37 + 37 40 40 40 37 40 40 40 40 40 39 40 40 40 39 36 40 40 40 39 40 40 40 39 39 + 40 37 40 40 40 40 39 40 40 40 39 39 39 40 38 39 39 37 39 40 39 36 40 35 33 + 33 37 38 39 37 39 37 36 39 36 40 40 36 33 35 33 36 36 35 34 35 36 37 28 35 + 33 35 35 34 33 35 28 35 31 33 35 36 33 35 37 34 34 29 33 31 33 19 33 28 31 + 28 29 31 28 33 33 29 23 30] diff -r 000000000000 -r c1bd0c560018 test-data/summarize_taxa_L2.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L2.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 diff -r 000000000000 -r c1bd0c560018 test-data/summarize_taxa_L3.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L3.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125 diff -r 000000000000 -r c1bd0c560018 test-data/summarize_taxa_L4.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L4.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 0.625 0.375 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 0.875 0.125 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.285714285714 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.777777777778 0.222222222222 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 0.75 0.25 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 1.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125 0.875 0.125 diff -r 000000000000 -r c1bd0c560018 test-data/summarize_taxa_L5.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L5.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 0.625 0.375 0.625 0.0 0.0 0.125 0.25 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 0.875 0.125 0.875 0.0 0.0 0.125 0.0 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.0 0.285714285714 0.0 0.0 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.555555555556 0.222222222222 0.0 0.222222222222 0.0 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 0.75 0.25 0.75 0.0 0.0 0.25 0.0 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.0 0.0 0.0 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 1.0 0.0 0.833333333333 0.166666666667 0.0 0.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125 0.875 0.125 0.625 0.25 0.0 0.0 0.125 diff -r 000000000000 -r c1bd0c560018 test-data/summarize_taxa_L6.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/summarize_taxa_L6.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,9 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description k__Bacteria;p__Bacteroidetes k__Bacteria;p__Firmicutes k__Bacteria;p__Bacteroidetes;c__Bacteroidia k__Bacteria;p__Firmicutes;c__Clostridia k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__ k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;Other k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Ruminococcaceae;g__Faecalibacterium +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 0.625 0.375 0.625 0.375 0.625 0.375 0.625 0.0 0.0 0.125 0.25 0.625 0.0 0.0 0.125 0.0 0.25 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 0.875 0.125 0.875 0.125 0.875 0.125 0.875 0.0 0.0 0.125 0.0 0.875 0.0 0.0 0.125 0.0 0.0 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.285714285714 0.714285714286 0.0 0.285714285714 0.0 0.0 0.714285714286 0.0 0.285714285714 0.0 0.0 0.0 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.777777777778 0.222222222222 0.555555555556 0.222222222222 0.0 0.222222222222 0.0 0.555555555556 0.222222222222 0.0 0.0 0.222222222222 0.0 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 0.75 0.25 0.75 0.25 0.75 0.25 0.75 0.0 0.0 0.25 0.0 0.75 0.0 0.0 0.25 0.0 0.0 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.142857142857 0.857142857143 0.0 0.0 0.0 0.142857142857 0.857142857143 0.0 0.0 0.0 0.0 0.142857142857 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 1.0 0.0 1.0 0.0 1.0 0.0 0.833333333333 0.166666666667 0.0 0.0 0.0 0.833333333333 0.166666666667 0.0 0.0 0.0 0.0 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 0.875 0.125 0.875 0.125 0.875 0.125 0.625 0.25 0.0 0.0 0.125 0.625 0.25 0.0 0.0 0.0 0.125 diff -r 000000000000 -r c1bd0c560018 test-data/validate_mapping_file_corrected_map.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/validate_mapping_file_corrected_map.tabular Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,35 @@ +#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description +L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 +L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 +L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 +L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 +L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 +L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 +L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 +L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 +L2S240 CATATCGCAGTT GTGCCAGCMGCCGCGGTAA left palm 2008 10 28 2 Yes 0 2_L_Palm_10_28_2008 +L2S155 ACGATGCGACCA GTGCCAGCMGCCGCGGTAA left palm 2009 1 20 1 No 84 1_L_Palm_1_20_2009 +L2S309 CGTGCATTATCA GTGCCAGCMGCCGCGGTAA left palm 2009 1 20 2 No 84 2_L_Palm_1_20_2009 +L2S175 AGCTATCCACGA GTGCCAGCMGCCGCGGTAA left palm 2009 2 17 1 No 112 1_L_Palm_2_17_2009 +L2S204 ATGCAGCTCAGT GTGCCAGCMGCCGCGGTAA left palm 2009 3 17 1 No 140 1_L_Palm_3_17_2009 +L2S357 CTAACGCAGTCA GTGCCAGCMGCCGCGGTAA left palm 2009 3 17 2 No 140 2_L_Palm_3_17_2009 +L2S222 CACGTGACATGT GTGCCAGCMGCCGCGGTAA left palm 2009 4 14 1 No 168 1_L_Palm_4_14_2009 +L2S382 CTCAATGACTCA GTGCCAGCMGCCGCGGTAA left palm 2009 4 14 2 No 168 2_L_Palm_4_14_2009 +L3S242 ACAGTTGCGCGA GTGCCAGCMGCCGCGGTAA right palm 2008 10 28 1 Yes 0 1_R_Palm_10_28_2008 +L3S378 ATCGATCTGTGG GTGCCAGCMGCCGCGGTAA right palm 2008 10 28 2 Yes 0 2_R_Palm_10_28_2008 +L3S294 CACGACAGGCTA GTGCCAGCMGCCGCGGTAA right palm 2009 1 20 1 No 84 1_R_Palm_1_20_2009 +L4S63 CTCGTGGAGTAG GTGCCAGCMGCCGCGGTAA right palm 2009 1 20 2 No 84 2_R_Palm_1_20_2009 +L3S313 AGTGTCACGGTG GTGCCAGCMGCCGCGGTAA right palm 2009 2 17 1 No 112 1_R_Palm_2_17_2009 +L3S341 CAAGTGAGAGAG GTGCCAGCMGCCGCGGTAA right palm 2009 3 17 1 No 140 1_R_Palm_3_17_2009 +L4S112 GCGTTACACACA GTGCCAGCMGCCGCGGTAA right palm 2009 3 17 2 No 140 2_R_Palm_3_17_2009 +L3S360 CATCGTATCAAC GTGCCAGCMGCCGCGGTAA right palm 2009 4 14 1 No 168 1_R_Palm_4_14_2009 +L4S137 GAACTGTATCTC GTGCCAGCMGCCGCGGTAA right palm 2009 4 14 2 No 168 2_R_Palm_4_14_2009 +L5S104 CAGTGTCAGGAC GTGCCAGCMGCCGCGGTAA tongue 2008 10 28 1 Yes 0 1_Tong_10_28_2008 +L5S240 CTGGACTCATAG GTGCCAGCMGCCGCGGTAA tongue 2008 10 28 2 Yes 0 2_Tong_10_28_2008 +L5S155 ATCTTAGACTGC GTGCCAGCMGCCGCGGTAA tongue 2009 1 20 1 No 84 1_Tong_1_20_2009 +L6S20 GAGGCTCATCAT GTGCCAGCMGCCGCGGTAA tongue 2009 1 20 2 No 84 2_Tong_1_20_2009 +L5S174 CAGACATTGCGT GTGCCAGCMGCCGCGGTAA tongue 2009 2 17 1 No 112 1_Tong_2_17_2009 +L5S203 CGATGCACCAGA GTGCCAGCMGCCGCGGTAA tongue 2009 3 17 1 No 140 1_Tong_3_17_2009 +L6S68 GATACGTCCTGA GTGCCAGCMGCCGCGGTAA tongue 2009 3 17 2 No 140 2_Tong_3_17_2009 +L5S222 CTAGAGACTCTT GTGCCAGCMGCCGCGGTAA tongue 2009 4 14 1 No 168 1_Tong_4_14_2009 +L6S93 GATTAGCACTCT GTGCCAGCMGCCGCGGTAA tongue 2009 4 14 2 No 168 2_Tong_4_14_2009 diff -r 000000000000 -r c1bd0c560018 test-data/validate_mapping_file_log.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/validate_mapping_file_log.txt Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,1 @@ +No errors or warnings found in mapping file. \ No newline at end of file diff -r 000000000000 -r c1bd0c560018 tool-data/qiime_reference_db.loc.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/qiime_reference_db.loc.sample Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,32 @@ +# +# +#For each reference database, you need to download the fasta file in qiime path +# +#List of useful db for qiime: http://qiime.org/home_static/dataFiles.html +# +#silva-119-90 SILVA v.119 90% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set90_aligned.fna +#silva-119-90-16S SILVA v.119 90% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set90_aligned_16S_only.fna +#silva-119-90-18S SILVA v.119 90% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set90_aligned_18S_only.fna +#silva-119-94 SILVA v.119 94% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set94_aligned.fna +#silva-119-94-16S SILVA v.119 94% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set94_aligned_16S_only.fna +#silva-119-94-18S SILVA v.119 94% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set94_aligned_18S_only.fna +#silva-119-97 SILVA v.119 97% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set97_aligned.fna +#silva-119-97-16S SILVA v.119 97% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set97_aligned_16S_only.fna +#silva-119-97-18S SILVA v.119 97% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set97_aligned_18S_only.fna +#silva-119-99 SILVA v.119 99% $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set99_aligned.fna +#silva-119-99-16S SILVA v.119 99% (16S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set99_aligned_16S_only.fna +#silva-119-99-18S SILVA v.119 99% (18S only) $QIIME_ROOT_PATH/reference_db/Silva_119_rep_set99_aligned_18S_only.fna +#gg-13-8-61 Greengenes v.13.8 61% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_61.fasta +#gg-13-8-64 Greengenes v.13.8 64% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_64.fasta +#gg-13-8-67 Greengenes v.13.8 67% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_67.fasta +#gg-13-8-70 Greengenes v.13.8 70% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_70.fasta +#gg-13-8-73 Greengenes v.13.8 73% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_73.fasta +#gg-13-8-76 Greengenes v.13.8 76% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_76.fasta +#gg-13-8-79 Greengenes v.13.8 79% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_79.fasta +#gg-13-8-82 Greengenes v.13.8 82% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_82.fasta +#gg-13-8-85 Greengenes v.13.8 85% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_85.fasta +#gg-13-8-88 Greengenes v.13.8 88% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_88.fasta +#gg-13-8-91 Greengenes v.13.8 91% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_91.fasta +#gg-13-8-94 Greengenes v.13.8 94% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_94.fasta +#gg-13-8-97 Greengenes v.13.8 97% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_97.fasta +#gg-13-8-99 Greengenes v.13.8 99% $QIIME_ROOT_PATH/reference_db/gg_13_8_rep_99.fasta \ No newline at end of file diff -r 000000000000 -r c1bd0c560018 tool_data_table_conf.xml.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,7 @@ + + + + value, name, path + +
+ \ No newline at end of file diff -r 000000000000 -r c1bd0c560018 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,6 @@ + + + + + + diff -r 000000000000 -r c1bd0c560018 upgma_cluster.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/upgma_cluster.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,43 @@ + + Build a UPGMA tree comparing samples + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r c1bd0c560018 validate_mapping_file.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/validate_mapping_file.xml Tue Feb 02 05:50:37 2016 -0500 @@ -0,0 +1,145 @@ + + to check for required data and format + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + suppress_html is False + + + + + + + + + + + + + + + + + + + + + `_. + ]]> + + + + + + +