annotate assign_taxonomy.xml @ 0:c1bd0c560018 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime commit bcbe76277f3e60303faf826f8ce7f018bc663a9a-dirty
author bebatut
date Tue, 02 Feb 2016 05:50:37 -0500
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1 <tool id="qiime_assign_taxonomy" name="assign taxonomy" version="1.9.1galaxy1">
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2
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3 <description>Assign taxonomy to each sequence</description>
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4
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5 <macros>
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6 <import>macros.xml</import>
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7 </macros>
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8
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9 <expand macro="requirements" />
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10
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11 <command>
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12 <![CDATA[
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13 assign_taxonomy.py -i $input_fasta_fp
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14
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15 #if str($id_to_taxonomy_fp) != 'None':
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16 -t $id_to_taxonomy_fp
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17 #end if
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18
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19 #if str($reference_seqs_fp) != 'None':
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20 -r $reference_seqs_fp
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21 #end if
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22
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23 #if str($methodcond.assignment_method) = 'None':
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24 -m uclust
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25 #end if
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26
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27 #if str($methodcond.assignment_method) != 'None':
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28 -m $methodcond.assignment_method
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29 #end if
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30
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31 #if $methodcond.assignment_method == "rtax":
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32
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33 #if $methodcond.single_ok:
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34 --single_ok
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35 #end if
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36
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37 #if $methodcond.no_single_ok_generic:
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38 --no_single_ok_generic
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39 #end if
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40
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41 #if str($methodcond.read_id_regex):
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42 --read_id_regex=$methodcond.read_id_regex
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43 #end if
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44
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45 #if str($methodcond.amplicon_id_regex):
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46 --amplicon_id_regex=$methodcond.amplicon_id_regex
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47 #end if
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48
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49 #if str($methodcond.header_id_regex):
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50 --header_id_regex=$methodcond.header_id_regex
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51 #end if
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52 #end if
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53
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54 #if $methodcond.assignment_method == "sortmerna":
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55
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56 #if str($methodcond.sortmerna_db):
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57 --sortmerna_db=$methodcond.sortmerna_db
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58 #end if
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59
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60 #if $methodcond.sortmerna_e_value:
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61 --sortmerna_e_value=$methodcond.sortmerna_e_value
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62 #end if
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63
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64 #if $methodcond.sortmerna_coverage:
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65 --sortmerna_coverage=$methodcond.sortmerna_coverage
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66 #end if
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67
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68 #if $methodcond.sortmerna_best_N_alignments:
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69 --sortmerna_best_N_alignments=$methodcond.sortmerna_best_N_alignments
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70 #end if
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71
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72 #if str($methodcond.sortmerna_threads):
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73 --sortmerna_threads=$methodcond.sortmerna_threads
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74 #end if
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75
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76 #if $methodcond.min_consensus_fraction:
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77 --min_consensus_fraction=$methodcond.min_consensus_fraction
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78 #end if
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79
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80 #if $methodcond.similarity:
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81 --similarity=$methodcond.similarity
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82 #end if
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83 #end if
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84
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85 #if $methodcond.assignment_method == "blast":
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86
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87 #if str($methodcond.blast_db) != 'None':
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88 -b \$BLAST_DB_NAME
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89 #end if
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90
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91 #if $methodcond.blast_e_value:
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92 -e $methodcond.blast_e_value
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93 #end if
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94 #end if
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95
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96 #if $methodcond.assignment_method == "rdp":
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97
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98 #if $methodcond.confidence:
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99 -c $methodcond.confidence
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100 #end if
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101
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102 #if $methodcond.rdp_max_memory:
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103 --rdp_max_memory=$methodcond.rdp_max_memory
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104 #end if
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105 #end if
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106
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107 #if $methodcond.assignment_method == "mothur":
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108
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109 #if $methodcond.confidence:
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110 -c $methodcond.confidence
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111 #end if
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112 #end if
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113
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114 #if $methodcond.assignment_method == "uclust":
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115
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116 #if $methodcond.min_consensus_fraction:
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117 --min_consensus_fraction=$methodcond.min_consensus_fraction
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118 #end if
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119
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120 #if $methodcond.similarity:
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121 --similarity=$methodcond.similarity
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122 #end if
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123
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124 #if $methodcond.uclust_max_accepts:
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125 --uclust_max_accepts=$methodcond.uclust_max_accepts
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126 #end if
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127 #end if
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128 -o assign_taxonomy_output
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129 ]]>
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130 </command>
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131
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132 <inputs>
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133 <param label="-i/--input_fasta_fp: path to the input fasta file"
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134 name="input_fasta_fp" optional="False" type="data"/>
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135 <param default="/home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/taxonomy/99_otu_taxonomy.txt"
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136 label="-t/--id_to_taxonomy_fp: Path to tab-delimited file mapping
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137 sequences to assigned taxonomy. Each assigned taxonomy is provided as
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138 a semicolon-separated list. For assignment with rdp, each assigned
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139 taxonomy must be exactly 6 levels deep. [default: /home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/taxonomy/99_otu_taxonomy.txt]"
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140 name="id_to_taxonomy_fp" optional="True" type="data"/>
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141 <param default="/home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/rep_set/99_otus.fasta"
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142 label="-r/--reference_seqs_fp: Path to reference sequences. For
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143 assignment with blast, these are used to generate a blast database.
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144 For assignment with rdp, they are used as training sequences for the
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145 classifier. [default: /home12/caparmor/bioinfo/softs/sources/Qiime/data/gg_13_8_otus/rep_set/99_otus.fasta]"
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146 name="reference_seqs_fp" optional="True" type="data"/>
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147
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148 <conditional name="methodcond">
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149 <param label="-m/--assignment_method: Taxon assignment method, must be
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150 one of rdp, blast, rtax, mothur, uclust, sortmerna [default: uclust]"
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151 name="assignment_method" optional="False" type="select">
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152 <option selected="True" value="uclust">uclust</option>
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153 <option value="rdp">rdp</option>
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154 <option value="blast">blast</option>
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155 <option value="rtax">rtax</option>
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156 <option value="mothur">mothur</option>
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157 <option value="sortmerna">sortmerna</option>
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158 </param>
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159 <when value="rtax">
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160 <param label="--single_ok: When classifying paired ends, allow
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161 fallback to single-ended classification when the mate pair is
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162 lacking (used for RTAX only). [default: False]" name="single_ok"
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163 selected="False" type="boolean"/>
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164 <param label="--no_single_ok_generic: When classifying paired ends,
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165 do not allow fallback to single-ended classification when the
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166 mate pair is overly generic (used for RTAX only). [default: False]"
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167 name="no_single_ok_generic" selected="False" type="boolean"/>
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168 <param default="\S+\s+(\S+)" label="--read_id_regex: Used to parse
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169 the result of OTU clustering, to get the read_1_id for each
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170 clusterID. The clusterID itself is assumed to be the first
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171 field, and is not captured by the regex. (used for RTAX only).
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172 [default: \S+\s+(\S+)]" name="read_id_regex" optional="True"
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173 type="text"/>
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174 <param default="(\S+)\s+(\S+?)\/" label="--amplicon_id_regex: Used
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175 to parse the result of split_libraries, to get the ampliconID
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176 for each read_1_id. Two groups capture read_1_id and ampliconID,
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177 respectively. (used for RTAX only). [default: (\S+)\s+(\S+?)\/]"
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178 name="amplicon_id_regex" optional="True" type="text"/>
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179 <param default="\S+\s+(\S+?)\/" label="--header_id_regex: Used to
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180 parse the result of split_libraries, to get the portion of the
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181 header that RTAX uses to match mate pairs. The default uses
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182 the amplicon ID, not including /1 or /3, as the primary key
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183 for the query sequences. Typically this regex will be the
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184 same as amplicon_id_regex, except that only the second group
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185 is captured. (used for RTAX only). [default: \S+\s+(\S+?)\/]"
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186 name="header_id_regex" optional="True" type="text"/>
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187 </when>
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188 <when value="sortmerna">
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189 <param label="--sortmerna_db: Pre-existing database to search
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190 against when using sortmerna [default: None]" name="sortmerna_db"
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191 optional="True" type="text"/>
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192 <param default="1.0" label="--sortmerna_e_value: Maximum E-value
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193 when clustering [default = 1.0]" name="sortmerna_e_value"
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194 optional="True" type="float"/>
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195 <param default="0.9" label="--sortmerna_coverage: Mininum percent
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196 query coverage (of an alignment) to consider a hit, expressed
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197 as a fraction between 0 and 1 [default: 0.9]"
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198 name="sortmerna_coverage" optional="True" type="float"/>
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199 <param default="5" label="--sortmerna_best_N_alignments: This option
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200 specifies how many best alignments per read will be written
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201 [default: 5]" name="sortmerna_best_N_alignments" optional="True"
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202 type="integer"/>
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203 <param default="1" label="--sortmerna_threads: Specify number of
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204 threads to be used for sortmerna mapper which utilizes multithreading.
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205 [default: 1]" name="sortmerna_threads" optional="True"
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206 type="text"/>
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207 <param default="0.51" label="--min_consensus_fraction: Minimum
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208 fraction of database hits that must have a specific taxonomic
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209 assignment to assign that taxonomy to a query, only used for
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210 sortmerna and uclust methods [default: 0.51]"
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211 name="min_consensus_fraction" optional="True" type="float"/>
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212 <param default="0.9" label="--similarity: Minimum percent similarity
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213 (expressed as a fraction between 0 and 1) to consider a database
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214 match a hit, only used for sortmerna and uclust methods
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215 [default: 0.9]" name="similarity" optional="True" type="float"/>
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216 </when>
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217 <when value="blast">
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218 <param label="-b/--blast_db: Database to blast against. Must provide
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219 either --blast_db or --reference_seqs_db for assignment with blast
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220 [default: None]" name="blast_db" optional="True" type="data"/>
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221 <param default="0.001" label="-e/--blast_e_value: Maximum e-value
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222 to record an assignment, only used for blast method [default:
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223 0.001]" name="blast_e_value" optional="True" type="float"/>
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224 </when>
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225 <when value="rdp">
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226 <param default="0.5" label="-c/--confidence: Minimum confidence to
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227 record an assignment, only used for rdp and mothur methods
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228 [default: 0.5]" name="confidence" optional="True" type="float"/>
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229 <param default="4000" label="--rdp_max_memory: Maximum memory
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230 allocation, in MB, for Java virtual machine when using the
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231 rdp method. Increase for large training sets [default: 4000]"
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232 name="rdp_max_memory" optional="True" type="integer"/>
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233 </when>
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234 <when value="mothur">
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235 <param default="0.5" label="-c/--confidence: Minimum confidence to
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236 record an assignment, only used for rdp and mothur methods
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237 [default: 0.5]" name="confidence" optional="True" type="float"/>
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238 </when>
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239 <when value="uclust">
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240 <param default="0.51" label="--min_consensus_fraction: Minimum
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241 fraction of database hits that must have a specific taxonomic
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242 assignment to assign that taxonomy to a query, only used for
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243 sortmerna and uclust methods [default: 0.51]" name="min_consensus_fraction"
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244 optional="True" type="float"/>
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245 <param default="0.9" label="--similarity: Minimum percent similarity
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246 (expressed as a fraction between 0 and 1) to consider a database
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247 match a hit, only used for sortmerna and uclust methods [default:
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248 0.9]" name="similarity" optional="True" type="float"/>
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249 <param default="3" label="--uclust_max_accepts: Number of database
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250 hits to consider when making an assignment, only used for uclust
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251 method [default: 3]" name="uclust_max_accepts" optional="True"
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252 type="integer"/>
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253 </when>
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254 </conditional>
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255 </inputs>
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256
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257 <outputs>
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258 <data format="txt" from_work_dir="assign_taxonomy_output/*.log"
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259 label="tax_assignements.log" name="tax_assignements.log"/>
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260 <data format="txt" from_work_dir="assign_taxonomy_output/*.txt"
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261 label="tax_assignements.txt" name="tax_assignements.txt"/>
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262 </outputs>
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263
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264 <tests>
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265 <test>
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266 </test>
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267 </tests>
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268
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269 <help><![CDATA[
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270 **What it does**
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271
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272 Contains code for assigning taxonomy, using several techniques.
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273
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274 Given a set of sequences, %prog attempts to assign the taxonomy of each sequence.
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275 Currently the methods implemented are assignment with BLAST, the RDP classifier,
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276 RTAX, mothur, and uclust. The output of this step is an observation metadata
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277 mapping file of input sequence identifiers (1st column of output file) to taxonomy
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278 (2nd column) and quality score (3rd column). There may be method-specific information
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279 in subsequent columns.
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280
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281 Reference data sets and id-to-taxonomy maps for 16S rRNA sequences can be found in
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282 the Greengenes reference OTU builds. To get the latest build of the Greengenes OTUs
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283 (and other marker gene OTU collections), follow the "Resources" link from http://qiime.org.
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284 After downloading and unzipping you can use the following files as -r and -t, where
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285 <otus_dir> is the name of the new directory after unzipping the reference OTUs tgz
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286 file.
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287
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288 -r <otus_dir>/rep_set/97_otus.fasta
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289 -t <otus_dir></otus_dir>/taxonomy/97_otu_taxonomy.txt
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290
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291 The consensus taxonomy assignment implemented here is the most detailed lineage
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292 description shared by 90% or more of the sequences within the OTU (this level of
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293 agreement can be adjusted by the user). The full lineage information for each
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294 sequence is one of the output files of the analysis. In addition, a conflict file
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295 records cases in which a phylum-level taxonomy assignment disagreement exists
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296 within an OTU (such instances are rare and can reflect sequence misclassification
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297 within the greengenes database).
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298 ]]>
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299 </help>
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300
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301 <citations>
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302 <expand macro="citations" />
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303 </citations>
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304 </tool>