Mercurial > repos > bebatut > cdhit
diff cd_hit_est.xml @ 3:626cd6334dbc draft
planemo upload for repository https://github.com/ASaiM/galaxytools/tree/master/tools/cdhit/ commit ea0424ae045ac797c080aeabab9a8536f7eb2f84-dirty
author | bebatut |
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date | Mon, 25 Apr 2016 12:13:50 -0400 |
parents | aa923171e225 |
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--- a/cd_hit_est.xml Thu Apr 14 09:24:49 2016 -0400 +++ b/cd_hit_est.xml Mon Apr 25 12:13:50 2016 -0400 @@ -15,82 +15,41 @@ -n $wordsize $strand #include source=$common_cdhit_options# - #include source=$runtime_tuning# ]]></command> <inputs> - <param name="fasta_in" type="data" format="fasta" label="EST Sequences to cluster"/> - <param name="similarity" type="float" value="0.9" label="similarity threshold: .75 - 1.0, default is .9"> - <validator type="in_range" message="sequence similarity threshold should be .75 - 1.0" min=".75" max="1.0"/> - </param> - <param name="wordsize" type="integer" value="8" label="word size"> - <help> Suggested word size: - 8,9,10 for thresholds 0.90 ~ 1.0 - 7 for thresholds 0.88 ~ 0.9 - 6 for thresholds 0.85 ~ 0.88 - 5 for thresholds 0.80 ~ 0.85 - 4 for thresholds 0.75 ~ 0.8 - </help> - <validator type="in_range" message="word size should be between 4 and 10" min="4" max="10"/> - </param> - <param name="strand" type="boolean" truevalue="-r 1" falsevalue="" checked="false" label="Compare both strands"/> + <param name="fasta_in" type="data" format="fasta" label="EST Sequences to cluster" help="(-i)"/> + + <param name="similarity" type="float" value="0.9" label="Similarity threshold" min=".75" max="1.0" help="(-c)"/> + + <param name="wordsize" type="integer" value="8" label="Word size" min="4" max="10" help="It is suggested to adjust word size in function of similarity threshold. 8,9 or 10 for threshold in [0.9;1.0] interval, 7 for [0.88;0.9], 6 for [0.85;0.88], 5 for [0.80;0.85], 4 for [0.75;0.8] (-n)"/> + + <param name="strand" type="boolean" truevalue="-r 1" falsevalue="" checked="false" label="Compare both strands?"/> + <expand macro="common_cdhit_options" /> - <expand macro="runtime_tuning" /> </inputs> <outputs> - <data format="txt" name="clusters_out" label="${tool.name} on ${on_string}: clusters" from_work_dir="rep_seq.clstr"/> - <data format="fasta" name="fasta_out" label="${tool.name} on ${on_string}: representatives.fasta" from_work_dir="rep_seq"/> + <data format="txt" name="clusters_out" label="${tool.name} on ${on_string}: Clusters" from_work_dir="rep_seq.clstr"/> + + <data format="fasta" name="fasta_out" label="${tool.name} on ${on_string}: Representative sequences" from_work_dir="rep_seq"/> </outputs> <tests> - <test> - <!-- Expect 3 clusters: 0,1,2 --> - <param name="fasta_in" value="cd_hit_est_in.fa" /> - <param name="similarity" value="0.9"/> - <param name="wordsize" value="8"/> - <param name="strand" value="true"/> - <!-- conditionals in macros --> - <param name="settings" value="no"/> - <param name="tuning" value="default"/> - <output name="clusters_out"> - <assert_contents> - <has_text text=">Cluster 0" /> - <!-- There should not be a Cluster 3 --> - <not_has_text text="Cluster 3" /> - <has_text_matching expression="F12Fcsw_481739" /> - </assert_contents> - </output> - <output name="fasta_out"> - <assert_contents> - <has_text_matching expression="^>[MF]\d\dFcsw_\d*" /> - </assert_contents> - </output> - </test> - <test> - <!-- tighter constraints should yield more clusters --> - <param name="fasta_in" value="cd_hit_est_in.fa" /> - <param name="similarity" value="0.95"/> - <param name="wordsize" value="9"/> - <param name="strand" value="true"/> - <!-- conditionals in macros --> - <param name="settings" value="no"/> - <param name="tuning" value="default"/> - <output name="clusters_out"> - <assert_contents> - <has_text text=">Cluster 4" /> - <has_text_matching expression=">F12Fcsw_481739" /> - </assert_contents> - </output> - <output name="fasta_out"> - <assert_contents> - <has_text_matching expression="^>[MF]\d\dFcsw_\d*" /> - </assert_contents> - </output> - </test> + <test> + <param name="fasta_in" value="cd_hit_est_in.fa" /> + <param name="similarity" value="0.9"/> + <param name="wordsize" value="8"/> + <param name="strand" value="false"/> + <param name="settings" value="no"/> + <param name="tuning" value="default"/> + <output name="clusters_out" file="est_clusters_output.txt"/> + <output name="fasta_out" file="est_fasta_output.fasta"/> + </test> </tests> <help><![CDATA[ + **What it does** CD-HIT_ stands for Cluster Database at High Identity with Tolerance. The program (cd-hit) takes a fasta format sequence database as input and produces a set of 'non-redundant' (nr) representative sequences as output. In addition cd-hit outputs a cluster file, documenting the sequence 'groupies' for each nr sequence representative. The idea is to reduce the overall size of the database without removing any sequence information by only removing 'redundant' (or highly similar) sequences. This is why the resulting database is called non-redundant (nr). Essentially, cd-hit produces a set of closely related protein families from a given fasta sequence database. @@ -101,15 +60,15 @@ **Inputs** -cd-hit-est requires a fasta dataset as input. - +cd-hit-est requires a fasta file as input. + ------ **Outputs** -A fasta datasets containing representative sequences. +The first output is a fasta file containing representative sequences. -A text file listing the mapping of sequences to the representative sequences:: +The second output is a text file listing the mapping of sequences to the representative sequences:: >Cluster 0 0 2799aa, >PF04998.6|RPOC2_CHLRE/275-3073... * @@ -124,8 +83,6 @@ 1 2208aa, >PF06317.1|Q6IVU4_JUNIN/1-2208... * 2 2207aa, >PF06317.1|Q6IVU0_MACHU/1-2207... at 73% 3 2208aa, >PF06317.1|RRPO_TACV/1-2208... at 69% - - ]]></help> <citations>