Mercurial > repos > bebatut > cdhit

annotate cd_hit_protein.xml @ 4:ba7e9a012723 draft default tip

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planemo upload for repository https://github.com/ASaiM/galaxytools/tree/master/tools/cdhit/ commit 4a97d14fadd86dda8abd088023c7f9f2dcab2a88
author bebatut
date Fri, 04 May 2018 05:40:24 -0400
parents 626cd6334dbc
children
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1 <tool id="cd_hit_protein" name="CD-HIT PROTEIN" version="1.3">
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2 <description>Cluster a protein dataset into representative sequences</description>
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3 <macros>
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4 <import>cdhit_macros.xml</import>
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5 </macros>
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7 <expand macro="requirements"/>
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9 <command><![CDATA[
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10 cd-hit
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11 -i "$fasta_in"
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12 -o rep_seq
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13 -c $similarity
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14 -n $wordsize
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16 #include source=$common_cdhit_options#
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17 ]]></command>
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19 <inputs>
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20 <param name="fasta_in" type="data" format="fasta" label="Protein Sequences to cluster" help="(-i)"/>
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22 <param name="similarity" type="float" value="0.9" label="Similarity threshold" min=".4" max="1.0" help="Similarity threshold must be in [0.4;1.0] interval (-c)"/>
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24 <param name="wordsize" type="integer" value="5" label="Word size" min="2" max="5" help="It is suggested to adjust word size in function of similarity threshold. 5 for threshold in [0.7;1.0] interval, 4 for [0.6;0.7], 3 for [0.5;0.6], 2 for [0.4;0.5] (-n)"/>
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26 <expand macro="common_cdhit_options" />
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27 </inputs>
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29 <outputs>
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30 <data format="txt" name="clusters_out" label="${tool.name} on ${on_string}: Clusters" from_work_dir="rep_seq.clstr"/>
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31 <data format="fasta" name="fasta_out" label="${tool.name} on ${on_string}: Representatives sequences" from_work_dir="rep_seq"/>
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32 </outputs>
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34 <tests>
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35 <test>
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36 <param name="fasta_in" value="cd_hit_protein_in.fasta" />
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37 <param name="similarity" value="0.9" />
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38 <param name="wordsize" value="5" />
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39 <param name="settings" value="no"/>
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40 <param name="print_alignment" value="false"/>
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41 <param name="cluster_type" value="false"/>
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42 <param name="tuning" value="default"/>
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43 <output name="clusters_out" file="protein_clusters_output.txt"/>
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44 <output name="fasta_out" file="protein_fasta_output.fasta"/>
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45 </test>
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46 </tests>
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48 <help><![CDATA[
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49 **What it does**
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51 CD-HIT_ stands for Cluster Database at High Identity with Tolerance. The program (cd-hit) takes a fasta format sequence database as input and produces a set of 'non-redundant' (nr) representative sequences as output. In addition cd-hit outputs a cluster file, documenting the sequence 'groupies' for each nr sequence representative. The idea is to reduce the overall size of the database without removing any sequence information by only removing 'redundant' (or highly similar) sequences. This is why the resulting database is called non-redundant (nr). Essentially, cd-hit produces a set of closely related protein families from a given fasta sequence database.
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53 .. _CD-HIT: http://www.bioinformatics.org/cd-hit/
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55 ------
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57 **Inputs**
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59 cd-hit requires a protein fasta file as input.
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61 ------
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63 **Outputs**
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65 The first output is a fasta file containing representative sequences.
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67 The second output is a text file listing the mapping of sequences to the representative sequences::
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69 >Cluster 0
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70 0 2799aa, >PF04998.6|RPOC2_CHLRE/275-3073... *
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71 >Cluster 1
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72 0 2214aa, >PF06317.1|Q6Y625_9VIRU/1-2214... at 80%
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73 1 2215aa, >PF06317.1|O09705_9VIRU/1-2215... at 84%
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74 2 2217aa, >PF06317.1|Q6Y630_9VIRU/1-2217... *
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75 3 2216aa, >PF06317.1|Q6GWS6_9VIRU/1-2216... at 84%
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76 4 527aa, >PF06317.1|Q67E14_9VIRU/6-532... at 63%
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77 >Cluster 2
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78 0 2202aa, >PF06317.1|Q6UY61_9VIRU/8-2209... at 60%
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79 1 2208aa, >PF06317.1|Q6IVU4_JUNIN/1-2208... *
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80 2 2207aa, >PF06317.1|Q6IVU0_MACHU/1-2207... at 73%
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81 3 2208aa, >PF06317.1|RRPO_TACV/1-2208... at 69%
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82 ]]></help>
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83
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84 <citations>
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85 <citation type="doi">10.1093/bioinformatics/btl158</citation>
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86 <citation type="doi">10.1093/bioinformatics/bts565</citation>
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87 </citations>
0
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88 </tool>