Mercurial > repos > bcclaywell > argo_navis
annotate pact.xml @ 2:7eaf6f9abd28 draft default tip
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| author | bcclaywell |
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| date | Mon, 12 Oct 2015 17:57:38 -0400 |
| parents | d67268158946 |
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| rev | line source |
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1 <tool id="ARGO_pact_run" name="Run PACT" version="1.0.0"> |
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2 <description>Analyze Argo Navis BEAST run using PACT</description> |
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3 <requirements> |
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4 <requirement type="package" version="0.1">argo_env</requirement> |
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5 <requirement type="package" version="3.1.2">R</requirement> |
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6 </requirements> |
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7 <macros> |
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8 <import>macros.xml</import> |
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9 </macros> |
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10 <command interpreter="bash"> |
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11 pact.sh ${config} |
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12 </command> |
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13 <stdio> |
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14 <expand macro="basic_errors"/> |
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15 </stdio> |
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16 <inputs> |
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17 |
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18 <param name="treefile" type="data" format="nex" label="BEAST treefile" |
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19 help="This should be the 'BEAST treefile' from your most recent run of BEAST. |
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20 If you did a 'Resume' run, you should use the 'Trimmed' treefile."/> |
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21 |
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22 <param name="figures_title" type="text" label="Figures title" value="Argo Navis PACT Results" |
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23 help="This string will be set as the title of the PDF figure file returned by this tool. |
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24 Feel free to customize to help you keep track of your files/figures."/> |
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25 |
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26 <conditional name="tip_selection"> |
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27 <param name="method" type="select" label="Tip selection method" |
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28 help="You may optionally choose to restrict these PACT analyses to a subset of the sequences. |
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29 By default, all tips are included in the analysis."> |
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30 <option value="all" selected="true">All tips (no subsetting)</option> |
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31 <option value="demes">Select By Deme</option> |
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32 <option value="names">Space separated tip names</option> |
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33 <option value="file">Tip name file</option> |
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34 </param> |
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35 <when value="demes"> |
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36 <param name="demes" type="text" value="" label="Demes" |
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37 help="Restrict analyses to the specified deme (space seprated if specifying multiple)."/> |
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38 </when> |
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39 <when value="names"> |
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40 <param name="tip_names" type="text" value="" label="Tip names" |
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41 help="Restrict analyses to the specified tip names (space separated)."> |
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42 <sanitizer> |
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43 <valid initial="string.printable"> |
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44 <remove value="'"/> |
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45 </valid> |
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46 <mapping initial="none"> |
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47 <add source="'" target="__sq__"/> |
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48 </mapping> |
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49 </sanitizer> |
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50 </param> |
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51 </when> |
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52 <when value="file"> |
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53 <param name="file" type="data" format="txt" label="Tip names file" |
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54 help="A file with one tip name per line."/> |
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55 </when> |
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56 </conditional> |
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57 |
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58 <conditional name="time_range"> |
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59 <param name="selector" type="select" label="Time range" |
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60 help="As with the Tip selection, you have the choice of restricting the anlayses to a particular time |
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61 range wihtin the tree."> |
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62 <option value="all" selected="true">Don't restrict time range</option> |
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63 <option value="custom">Custom time range</option> |
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64 </param> |
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65 <when value="all"/> |
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66 <when value="custom"> |
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67 <param name="start" type="float" value="1.0" label="Start time" |
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68 help="The age of the oldest ancestry which should be considered (as a positive number)."/> |
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69 <param name="end" type="float" value="0.0" label="End time" optional="true" |
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70 help="The age of the most recent ancestry which should be considered (as a non-negative number). |
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71 Note: this will fail if you specify a time window which excludes all tips in your study."/> |
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72 </when> |
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73 </conditional> |
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74 |
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75 <conditional name="color"> |
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76 <param name="selector" type="select" label="Color specification" |
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77 help="By default, the colors used in plots are automatically chosen for you. |
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78 However, you can either specify your own semi-automatic color scheme using Colorbrewer, or |
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79 for full control a CSV file mapping demes to colors."> |
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80 <option value="default" selected="true">Default</option> |
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81 <option value="brewer">Color brewer theme</option> |
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82 <option value="file">Color spec file</option> |
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83 </param> |
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84 <when value="default"/> <!--Nothing--> |
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85 <when value="brewer"> |
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86 <param name="brewer" type="text" value="RdBu" label="Deme and optionally date information" |
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87 help="A valid colorbrewer2 color scheme name. See http://colorbrewer2.org/ for details."/> |
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88 </when> |
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89 <when value="file"> |
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90 <param name="file" type="data" format="csv" label="Color spec file" |
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91 help="CSV file with 'deme' and 'color' columns, specifying what color (as RGB) each deme should be associated with. |
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92 Note, the default color scheme is both highly contrast and friendly for most color-blind individuals."/> |
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93 </when> |
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94 </conditional> |
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95 |
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96 <param name="custom_pact_settings" type="data" format="txt" label="Custom PACT spec" optional="true" |
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97 help="Not required. You can specify a custom PACT file if you would like to perform some custom anlayses. |
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98 If you do, results will be place in the 'Full data' folder/archive."/> |
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99 |
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100 </inputs> |
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101 |
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102 <outputs> |
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103 <data name="figures" format="pdf" label="PACT figures"/> |
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104 <data name="full_data" format="txt" label="Full data"> |
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105 <discover_datasets pattern="__designation__" ext="txt" directory="working_dir" visible="true"/> |
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106 </data> |
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107 </outputs> |
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108 |
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109 <configfiles> |
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110 <configfile name="config"> |
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111 |
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112 TREEFILE="${treefile}" |
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113 |
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114 FIGURES_TITLE="${figures_title}" |
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115 |
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116 TIP_SELECTION_METHOD="${tip_selection.method}" |
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117 #if $tip_selection.method == "demes" |
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118 DEMES="${tip_selection.demes}" |
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119 #else if $tip_selection.method == "names" |
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120 TIP_NAMES="${tip_selection.tip_names}" |
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121 #else if $tip_selection.method == "file" |
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122 TIP_FILE="${tip_selection.names}" |
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123 #end if |
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124 |
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125 TIME_RANGE_SELECTOR="${time_range.selector}" |
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126 #if $time_range.selector == "custom" |
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127 TIME_RANGE_START="${time_range.start}" |
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128 #if str($time_range.end) != "None" |
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129 TIME_RANGE_END="${time_range.end}" |
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130 #else |
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131 TIME_RANGE_END="" |
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132 #end if |
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133 #end if |
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134 |
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135 COLOR_SELECTOR="${color.selector}" |
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136 #if $color.selector == "default" |
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137 COLOR_SELECTOR="brewer" |
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138 COLOR_BREWER="RdBu" |
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139 #else if $color.selector == "brewer" |
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140 COLOR_BREWER="${color.brewer}" |
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141 #else |
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142 COLOR_FILE="${color.file}" |
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143 #end if |
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144 |
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145 #if str($custom_pact_settings) != "None" |
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146 CUSTOM_PACT_SETTINGS="${custom_pact_settings}" |
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147 #else |
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148 CUSTOM_PACT_SETTINGS="" |
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149 #end if |
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150 |
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151 |
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152 FIGURES="${figures}" |
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153 |
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154 </configfile> |
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155 </configfiles> |
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156 |
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157 <!-- The contents of the help tag is parsed as reStructuredText. Please see |
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158 help-template.rst for examples of commonly-used sections in other Galaxy |
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159 tools. --> |
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160 <help> |
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161 |
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162 .. class:: infomark |
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163 |
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164 What it does |
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165 ------------ |
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166 |
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167 This runs PACT (Posterior Analysis of Coalescent Trees) for an Argo Navis analysis. |
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168 PACT is a tool for analyzing the tree file output of Bayesian discrete trait ancestral analyses. |
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169 Like BEAST, PACT is very powerful and flexible, but can be challenging to get started with. |
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170 The Argo Navis wrapper automates a core set of PACT features, making it easy to operate for beginners. |
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171 |
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172 |
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173 How PACT works |
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174 -------------- |
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175 |
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176 PACT's power and flexibility is due largely to it's combination of |
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177 |
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178 1. A wide range of statistics |
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179 2. Ability to subset the posterior trees, by both tree tips and time windows |
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180 3. Composability of the above features |
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181 |
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182 This lets you ask pointed question about particular aspects of your evolutionary histories. |
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183 |
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184 For example, imagine we're studying a virus which infects several species, including humans, and that we're interested in finding out how |
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185 this virus got into humans. |
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186 To investigate this, we could point PACT towards the viruses found in humans, and even restrict to the time window around when |
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187 transmission into humans occurred. |
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188 Within the context of this restriction, we could look at the proportions of tree length associated with each population, across all of |
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189 the trees in the posterior sample to get a sense of the most likely vector. |
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190 We could also analyze the number of transmissions into the human deme from each of the other host species, and from this estimate |
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191 migration probabilities. |
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192 Comparing these probabilities could also provide support for a particular deme being the zoonotic vector into humans. |
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193 |
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194 |
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195 What Argo Navis' PACT wrapper produces |
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196 -------------------------------------- |
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197 |
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198 Again, while PACT is a very powerful and versatile tool, for the sake of making it easier to use, we focus on a limited set of functionality. |
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199 Most of the information in summarized in a single PDF document with several plots and figures, and a table of statistics. |
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200 |
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201 In particular, the PDF document will contain the following figures: |
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202 |
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203 1. **Maximum likelihood ancestral state tree**: This is the tree from the posterior with the highest likelihood. |
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204 It is colored by deme, and shows one possible history of how the discrete trait may have evolved along with the molecular sequences. |
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205 Keep in mind though, this is only the *most likely* evolutionary course of the molecules and demes under study. |
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206 It's possible the rest of the posterior sample looks quite different, and this should be taken into account when analyzing results. |
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207 This tree is provided as a way of building some intuition about the data, and providing threads you can explore more formally with other tools. |
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208 2. **Skyline proportions**: In the section above about *How PACT works*, we described how you could look at the population proportions for a |
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209 particular portion of the tree are distributed. |
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210 We can take this one step further by using the ``skyline`` functionality of PACT, which enables us to compute a statistic for many different |
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211 small time windows at once. |
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212 By combining these windows, we get a picture of how the proportions are shifting over time. |
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213 3. **Migration rates**: As mentioned above, migration rates can be estimated based on the posterior trees. |
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214 This is graphically represented via a table of boxplots, representing the mean and 95% confidence intervals of the migration rates to and |
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215 from each population. |
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216 The rows of this table correspond to migration "origin", and columns to "destination". |
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217 Additionally, the boxes are colored by migration origin, with the same scheme as used for other plots. |
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218 4. **Additional statistics**: Additional statistics are presented in textual table format. |
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219 |
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220 |
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221 Argo Navis PACT controls |
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222 ------------------------ |
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223 |
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224 All you need in order to run the PACT tool is the treefile output from your BEAST run. |
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225 Note that if you have done a *resume* run, you should use the **trimmed** version of the treefile, so PACT doesn't get too bogged down |
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226 with a large data set. |
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227 In addition to the data though, you can specify: |
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228 |
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229 1. **Figure title**: A title to display at the top of your PDF document. |
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230 2. **Tip selection**: Restrict analyses to a particular set of tree tips. |
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231 You're choices here include: |
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232 a) leaving all tree tips in the analysis |
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233 b) subsetting to just the tree tips corresponding to a specific deme |
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234 c) subsetting to an arbitrary collection of tree tips via text field |
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235 d) subsetting to an arbitrary collection of tree tips, as specified by an input file |
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236 3. **Temporal select**: Restrict analyses to a particular time window. |
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237 You can specify either or both of start time and stop time. |
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238 By default, analyses consider the entire history of the posterior trees, and the skyline proportions go as far back as the mean TMRCA. |
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239 4. **Color specification**: You can customize the color scheme used in the graphics, either using a `ColorBrewer`_ pallete, or a CSV file |
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240 mapping demes to RGB color specifications |
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241 |
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242 If you would like to run more customized analyses, you can also specify a custom PACT config file. |
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243 However, this tool does not support the PDF output for more customized analyses; |
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244 You will instead get individual textual output files, which you will have to process/interpret as you see fit. |
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245 For guidance in this, we recommend you take a look at the `PACT documentation`_. |
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246 |
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247 |
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248 .. _PACT documentation: https://github.com/trvrb/PACT/blob/master/pact_manual.pdf?raw=true |
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249 .. _ColorBrewer: http://colorbrewer2.org |
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250 |
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251 |
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252 </help> |
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253 </tool> |
