annotate pact.xml @ 2:7eaf6f9abd28 draft default tip

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date Mon, 12 Oct 2015 17:57:38 -0400
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1 <tool id="ARGO_pact_run" name="Run PACT" version="1.0.0">
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2 <description>Analyze Argo Navis BEAST run using PACT</description>
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3 <requirements>
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4 <requirement type="package" version="0.1">argo_env</requirement>
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5 <requirement type="package" version="3.1.2">R</requirement>
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6 </requirements>
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7 <macros>
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8 <import>macros.xml</import>
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9 </macros>
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10 <command interpreter="bash">
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11 pact.sh ${config}
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12 </command>
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13 <stdio>
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14 <expand macro="basic_errors"/>
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15 </stdio>
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16 <inputs>
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17
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18 <param name="treefile" type="data" format="nex" label="BEAST treefile"
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19 help="This should be the 'BEAST treefile' from your most recent run of BEAST.
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20 If you did a 'Resume' run, you should use the 'Trimmed' treefile."/>
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21
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22 <param name="figures_title" type="text" label="Figures title" value="Argo Navis PACT Results"
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23 help="This string will be set as the title of the PDF figure file returned by this tool.
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24 Feel free to customize to help you keep track of your files/figures."/>
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25
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26 <conditional name="tip_selection">
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27 <param name="method" type="select" label="Tip selection method"
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28 help="You may optionally choose to restrict these PACT analyses to a subset of the sequences.
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29 By default, all tips are included in the analysis.">
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30 <option value="all" selected="true">All tips (no subsetting)</option>
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31 <option value="demes">Select By Deme</option>
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32 <option value="names">Space separated tip names</option>
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33 <option value="file">Tip name file</option>
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34 </param>
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35 <when value="demes">
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36 <param name="demes" type="text" value="" label="Demes"
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37 help="Restrict analyses to the specified deme (space seprated if specifying multiple)."/>
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38 </when>
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39 <when value="names">
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40 <param name="tip_names" type="text" value="" label="Tip names"
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41 help="Restrict analyses to the specified tip names (space separated).">
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42 <sanitizer>
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43 <valid initial="string.printable">
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44 <remove value="&apos;"/>
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45 </valid>
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46 <mapping initial="none">
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47 <add source="&apos;" target="__sq__"/>
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48 </mapping>
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49 </sanitizer>
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50 </param>
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51 </when>
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52 <when value="file">
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53 <param name="file" type="data" format="txt" label="Tip names file"
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54 help="A file with one tip name per line."/>
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55 </when>
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56 </conditional>
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57
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58 <conditional name="time_range">
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59 <param name="selector" type="select" label="Time range"
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60 help="As with the Tip selection, you have the choice of restricting the anlayses to a particular time
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61 range wihtin the tree.">
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62 <option value="all" selected="true">Don't restrict time range</option>
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63 <option value="custom">Custom time range</option>
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64 </param>
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65 <when value="all"/>
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66 <when value="custom">
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67 <param name="start" type="float" value="1.0" label="Start time"
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68 help="The age of the oldest ancestry which should be considered (as a positive number)."/>
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69 <param name="end" type="float" value="0.0" label="End time" optional="true"
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70 help="The age of the most recent ancestry which should be considered (as a non-negative number).
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71 Note: this will fail if you specify a time window which excludes all tips in your study."/>
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72 </when>
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73 </conditional>
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74
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75 <conditional name="color">
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76 <param name="selector" type="select" label="Color specification"
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77 help="By default, the colors used in plots are automatically chosen for you.
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78 However, you can either specify your own semi-automatic color scheme using Colorbrewer, or
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79 for full control a CSV file mapping demes to colors.">
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80 <option value="default" selected="true">Default</option>
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81 <option value="brewer">Color brewer theme</option>
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82 <option value="file">Color spec file</option>
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83 </param>
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84 <when value="default"/> <!--Nothing-->
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85 <when value="brewer">
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86 <param name="brewer" type="text" value="RdBu" label="Deme and optionally date information"
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87 help="A valid colorbrewer2 color scheme name. See http://colorbrewer2.org/ for details."/>
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88 </when>
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89 <when value="file">
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90 <param name="file" type="data" format="csv" label="Color spec file"
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91 help="CSV file with 'deme' and 'color' columns, specifying what color (as RGB) each deme should be associated with.
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92 Note, the default color scheme is both highly contrast and friendly for most color-blind individuals."/>
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93 </when>
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94 </conditional>
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95
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96 <param name="custom_pact_settings" type="data" format="txt" label="Custom PACT spec" optional="true"
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97 help="Not required. You can specify a custom PACT file if you would like to perform some custom anlayses.
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98 If you do, results will be place in the 'Full data' folder/archive."/>
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99
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100 </inputs>
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101
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102 <outputs>
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103 <data name="figures" format="pdf" label="PACT figures"/>
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104 <data name="full_data" format="txt" label="Full data">
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105 <discover_datasets pattern="__designation__" ext="txt" directory="working_dir" visible="true"/>
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106 </data>
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107 </outputs>
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108
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109 <configfiles>
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110 <configfile name="config">
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111
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112 TREEFILE="${treefile}"
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113
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114 FIGURES_TITLE="${figures_title}"
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115
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116 TIP_SELECTION_METHOD="${tip_selection.method}"
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117 #if $tip_selection.method == "demes"
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118 DEMES="${tip_selection.demes}"
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119 #else if $tip_selection.method == "names"
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120 TIP_NAMES="${tip_selection.tip_names}"
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121 #else if $tip_selection.method == "file"
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122 TIP_FILE="${tip_selection.names}"
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123 #end if
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124
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125 TIME_RANGE_SELECTOR="${time_range.selector}"
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126 #if $time_range.selector == "custom"
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127 TIME_RANGE_START="${time_range.start}"
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128 #if str($time_range.end) != "None"
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129 TIME_RANGE_END="${time_range.end}"
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130 #else
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131 TIME_RANGE_END=""
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132 #end if
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133 #end if
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134
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135 COLOR_SELECTOR="${color.selector}"
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136 #if $color.selector == "default"
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137 COLOR_SELECTOR="brewer"
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138 COLOR_BREWER="RdBu"
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139 #else if $color.selector == "brewer"
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140 COLOR_BREWER="${color.brewer}"
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141 #else
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142 COLOR_FILE="${color.file}"
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143 #end if
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144
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145 #if str($custom_pact_settings) != "None"
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146 CUSTOM_PACT_SETTINGS="${custom_pact_settings}"
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147 #else
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148 CUSTOM_PACT_SETTINGS=""
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149 #end if
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150
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151
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152 FIGURES="${figures}"
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153
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154 </configfile>
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155 </configfiles>
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156
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157 <!-- The contents of the help tag is parsed as reStructuredText. Please see
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158 help-template.rst for examples of commonly-used sections in other Galaxy
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159 tools. -->
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160 <help>
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161
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162 .. class:: infomark
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163
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164 What it does
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165 ------------
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166
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167 This runs PACT (Posterior Analysis of Coalescent Trees) for an Argo Navis analysis.
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168 PACT is a tool for analyzing the tree file output of Bayesian discrete trait ancestral analyses.
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169 Like BEAST, PACT is very powerful and flexible, but can be challenging to get started with.
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170 The Argo Navis wrapper automates a core set of PACT features, making it easy to operate for beginners.
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171
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172
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173 How PACT works
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174 --------------
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175
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176 PACT's power and flexibility is due largely to it's combination of
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177
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178 1. A wide range of statistics
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179 2. Ability to subset the posterior trees, by both tree tips and time windows
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180 3. Composability of the above features
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181
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182 This lets you ask pointed question about particular aspects of your evolutionary histories.
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183
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184 For example, imagine we're studying a virus which infects several species, including humans, and that we're interested in finding out how
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185 this virus got into humans.
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186 To investigate this, we could point PACT towards the viruses found in humans, and even restrict to the time window around when
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187 transmission into humans occurred.
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188 Within the context of this restriction, we could look at the proportions of tree length associated with each population, across all of
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189 the trees in the posterior sample to get a sense of the most likely vector.
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190 We could also analyze the number of transmissions into the human deme from each of the other host species, and from this estimate
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191 migration probabilities.
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192 Comparing these probabilities could also provide support for a particular deme being the zoonotic vector into humans.
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193
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194
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195 What Argo Navis' PACT wrapper produces
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196 --------------------------------------
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197
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198 Again, while PACT is a very powerful and versatile tool, for the sake of making it easier to use, we focus on a limited set of functionality.
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199 Most of the information in summarized in a single PDF document with several plots and figures, and a table of statistics.
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200
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201 In particular, the PDF document will contain the following figures:
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202
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203 1. **Maximum likelihood ancestral state tree**: This is the tree from the posterior with the highest likelihood.
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204 It is colored by deme, and shows one possible history of how the discrete trait may have evolved along with the molecular sequences.
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205 Keep in mind though, this is only the *most likely* evolutionary course of the molecules and demes under study.
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206 It's possible the rest of the posterior sample looks quite different, and this should be taken into account when analyzing results.
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207 This tree is provided as a way of building some intuition about the data, and providing threads you can explore more formally with other tools.
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208 2. **Skyline proportions**: In the section above about *How PACT works*, we described how you could look at the population proportions for a
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209 particular portion of the tree are distributed.
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210 We can take this one step further by using the ``skyline`` functionality of PACT, which enables us to compute a statistic for many different
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211 small time windows at once.
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212 By combining these windows, we get a picture of how the proportions are shifting over time.
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213 3. **Migration rates**: As mentioned above, migration rates can be estimated based on the posterior trees.
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214 This is graphically represented via a table of boxplots, representing the mean and 95% confidence intervals of the migration rates to and
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215 from each population.
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216 The rows of this table correspond to migration "origin", and columns to "destination".
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217 Additionally, the boxes are colored by migration origin, with the same scheme as used for other plots.
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218 4. **Additional statistics**: Additional statistics are presented in textual table format.
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219
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220
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221 Argo Navis PACT controls
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222 ------------------------
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223
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224 All you need in order to run the PACT tool is the treefile output from your BEAST run.
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225 Note that if you have done a *resume* run, you should use the **trimmed** version of the treefile, so PACT doesn't get too bogged down
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226 with a large data set.
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227 In addition to the data though, you can specify:
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228
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229 1. **Figure title**: A title to display at the top of your PDF document.
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230 2. **Tip selection**: Restrict analyses to a particular set of tree tips.
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231 You're choices here include:
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232 a) leaving all tree tips in the analysis
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233 b) subsetting to just the tree tips corresponding to a specific deme
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234 c) subsetting to an arbitrary collection of tree tips via text field
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235 d) subsetting to an arbitrary collection of tree tips, as specified by an input file
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236 3. **Temporal select**: Restrict analyses to a particular time window.
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237 You can specify either or both of start time and stop time.
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238 By default, analyses consider the entire history of the posterior trees, and the skyline proportions go as far back as the mean TMRCA.
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239 4. **Color specification**: You can customize the color scheme used in the graphics, either using a `ColorBrewer`_ pallete, or a CSV file
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240 mapping demes to RGB color specifications
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241
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242 If you would like to run more customized analyses, you can also specify a custom PACT config file.
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243 However, this tool does not support the PDF output for more customized analyses;
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244 You will instead get individual textual output files, which you will have to process/interpret as you see fit.
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245 For guidance in this, we recommend you take a look at the `PACT documentation`_.
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246
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247
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248 .. _PACT documentation: https://github.com/trvrb/PACT/blob/master/pact_manual.pdf?raw=true
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249 .. _ColorBrewer: http://colorbrewer2.org
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250
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251
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252 </help>
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253 </tool>