Mercurial > repos > aaronquinlan > multi_intersect
diff BEDTools-Version-2.14.3/src/windowBed/windowMain.cpp @ 0:dfcd8b6c1bda
Uploaded
author | aaronquinlan |
---|---|
date | Thu, 03 Nov 2011 10:25:04 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/BEDTools-Version-2.14.3/src/windowBed/windowMain.cpp Thu Nov 03 10:25:04 2011 -0400 @@ -0,0 +1,263 @@ +/***************************************************************************** + windowMain.cpp + + (c) 2009 - Aaron Quinlan + Hall Laboratory + Department of Biochemistry and Molecular Genetics + University of Virginia + aaronquinlan@gmail.com + + Licenced under the GNU General Public License 2.0 license. +******************************************************************************/ +#include "windowBed.h" +#include "version.h" + +using namespace std; + +// define the version +#define PROGRAM_NAME "windowBed" + +// define our parameter checking macro +#define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) + +// function declarations +void ShowHelp(void); + + +int main(int argc, char* argv[]) { + + // our configuration variables + bool showHelp = false; + + // input files + string bedAFile; + string bedBFile; + + // input arguments + int leftSlop = 1000; + int rightSlop = 1000; + + bool haveBedA = false; + bool haveBedB = false; + bool noHit = false; + bool anyHit = false; + bool writeCount = false; + bool haveSlop = false; + bool haveLeft = false; + bool haveRight = false; + bool strandWindows = false; + bool matchOnSameStrand = false; + bool matchOnDiffStrand = false; + bool inputIsBam = false; + bool outputIsBam = true; + bool uncompressedBam = false; + + // check to see if we should print out some help + if(argc <= 1) showHelp = true; + + for(int i = 1; i < argc; i++) { + int parameterLength = (int)strlen(argv[i]); + + if((PARAMETER_CHECK("-h", 2, parameterLength)) || + (PARAMETER_CHECK("--help", 5, parameterLength))) { + showHelp = true; + } + } + + if(showHelp) ShowHelp(); + + // do some parsing (all of these parameters require 2 strings) + for(int i = 1; i < argc; i++) { + + int parameterLength = (int)strlen(argv[i]); + + if(PARAMETER_CHECK("-a", 2, parameterLength)) { + if ((i+1) < argc) { + haveBedA = true; + bedAFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-abam", 5, parameterLength)) { + if ((i+1) < argc) { + haveBedA = true; + inputIsBam = true; + bedAFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-b", 2, parameterLength)) { + if ((i+1) < argc) { + haveBedB = true; + bedBFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-bed", 4, parameterLength)) { + outputIsBam = false; + } + else if(PARAMETER_CHECK("-u", 2, parameterLength)) { + anyHit = true; + } + else if(PARAMETER_CHECK("-c", 2, parameterLength)) { + writeCount = true; + } + else if (PARAMETER_CHECK("-v", 2, parameterLength)) { + noHit = true; + } + else if (PARAMETER_CHECK("-sw", 3, parameterLength)) { + strandWindows = true; + } + else if (PARAMETER_CHECK("-sm", 3, parameterLength)) { + matchOnSameStrand = true; + } + else if (PARAMETER_CHECK("-Sm", 3, parameterLength)) { + matchOnDiffStrand = true; + } + else if (PARAMETER_CHECK("-w", 2, parameterLength)) { + if ((i+1) < argc) { + haveSlop = true; + leftSlop = atoi(argv[i + 1]); + rightSlop = leftSlop; + i++; + } + } + else if (PARAMETER_CHECK("-l", 2, parameterLength)) { + if ((i+1) < argc) { + haveLeft = true; + leftSlop = atoi(argv[i + 1]); + i++; + } + } + else if (PARAMETER_CHECK("-r", 2, parameterLength)) { + if ((i+1) < argc) { + haveRight = true; + rightSlop = atoi(argv[i + 1]); + i++; + } + } + else if(PARAMETER_CHECK("-ubam", 5, parameterLength)) { + uncompressedBam = true; + } + else { + cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; + showHelp = true; + } + } + + // make sure we have both input files + if (!haveBedA || !haveBedB) { + cerr << endl << "*****" << endl << "*****ERROR: Need -a and -b files. " << endl << "*****" << endl; + showHelp = true; + } + + if (anyHit && noHit) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -u OR -v, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (anyHit && writeCount) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -u OR -c, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (haveLeft && (leftSlop < 0)) { + cerr << endl << "*****" << endl << "*****ERROR: Upstream window (-l) must be positive." << endl << "*****" << endl; + showHelp = true; + } + + if (haveRight && (rightSlop < 0)) { + cerr << endl << "*****" << endl << "*****ERROR: Downstream window (-r) must be positive." << endl << "*****" << endl; + showHelp = true; + } + + if (haveSlop && (haveLeft || haveRight)) { + cerr << endl << "*****" << endl << "*****ERROR: Cannot choose -w with -l or -r. Either specify -l and -r or specify solely -w" << endl << "*****" << endl; + showHelp = true; + } + + if ((haveLeft && !haveRight) || (haveRight && !haveLeft)) { + cerr << endl << "*****" << endl << "*****ERROR: Please specify both -l and -r." << endl << "*****" << endl; + showHelp = true; + } + + if (matchOnSameStrand && matchOnDiffStrand) { + cerr << endl << "*****" << endl << "*****ERROR: Use either -sm or -Sm, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (!showHelp) { + BedWindow *bi = new BedWindow(bedAFile, bedBFile, leftSlop, rightSlop, anyHit, + noHit, writeCount, strandWindows, matchOnSameStrand, matchOnDiffStrand, + inputIsBam, outputIsBam, uncompressedBam); + delete bi; + return 0; + } + else { + ShowHelp(); + } +} + + +void ShowHelp(void) { + + cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; + + cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; + + cerr << "Summary: Examines a \"window\" around each feature in A and" << endl; + cerr << "\t reports all features in B that overlap the window. For each" << endl; + cerr << "\t overlap the entire entry in A and B are reported." << endl << endl; + + cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -a <bed/gff/vcf> -b <bed/gff/vcf>" << endl << endl; + + cerr << "Options: " << endl; + + cerr << "\t-abam\t" << "The A input file is in BAM format. Output will be BAM as well." << endl << endl; + + cerr << "\t-ubam\t" << "Write uncompressed BAM output. Default is to write compressed BAM." << endl << endl; + + cerr << "\t-bed\t" << "When using BAM input (-abam), write output as BED. The default" << endl; + cerr << "\t\tis to write output in BAM when using -abam." << endl << endl; + + cerr << "\t-w\t" << "Base pairs added upstream and downstream of each entry" << endl; + cerr << "\t\tin A when searching for overlaps in B." << endl; + cerr << "\t\t- Creates symterical \"windows\" around A." << endl; + cerr << "\t\t- Default is 1000 bp." << endl; + cerr << "\t\t- (INTEGER)" << endl << endl; + + cerr << "\t-l\t" << "Base pairs added upstream (left of) of each entry" << endl; + cerr << "\t\tin A when searching for overlaps in B." << endl; + cerr << "\t\t- Allows one to define assymterical \"windows\"." << endl; + cerr << "\t\t- Default is 1000 bp." << endl; + cerr << "\t\t- (INTEGER)" << endl << endl; + + cerr << "\t-r\t" << "Base pairs added downstream (right of) of each entry" << endl; + cerr << "\t\tin A when searching for overlaps in B." << endl; + cerr << "\t\t- Allows one to define assymterical \"windows\"." << endl; + cerr << "\t\t- Default is 1000 bp." << endl; + cerr << "\t\t- (INTEGER)" << endl << endl; + + cerr << "\t-sw\t" << "Define -l and -r based on strand. For example if used, -l 500" << endl; + cerr << "\t\tfor a negative-stranded feature will add 500 bp downstream." << endl; + cerr << "\t\t- Default = disabled." << endl << endl; + + cerr << "\t-sm\t" << "Only report hits in B that overlap A on the _same_ strand." << endl; + cerr << "\t\t- By default, overlaps are reported without respect to strand." << endl << endl; + + cerr << "\t-Sm\t" << "Only report hits in B that overlap A on the _opposite_ strand." << endl; + cerr << "\t\t- By default, overlaps are reported without respect to strand." << endl << endl; + + cerr << "\t-u\t" << "Write the original A entry _once_ if _any_ overlaps found in B." << endl; + cerr << "\t\t- In other words, just report the fact >=1 hit was found." << endl << endl; + + cerr << "\t-c\t" << "For each entry in A, report the number of overlaps with B." << endl; + cerr << "\t\t- Reports 0 for A entries that have no overlap with B." << endl; + cerr << "\t\t- Overlaps restricted by -f." << endl << endl; + + cerr << "\t-v\t" << "Only report those entries in A that have _no overlaps_ with B." << endl; + cerr << "\t\t- Similar to \"grep -v.\"" << endl << endl; + + // end the program here + exit(1); +}