Mercurial > repos > aaronquinlan > multi_intersect
diff BEDTools-Version-2.14.3/src/slopBed/slopBedMain.cpp @ 0:dfcd8b6c1bda
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author | aaronquinlan |
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date | Thu, 03 Nov 2011 10:25:04 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/BEDTools-Version-2.14.3/src/slopBed/slopBedMain.cpp Thu Nov 03 10:25:04 2011 -0400 @@ -0,0 +1,190 @@ +/***************************************************************************** + slopBedMain.cpp + + (c) 2009 - Aaron Quinlan + Hall Laboratory + Department of Biochemistry and Molecular Genetics + University of Virginia + aaronquinlan@gmail.com + + Licenced under the GNU General Public License 2.0 license. +******************************************************************************/ +#include "slopBed.h" +#include "version.h" + +using namespace std; + +// define our program name +#define PROGRAM_NAME "slopBed" + + +// define our parameter checking macro +#define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) + +// function declarations +void ShowHelp(void); + +int main(int argc, char* argv[]) { + + // our configuration variables + bool showHelp = false; + + // input files + string bedFile = "stdin"; + string genomeFile; + + bool haveBed = true; + bool haveGenome = false; + bool haveLeft = false; + bool haveRight = false; + bool haveBoth = false; + + bool forceStrand = false; + float leftSlop = 0.0; + float rightSlop = 0.0; + bool fractional = false; + + for(int i = 1; i < argc; i++) { + int parameterLength = (int)strlen(argv[i]); + + if((PARAMETER_CHECK("-h", 2, parameterLength)) || + (PARAMETER_CHECK("--help", 5, parameterLength))) { + showHelp = true; + } + } + + if(showHelp) ShowHelp(); + + // do some parsing (all of these parameters require 2 strings) + for(int i = 1; i < argc; i++) { + + int parameterLength = (int)strlen(argv[i]); + + if(PARAMETER_CHECK("-i", 2, parameterLength)) { + if ((i+1) < argc) { + bedFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-g", 2, parameterLength)) { + if ((i+1) < argc) { + haveGenome = true; + genomeFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-l", 2, parameterLength)) { + if ((i+1) < argc) { + haveLeft = true; + leftSlop = atof(argv[i + 1]); + i++; + } + } + else if(PARAMETER_CHECK("-r", 2, parameterLength)) { + if ((i+1) < argc) { + haveRight = true; + rightSlop = atof(argv[i + 1]); + i++; + } + } + else if(PARAMETER_CHECK("-b", 2, parameterLength)) { + if ((i+1) < argc) { + haveBoth = true; + leftSlop = atof(argv[i + 1]); + rightSlop = atof(argv[i + 1]); + i++; + } + } + else if(PARAMETER_CHECK("-s", 2, parameterLength)) { + forceStrand = true; + } + else if(PARAMETER_CHECK("-pct", 4, parameterLength)) { + fractional = true; + } + else { + cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; + showHelp = true; + } + } + + // make sure we have both input files + if (!haveBed || !haveGenome) { + cerr << endl << "*****" << endl << "*****ERROR: Need both a BED (-i) and a genome (-g) file. " << endl << "*****" << endl; + showHelp = true; + } + if (!haveLeft && !haveRight && !haveBoth) { + cerr << endl << "*****" << endl << "*****ERROR: Need -l and -r together or -b alone. " << endl << "*****" << endl; + showHelp = true; + } + if ((!haveLeft && haveRight) || (haveLeft && !haveRight)) { + cerr << endl << "*****" << endl << "*****ERROR: Need both -l and -r. " << endl << "*****" << endl; + showHelp = true; + } + if (forceStrand && (!(haveLeft) || !(haveRight))) { + cerr << endl << "*****" << endl << "*****ERROR: Must supply -l and -r with -s. " << endl << "*****" << endl; + showHelp = true; + } + + if (!showHelp) { + BedSlop *bc = new BedSlop(bedFile, genomeFile, forceStrand, leftSlop, rightSlop, fractional); + delete bc; + + return 0; + } + else { + ShowHelp(); + } +} + +void ShowHelp(void) { + + cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; + + cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; + + cerr << "Summary: Add requested base pairs of \"slop\" to each feature." << endl << endl; + + cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -i <bed/gff/vcf> -g <genome> [-b <int> or (-l and -r)]" << endl << endl; + + cerr << "Options: " << endl; + cerr << "\t-b\t" << "Increase the BED/GFF/VCF entry by -b base pairs in each direction." << endl; + cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; + + cerr << "\t-l\t" << "The number of base pairs to subtract from the start coordinate." << endl; + cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; + + cerr << "\t-r\t" << "The number of base pairs to add to the end coordinate." << endl; + cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; + + cerr << "\t-s\t" << "Define -l and -r based on strand." << endl; + cerr << "\t\tE.g. if used, -l 500 for a negative-stranded feature, " << endl; + cerr << "\t\tit will add 500 bp downstream. Default = false." << endl << endl; + + cerr << "\t-pct\t" << "Define -l and -r as a fraction of the feature's length." << endl; + cerr << "\t\tE.g. if used on a 1000bp feature, -l 0.50, " << endl; + cerr << "\t\twill add 500 bp \"upstream\". Default = false." << endl << endl; + + cerr << "Notes: " << endl; + cerr << "\t(1) Starts will be set to 0 if options would force it below 0." << endl; + cerr << "\t(2) Ends will be set to the chromosome length if requested slop would" << endl; + cerr << "\tforce it above the max chrom length." << endl; + + cerr << "\t(3) The genome file should tab delimited and structured as follows:" << endl; + cerr << "\n\t<chromName><TAB><chromSize>" << endl << endl; + cerr << "\tFor example, Human (hg19):" << endl; + cerr << "\tchr1\t249250621" << endl; + cerr << "\tchr2\t243199373" << endl; + cerr << "\t..." << endl; + cerr << "\tchr18_gl000207_random\t4262" << endl << endl; + + + cerr << "Tips: " << endl; + cerr << "\tOne can use the UCSC Genome Browser's MySQL database to extract" << endl; + cerr << "\tchromosome sizes. For example, H. sapiens:" << endl << endl; + cerr << "\tmysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -e \\" << endl; + cerr << "\t\"select chrom, size from hg19.chromInfo\" > hg19.genome" << endl << endl; + + + // end the program here + exit(1); +}