Mercurial > repos > aaronquinlan > multi_intersect
diff BEDTools-Version-2.14.3/src/shuffleBed/shuffleBedMain.cpp @ 0:dfcd8b6c1bda
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author | aaronquinlan |
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date | Thu, 03 Nov 2011 10:25:04 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/BEDTools-Version-2.14.3/src/shuffleBed/shuffleBedMain.cpp Thu Nov 03 10:25:04 2011 -0400 @@ -0,0 +1,188 @@ +/***************************************************************************** + shuffleBedMain.cpp + + (c) 2009 - Aaron Quinlan + Hall Laboratory + Department of Biochemistry and Molecular Genetics + University of Virginia + aaronquinlan@gmail.com + + Licenced under the GNU General Public License 2.0 license. +******************************************************************************/ +#include "shuffleBed.h" +#include "version.h" + +using namespace std; + +// define our program name +#define PROGRAM_NAME "shuffleBed" + + +// define our parameter checking macro +#define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) + +// function declarations +void ShowHelp(void); + +int main(int argc, char* argv[]) { + + // our configuration variables + bool showHelp = false; + + // input files + string bedFile = "stdin"; + string excludeFile; + string includeFile; + string genomeFile; + + bool haveBed = true; + bool haveGenome = false; + bool haveExclude = false; + bool haveInclude = false; + bool haveSeed = false; + float overlapFraction = 0.0; + int seed = -1; + bool sameChrom = false; + + + for(int i = 1; i < argc; i++) { + int parameterLength = (int)strlen(argv[i]); + + if((PARAMETER_CHECK("-h", 2, parameterLength)) || + (PARAMETER_CHECK("--help", 5, parameterLength))) { + showHelp = true; + } + } + + if(showHelp) ShowHelp(); + + // do some parsing (all of these parameters require 2 strings) + for(int i = 1; i < argc; i++) { + + int parameterLength = (int)strlen(argv[i]); + + if(PARAMETER_CHECK("-i", 2, parameterLength)) { + if ((i+1) < argc) { + bedFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-g", 2, parameterLength)) { + if ((i+1) < argc) { + haveGenome = true; + genomeFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-excl", 5, parameterLength)) { + if ((i+1) < argc) { + haveExclude = true; + excludeFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-incl", 5, parameterLength)) { + if ((i+1) < argc) { + haveInclude = true; + includeFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-seed", 5, parameterLength)) { + if ((i+1) < argc) { + haveSeed = true; + seed = atoi(argv[i + 1]); + i++; + } + } + else if(PARAMETER_CHECK("-chrom", 6, parameterLength)) { + sameChrom = true; + } + else if(PARAMETER_CHECK("-f", 2, parameterLength)) { + if ((i+1) < argc) { + overlapFraction = atof(argv[i + 1]); + i++; + } + } + else { + cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; + showHelp = true; + } + } + + // make sure we have both input files + if (!haveBed || !haveGenome) { + cerr << endl << "*****" << endl << "*****ERROR: Need both a BED (-i) and a genome (-g) file. " << endl << "*****" << endl; + showHelp = true; + } + + if (haveInclude && haveExclude) { + cerr << endl << "*****" << endl << "*****ERROR: Cannot use -incl and -excl together." << endl << "*****" << endl; + showHelp = true; + } + + if (!showHelp) { + BedShuffle *bc = new BedShuffle(bedFile, genomeFile, excludeFile, includeFile, + haveSeed, haveExclude, haveInclude, sameChrom, + overlapFraction, seed); + delete bc; + return 0; + } + else { + ShowHelp(); + } +} + +void ShowHelp(void) { + + cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; + + cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; + + cerr << "Summary: Randomly permute the locations of a feature file among a genome." << endl << endl; + + cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -i <bed/gff/vcf> -g <genome>" << endl << endl; + + cerr << "Options: " << endl; + cerr << "\t-excl\t" << "A BED/GFF/VCF file of coordinates in which features in -i" << endl; + cerr << "\t\tshould not be placed (e.g. gaps.bed)." << endl << endl; + + cerr << "\t-incl\t" << "Instead of randomly placing features in a genome, the -incl" << endl; + cerr << "\t\toptions defines a BED/GFF/VCF file of coordinates in which " << endl; + cerr << "\t\tfeatures in -i should be randomly placed (e.g. genes.bed). " << endl << endl; + + cerr << "\t-chrom\t" << "Keep features in -i on the same chromosome."<< endl; + cerr << "\t\t- By default, the chrom and position are randomly chosen." << endl << endl; + + cerr << "\t-seed\t" << "Supply an integer seed for the shuffling." << endl; + cerr << "\t\t- By default, the seed is chosen automatically." << endl; + cerr << "\t\t- (INTEGER)" << endl << endl; + + cerr << "\t-f\t" << "Maximum overlap (as a fraction of the -i feature) with an -excl" << endl; + cerr << "\t\tfeature that is tolerated before searching for a new, " << endl; + cerr << "\t\trandomized locus. For example, -f 0.10 allows up to 10%" << endl; + cerr << "\t\tof a randomized feature to overlap with a given feature" << endl; + cerr << "\t\tin the -excl file. **Cannot be used with -incl file.**" << endl; + cerr << "\t\t- Default is 1E-9 (i.e., 1bp)." << endl; + cerr << "\t\t- FLOAT (e.g. 0.50)" << endl << endl; + + cerr << "Notes: " << endl; + cerr << "\t(1) The genome file should tab delimited and structured as follows:" << endl; + cerr << "\t <chromName><TAB><chromSize>" << endl << endl; + cerr << "\tFor example, Human (hg19):" << endl; + cerr << "\tchr1\t249250621" << endl; + cerr << "\tchr2\t243199373" << endl; + cerr << "\t..." << endl; + cerr << "\tchr18_gl000207_random\t4262" << endl << endl; + + + cerr << "Tips: " << endl; + cerr << "\tOne can use the UCSC Genome Browser's MySQL database to extract" << endl; + cerr << "\tchromosome sizes. For example, H. sapiens:" << endl << endl; + cerr << "\tmysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -e \\" << endl; + cerr << "\t\"select chrom, size from hg19.chromInfo\" > hg19.genome" << endl << endl; + + + // end the program here + exit(1); +}