Mercurial > repos > aaronquinlan > multi_intersect
diff BEDTools-Version-2.14.3/src/multiBamCov/multiBamCovMain.cpp @ 0:dfcd8b6c1bda
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author | aaronquinlan |
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date | Thu, 03 Nov 2011 10:25:04 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/BEDTools-Version-2.14.3/src/multiBamCov/multiBamCovMain.cpp Thu Nov 03 10:25:04 2011 -0400 @@ -0,0 +1,145 @@ +/***************************************************************************** + multiBamCovMain.cpp + + (c) 2009 - Aaron Quinlan + Hall Laboratory + Department of Biochemistry and Molecular Genetics + University of Virginia + aaronquinlan@gmail.com + + Licenced under the GNU General Public License 2.0 license. +******************************************************************************/ +#include "multiBamCov.h" +#include "version.h" + +using namespace std; + +// define our program name +#define PROGRAM_NAME "multiBamCov" + + +// define our parameter checking macro +#define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) + +// function declarations +void ShowHelp(void); + +int main(int argc, char* argv[]) { + + // our configuration variables + bool showHelp = false; + + // input files + string bedFile; + vector<string> bamFiles; + int minQual = 0; + + // input arguments + bool haveBed = false; + bool haveBams = false; + bool properOnly = false; + bool keepDuplicates = false; + bool keepFailedQC = false; + + // check to see if we should print out some help + if(argc <= 1) showHelp = true; + + for(int i = 1; i < argc; i++) { + int parameterLength = (int)strlen(argv[i]); + + if((PARAMETER_CHECK("-h", 2, parameterLength)) || + (PARAMETER_CHECK("--help", 5, parameterLength))) { + showHelp = true; + } + } + + if(showHelp) ShowHelp(); + + // do some parsing (all of these parameters require 2 strings) + for(int i = 1; i < argc; i++) { + + int parameterLength = (int)strlen(argv[i]); + + if(PARAMETER_CHECK("-bed", 4, parameterLength)) { + if ((i+1) < argc) { + haveBed = true; + bedFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-bams", 5, parameterLength)) { + if ((i+1) < argc) { + haveBams = true; + i = i+1; + string file = argv[i]; + while (file[0] != '-' && i < argc) { + bamFiles.push_back(file); + i++; + if (i < argc) + file = argv[i]; + } + i--; + } + } + else if(PARAMETER_CHECK("-q", 2, parameterLength)) { + if ((i+1) < argc) { + minQual = atoi(argv[i + 1]); + i++; + } + } + else if(PARAMETER_CHECK("-p", 2, parameterLength)) { + properOnly = true; + } + else if(PARAMETER_CHECK("-D", 2, parameterLength)) { + keepDuplicates = true; + } + + else if(PARAMETER_CHECK("-F", 2, parameterLength)) { + keepFailedQC = true; + } + else { + cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; + showHelp = true; + } + } + + if (!showHelp) { + MultiCovBam *mc = new MultiCovBam(bamFiles, bedFile, minQual, properOnly, keepDuplicates, keepFailedQC); + mc->CollectCoverage(); + delete mc; + return 0; + } + else { + ShowHelp(); + } +} + +void ShowHelp(void) { + + cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; + + cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; + + cerr << "Summary: Counts sequence coverage for multiple bams at specific loci." << endl << endl; + + cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -bams aln.1.bam aln.2.bam ... aln.n.bam -bed <bed/gff/vcf>" << endl << endl; + + cerr << "Options: " << endl; + + cerr << "\t-bams\t" << "The bam files." << endl << endl; + + cerr << "\t-bed\t" << "The bed file." << endl << endl; + + cerr << "\t-q\t" << "Minimum mapping quality allowed. Default is 0." << endl << endl; + + cerr << "\t-D\t" << "Include duplicate-marked reads. Default is to count non-duplicates only" << endl << endl; + + cerr << "\t-F\t" << "Include failed-QC reads. Default is to count pass-QC reads only" << endl << endl; + + cerr << "\t-p\t" << "Only count proper pairs. Default is to count all alignments with MAPQ" << endl; + cerr << "\t\t" << "greater than the -q argument, regardless of the BAM FLAG field." << endl << endl; + + // end the program here + exit(1); + +}