Mercurial > repos > aaronquinlan > multi_intersect
diff BEDTools-Version-2.14.3/src/fjoin/fjoinMain.cpp @ 0:dfcd8b6c1bda
Uploaded
author | aaronquinlan |
---|---|
date | Thu, 03 Nov 2011 10:25:04 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/BEDTools-Version-2.14.3/src/fjoin/fjoinMain.cpp Thu Nov 03 10:25:04 2011 -0400 @@ -0,0 +1,271 @@ +/***************************************************************************** + intersectMain.cpp + + (c) 2009 - Aaron Quinlan + Hall Laboratory + Department of Biochemistry and Molecular Genetics + University of Virginia + aaronquinlan@gmail.com + + Licenced under the GNU General Public License 2.0 license. +******************************************************************************/ +#include "fjoin.h" +#include "version.h" + +using namespace std; + +// define our program name +#define PROGRAM_NAME "fjoin" + + +// define our parameter checking macro +#define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) + +// function declarations +void ShowHelp(void); + +int main(int argc, char* argv[]) { + + // our configuration variables + bool showHelp = false; + + // input files + string bedAFile; + string bedBFile; + + // input arguments + float overlapFraction = 1E-9; + + bool haveBedA = false; + bool haveBedB = false; + bool noHit = false; + bool anyHit = false; + bool writeA = false; + bool writeB = false; + bool writeCount = false; + bool writeOverlap = false; + bool writeAllOverlap = false; + bool haveFraction = false; + bool reciprocalFraction = false; + bool forceStrand = false; + bool obeySplits = false; + bool inputIsBam = false; + bool outputIsBam = true; + + // check to see if we should print out some help + if(argc <= 1) showHelp = true; + + for(int i = 1; i < argc; i++) { + int parameterLength = (int)strlen(argv[i]); + + if((PARAMETER_CHECK("-h", 2, parameterLength)) || + (PARAMETER_CHECK("--help", 5, parameterLength))) { + showHelp = true; + } + } + + if(showHelp) ShowHelp(); + + // do some parsing (all of these parameters require 2 strings) + for(int i = 1; i < argc; i++) { + + int parameterLength = (int)strlen(argv[i]); + + if(PARAMETER_CHECK("-a", 2, parameterLength)) { + if ((i+1) < argc) { + haveBedA = true; + outputIsBam = false; + bedAFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-abam", 5, parameterLength)) { + if ((i+1) < argc) { + haveBedA = true; + inputIsBam = true; + bedAFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-b", 2, parameterLength)) { + if ((i+1) < argc) { + haveBedB = true; + bedBFile = argv[i + 1]; + i++; + } + } + else if(PARAMETER_CHECK("-bed", 4, parameterLength)) { + outputIsBam = false; + } + else if(PARAMETER_CHECK("-u", 2, parameterLength)) { + anyHit = true; + } + else if(PARAMETER_CHECK("-f", 2, parameterLength)) { + if ((i+1) < argc) { + haveFraction = true; + overlapFraction = atof(argv[i + 1]); + i++; + } + } + else if(PARAMETER_CHECK("-wa", 3, parameterLength)) { + writeA = true; + } + else if(PARAMETER_CHECK("-wb", 3, parameterLength)) { + writeB = true; + } + else if(PARAMETER_CHECK("-wo", 3, parameterLength)) { + writeOverlap = true; + } + else if(PARAMETER_CHECK("-wao", 4, parameterLength)) { + writeAllOverlap = true; + writeOverlap = true; + } + else if(PARAMETER_CHECK("-c", 2, parameterLength)) { + writeCount = true; + } + else if(PARAMETER_CHECK("-r", 2, parameterLength)) { + reciprocalFraction = true; + } + else if (PARAMETER_CHECK("-v", 2, parameterLength)) { + noHit = true; + } + else if (PARAMETER_CHECK("-s", 2, parameterLength)) { + forceStrand = true; + } + else if (PARAMETER_CHECK("-split", 6, parameterLength)) { + obeySplits = true; + } + else { + cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; + showHelp = true; + } + } + + // make sure we have both input files + if (!haveBedA || !haveBedB) { + cerr << endl << "*****" << endl << "*****ERROR: Need -a and -b files. " << endl << "*****" << endl; + showHelp = true; + } + + if (anyHit && noHit) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -u OR -v, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (writeB && writeCount) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -wb OR -c, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (writeCount && writeOverlap) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -wb OR -wo, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (writeA && writeOverlap) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -wa OR -wo, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (writeB && writeOverlap) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -wb OR -wo, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (reciprocalFraction && !haveFraction) { + cerr << endl << "*****" << endl << "*****ERROR: If using -r, you need to define -f." << endl << "*****" << endl; + showHelp = true; + } + + if (anyHit && writeCount) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -u OR -c, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (anyHit && writeB) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -u OR -wb, not both." << endl << "*****" << endl; + showHelp = true; + } + + if (anyHit && writeOverlap) { + cerr << endl << "*****" << endl << "*****ERROR: Request either -u OR -wo, not both." << endl << "*****" << endl; + showHelp = true; + } + + + if (!showHelp) { + + BedIntersect *bi = new BedIntersect(bedAFile, bedBFile, anyHit, writeA, writeB, writeOverlap, + writeAllOverlap, overlapFraction, noHit, writeCount, forceStrand, + reciprocalFraction, obeySplits, inputIsBam, outputIsBam); + delete bi; + return 0; + } + else { + ShowHelp(); + } +} + +void ShowHelp(void) { + + cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl; + + cerr << "Author: Aaron Quinlan (aaronquinlan@gmail.com)" << endl; + + cerr << "Summary: Report overlaps between two feature files." << endl << endl; + + cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -a <bed/gff/vcf> -b <bed/gff/vcf>" << endl << endl; + + cerr << "Options: " << endl; + + cerr << "\t-abam\t" << "The A input file is in BAM format. Output will be BAM as well." << endl << endl; + + cerr << "\t-bed\t" << "When using BAM input (-abam), write output as BED. The default" << endl; + cerr << "\t\tis to write output in BAM when using -abam." << endl << endl; + + cerr << "\t-wa\t" << "Write the original entry in A for each overlap." << endl << endl; + + cerr << "\t-wb\t" << "Write the original entry in B for each overlap." << endl; + cerr << "\t\t- Useful for knowing _what_ A overlaps. Restricted by -f and -r." << endl << endl; + + cerr << "\t-wo\t" << "Write the original A and B entries plus the number of base" << endl; + cerr << "\t\tpairs of overlap between the two features." << endl; + cerr << "\t\t- Overlaps restricted by -f and -r." << endl; + cerr << "\t\t Only A features with overlap are reported." << endl << endl; + + cerr << "\t-wao\t" << "Write the original A and B entries plus the number of base" << endl; + cerr << "\t\tpairs of overlap between the two features." << endl; + cerr << "\t\t- Overlapping features restricted by -f and -r." << endl; + cerr << "\t\t However, A features w/o overlap are also reported" << endl; + cerr << "\t\t with a NULL B feature and overlap = 0." << endl << endl; + + cerr << "\t-u\t" << "Write the original A entry _once_ if _any_ overlaps found in B." << endl; + cerr << "\t\t- In other words, just report the fact >=1 hit was found." << endl; + cerr << "\t\t- Overlaps restricted by -f and -r." << endl << endl; + + cerr << "\t-c\t" << "For each entry in A, report the number of overlaps with B." << endl; + cerr << "\t\t- Reports 0 for A entries that have no overlap with B." << endl; + cerr << "\t\t- Overlaps restricted by -f and -r." << endl << endl; + + cerr << "\t-v\t" << "Only report those entries in A that have _no overlaps_ with B." << endl; + cerr << "\t\t- Similar to \"grep -v\" (an homage)." << endl << endl; + + cerr << "\t-f\t" << "Minimum overlap required as a fraction of A." << endl; + cerr << "\t\t- Default is 1E-9 (i.e., 1bp)." << endl; + cerr << "\t\t- FLOAT (e.g. 0.50)" << endl << endl; + + cerr << "\t-r\t" << "Require that the fraction overlap be reciprocal for A and B." << endl; + cerr << "\t\t- In other words, if -f is 0.90 and -r is used, this requires" << endl; + cerr << "\t\t that B overlap 90% of A and A _also_ overlaps 90% of B." << endl << endl; + + cerr << "\t-s\t" << "Force strandedness. That is, only report hits in B that" << endl; + cerr << "\t\toverlap A on the same strand." << endl; + cerr << "\t\t- By default, overlaps are reported without respect to strand." << endl << endl; + + cerr << "\t-split\t" << "Treat \"split\" BAM or BED12 entries as distinct BED intervals." << endl << endl; + + + // end the program here + exit(1); + +}