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comparison BEDTools-Version-2.14.3/src/unionBedGraphs/unionBedGraphsMain.cpp @ 0:dfcd8b6c1bda

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author aaronquinlan
date Thu, 03 Nov 2011 10:25:04 -0400
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1 /*****************************************************************************
2 unionBedGraphsMain.cpp
3
4 (c) 2010 - Assaf Gordon, CSHL
5 - Aaron Quinlan, UVA
6 Hall Laboratory
7 Department of Biochemistry and Molecular Genetics
8 University of Virginia
9 aaronquinlan@gmail.com
10
11 Licenced under the GNU General Public License 2.0 license.
12 ******************************************************************************/
13 #include <climits>
14 #include <cstring>
15 #include <cstdlib>
16 #include <vector>
17 #include <string>
18 #include <iostream>
19 #include <getopt.h>
20 #include <libgen.h> //for basename()
21 #include "version.h"
22
23 #include "genomeFile.h"
24 #include "unionBedGraphs.h"
25
26 using namespace std;
27
28 // define our program name
29 #define PROGRAM_NAME "unionBedGraphs"
30
31 // define our parameter checking macro
32 #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen)
33
34 //STLized version of basename()
35 // (because POSIX basename() modifies the input string pointer)
36 // Additionally: removes any extension the basename might have.
37 std::string stl_basename(const std::string& path);
38
39 // function declarations
40 void ShowHelp(void);
41 void ShowExamples(void);
42
43
44 int main(int argc, char* argv[])
45 {
46 bool haveFiles = false;
47 bool haveTitles = false;
48 bool haveGenome = false;
49 bool haveFiller = true;
50 bool printHeader = false;
51 bool printEmptyRegions = false;
52 bool showHelp = false;
53 string genomeFile;
54 string basePath;
55 string noCoverageValue = "0";
56 vector<string> inputFiles;
57 vector<string> inputTitles;
58
59 //Parse command line options
60 if(argc <= 1)
61 ShowHelp();
62
63 for(int i = 1; i < argc; i++) {
64 int parameterLength = (int)strlen(argv[i]);
65
66 if((PARAMETER_CHECK("-h", 2, parameterLength)) ||
67 (PARAMETER_CHECK("--help", 5, parameterLength))) {
68 showHelp = true;
69 }
70 }
71
72 if(showHelp == true) {
73 ShowHelp();
74 exit(1);
75 }
76
77 // do some parsing (all of these parameters require 2 strings)
78 for(int i = 1; i < argc; i++) {
79
80 int parameterLength = (int)strlen(argv[i]);
81
82 if(PARAMETER_CHECK("-i", 2, parameterLength)) {
83 if ((i+1) < argc) {
84 haveFiles = true;
85 i = i+1;
86 string file = argv[i];
87 while (file[0] != '-' && i < argc) {
88 inputFiles.push_back(file);
89 i++;
90 if (i < argc)
91 file = argv[i];
92 }
93 i--;
94 }
95 }
96 else if(PARAMETER_CHECK("-names", 6, parameterLength)) {
97 if ((i+1) < argc) {
98 haveTitles = true;
99 i = i+1;
100 string title = argv[i];
101 while (title[0] != '-' && i < argc) {
102 inputTitles.push_back(title);
103 i++;
104 if (i < argc)
105 title = argv[i];
106 }
107 i--;
108 }
109 }
110 else if(PARAMETER_CHECK("-g", 2, parameterLength)) {
111 if ((i+1) < argc) {
112 haveGenome = true;
113 genomeFile = argv[i + 1];
114 i++;
115 }
116 }
117 else if(PARAMETER_CHECK("-filler", 7, parameterLength)) {
118 if ((i+1) < argc) {
119 haveFiller = true;
120 noCoverageValue = argv[i + 1];
121 i++;
122 }
123 }
124 else if(PARAMETER_CHECK("-header", 7, parameterLength)) {
125 printHeader = true;
126 }
127 else if(PARAMETER_CHECK("-empty", 6, parameterLength)) {
128 printEmptyRegions = true;
129 }
130 else if(PARAMETER_CHECK("-examples", 9, parameterLength)) {
131 ShowHelp();
132 ShowExamples();
133 exit(1);
134 }
135 }
136
137 //Sanity checks
138 if (inputFiles.empty() == true) {
139 cerr << "Error: missing BedGraph file names (-i) to combine." << endl;
140 exit(1);
141 }
142 if (inputFiles.size() == 1) {
143 cerr << "Error: Only a single BedGraph file was specified. Nothing to combine, exiting." << endl;
144 exit(1);
145 }
146 if (printEmptyRegions && (genomeFile.empty() == true)) {
147 cerr << "Error: when using -empty, the genome sizes file (-g) must be specified using '-g FILE'." << endl;
148 exit(1);
149 }
150 if ((haveTitles == true) && (inputFiles.size() != inputTitles.size())) {
151 cerr << "Error: The number of file titles (-names) does not match the number of files (-i)." << endl;
152 exit(1);
153 }
154
155 UnionBedGraphs ubg(cout, inputFiles, inputTitles, printEmptyRegions, genomeFile, noCoverageValue);
156 if (printHeader)
157 ubg.PrintHeader();
158 ubg.Union();
159 }
160
161 void ShowHelp(void) {
162
163 cerr << endl << "Program: " << PROGRAM_NAME << " (v" << VERSION << ")" << endl;
164
165 cerr << "Authors: Assaf Gordon, CSHL" << endl;
166 cerr << " Aaron Quinlan (aaronquinlan@gmail.com)" << endl << endl;
167
168 cerr << "Summary: Combines multiple BedGraph files into a single file," << endl;
169 cerr << "\t allowing coverage comparisons between them." << endl << endl;
170
171 cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -i FILE1 FILE2 .. FILEn" << endl;
172 cerr << "\t Assumes that each BedGraph file is sorted by chrom/start " << endl;
173 cerr << "\t and that the intervals in each are non-overlapping." << endl << endl;
174
175 cerr << "Options: " << endl;
176
177 cerr << "\t-header\t\t" << "Print a header line." << endl;
178 cerr << "\t\t\t(chrom/start/end + names of each file)." << endl << endl;
179
180 cerr << "\t-names\t\t" << "A list of names (one / file) to describe each file in -i." << endl;
181 cerr << "\t\t\tThese names will be printed in the header line." << endl << endl;
182
183 cerr << "\t-g\t\t" << "Use genome file to calculate empty regions." << endl;
184 cerr << "\t\t\t- STRING." << endl << endl;
185
186 cerr << "\t-empty\t\t" << "Report empty regions (i.e., start/end intervals w/o" << endl;
187 cerr << "\t\t\tvalues in all files)." << endl;
188 cerr << "\t\t\t- Requires the '-g FILE' parameter.\n" << endl;
189
190 cerr << "\t-filler TEXT\t" << "Use TEXT when representing intervals having no value." << endl;
191 cerr << "\t\t\t- Default is '0', but you can use 'N/A' or any other text." << endl << endl;
192
193 cerr << "\t-examples\t" << "Show detailed usage examples." << endl << endl;
194 }
195
196
197
198 void ShowExamples()
199 {
200 cerr << "Example usage:\n\n" \
201 "== Input files: ==\n" \
202 "\n" \
203 " $ cat 1.bg\n" \
204 " chr1 1000 1500 10\n" \
205 " chr1 2000 2100 20\n" \
206 "\n" \
207 " $ cat 2.bg\n" \
208 " chr1 900 1600 60\n" \
209 " chr1 1700 2050 50\n" \
210 "\n" \
211 " $ cat 3.bg\n" \
212 " chr1 1980 2070 80\n" \
213 " chr1 2090 2100 20\n" \
214 "\n" \
215 " $ cat sizes.txt\n" \
216 " chr1 5000\n" \
217 "\n" \
218 "== Union/combine the files: ==\n" \
219 "\n" \
220 " $ unionBedGraphs -i 1.bg 2.bg 3.bg\n" \
221 " chr1 900 1000 0 60 0\n" \
222 " chr1 1000 1500 10 60 0\n" \
223 " chr1 1500 1600 0 60 0\n" \
224 " chr1 1700 1980 0 50 0\n" \
225 " chr1 1980 2000 0 50 80\n" \
226 " chr1 2000 2050 20 50 80\n" \
227 " chr1 2050 2070 20 0 80\n" \
228 " chr1 2070 2090 20 0 0\n" \
229 " chr1 2090 2100 20 0 20\n" \
230 "\n" \
231 "== Union/combine the files, with a header line (titles are the file names): ==\n" \
232 "\n" \
233 " $ unionBedGraphs -header -i 1.bg 2.bg 3.bg\n" \
234 " chrom start end 1 2 3\n" \
235 " chr1 900 1000 0 60 0\n" \
236 " chr1 1000 1500 10 60 0\n" \
237 " chr1 1500 1600 0 60 0\n" \
238 " chr1 1700 1980 0 50 0\n" \
239 " chr1 1980 2000 0 50 80\n" \
240 " chr1 2000 2050 20 50 80\n" \
241 " chr1 2050 2070 20 0 80\n" \
242 " chr1 2070 2090 20 0 0\n" \
243 " chr1 2090 2100 20 0 20\n" \
244 "\n" \
245 "== Union/combine the files, with a header line and custom names: ==\n" \
246 "\n" \
247 " $ unionBedGraphs -header -i 1.bg 2.bg 3.bg -names WT-1 WT-2 KO-1\n" \
248 " chrom start end WT-1 WT-2 KO-1\n" \
249 " chr1 900 1000 0 60 0\n" \
250 " chr1 1000 1500 10 60 0\n" \
251 " chr1 1500 1600 0 60 0\n" \
252 " chr1 1700 1980 0 50 0\n" \
253 " chr1 1980 2000 0 50 80\n" \
254 " chr1 2000 2050 20 50 80\n" \
255 " chr1 2050 2070 20 0 80\n" \
256 " chr1 2070 2090 20 0 0\n" \
257 " chr1 2090 2100 20 0 20\n" \
258 "\n" \
259 "== Union/combine, showing empty regions (note, requires -g): ==\n" \
260 "\n" \
261 " $ unionBedGraphs -header -empty -g sizes.TXT -i 1.bg 2.bg 3.bg\n" \
262 " chrom start end 1 2 3\n" \
263 " chr1 0 900 0 0 0\n" \
264 " chr1 900 1000 0 60 0\n" \
265 " chr1 1000 1500 10 60 0\n" \
266 " chr1 1500 1600 0 60 0\n" \
267 " chr1 1600 1700 0 0 0\n" \
268 " chr1 1700 1980 0 50 0\n" \
269 " chr1 1980 2000 0 50 80\n" \
270 " chr1 2000 2050 20 50 80\n" \
271 " chr1 2050 2070 20 0 80\n" \
272 " chr1 2070 2090 20 0 0\n" \
273 " chr1 2090 2100 20 0 20\n" \
274 " chr1 2100 5000 0 0 0\n" \
275 "\n" \
276 ;
277 }
278
279 std::string stl_basename(const std::string& path)
280 {
281 string result;
282
283 char* path_dup = strdup(path.c_str());
284 char* basename_part = basename(path_dup);
285 result = basename_part;
286 free(path_dup);
287
288 size_t pos = result.find_last_of('.');
289 if (pos != string::npos )
290 result = result.substr(0,pos);
291
292 return result;
293 }
294